ALLELIC STATUS OF THE Ppd-1 AND VRN-1 GENETIC SYSTEMS IN WINTER WHEAT VARIETIES OF THE INSTITUTE OF IRRIGATED AGRICULTURE OF NAAS UKRAINE

Бакума, А. О.; Чеботар, Г. О.; Лавриненко, Ю. О.; Chebotar, S. V.

doi:10.18524/2077-1746.2019.1(44).168799

Cited by 3 publications

(2 citation statements)

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“…Only the variety Lehenda bilotserkivska is a carrier of the recessive allele Ppd-D1b and has a later heading date (Filimonov et al, 2018). In the Ppd-1 gene system of the varieties bred at the Institute of Irrigated Agriculture of NAAS the dominant Ppd-D1a allele there were identified and recessive b alleles were identified in the Ppd-A1 and Ppd-B1 loci (Bakuma et al, 2019). According to the results of our research, we assume that the new varieties of winter bread wheat of breeding at the MIW do not have the dominant allele of the Ppd-D1 gene, because varieties insensitive to the photoperiod were not detected among them.…”

Section: Discussionmentioning

confidence: 99%

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Klymets

Iskra

Svarchevska

2021

Regul. Mech. Biosyst.

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Dose-dependent changes in protein metabolism in the blood and hematological parameters of pregnant rats under the effect of vanadium citrate are presented in the article. The animals were divided into five groups: group I – non-pregnant females, II – pregnant females consuming pure water without additives, III, IV, V – females which during the mating and pregnancy period received the solution of vanadium citrate at concentrations of 0.03, 0.125 and 0.50 μg V/mL water. The research findings show that in pregnant animals of group II, the level of urea and alkaline phosphatase activity increased, meanwhile aspartate aminotransferase activity decreased, as compared to the non-pregnant females of group І. The levels of total protein and albumin decreased; however, the content of β-globulins increased in the pregnant animals of group II, as compared with that in group I. Also, in the rats of group II, there was a decrease in hemolysis time, total content of erythrocytes and hemoglobin, the content of old and mature erythrocytes, while the content of young erythrocytes increased, as compared to group I. The platelet content and thrombocrit in rats of group II increased in comparison with group I. The content of leukocytes and lymphocytes in pregnant animals of group II decreased, while the content of granulocytes increased, in contrast to non-pregnant rats. Under the effect of vanadium citrate at concentrations of 0.03–0.50 μg V/mL, there was a significant increase in the maximum number of prohemolized erythrocytes, the time of maximum hemolysis was delayed by 0.4–0.6 min, as compared with the pregnant rats of group II. This did not affect the time of total hemolysis in rats of groups III and V, as compared with the pregnant animals in group II. Under the effect of vanadium citrate, an increase in the content of young erythrocytes was observed, as compared with group II. The hemoglobin content decreased at the concentration of 0.125 μg V/mL, while at the concentration of 0.50 μg V/mL it increased, as compared to the pregnant animals of group II. Also, under the effect of vanadium citrate there was a decrease in the mean hemoglobin concentration in the erythrocyte. In pregnant animals fed with vanadium citrate solutions, the platelet content and thrombocrit, the relative width of platelet distribution by volume decreased, as compared with the pregnant rats of group II. The content of leukocytes, lymphocytes and granulocytes under the effect of vanadium citrate increased, as compared with the pregnant animals in group II. Under the effect of vanadium citrate at the concentration of 0.03 μg V/mL, the level of albumin, creatinine and aspartate aminotransferase activity increased in blood plasma in comparison with group II. Meanwhile, at the concentration of 0.125 μg V/mL, the relative content of γ-globulins and aspartate aminotransferase activity increased, alkaline phosphatase activity and urea level decreased in comparison with group II. However at the concentration of 0.50 μg V/mL, the relative α- and γ-globulins content and aspartate aminotransferase activity increased, at the same time, the relative β-globulins content and urea level decreased in comparison with group II. Therefore, vanadium citrate normalizes the indicators of protein metabolism during pregnancy, thus it can be considered as a potential dietary drug for the pregnant.

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Section: Discussionmentioning

confidence: 99%

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Klymets

Iskra

Svarchevska

2021

Regul. Mech. Biosyst.

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Section: Discussionmentioning

confidence: 99%

Features of modern winter wheat varieties in terms of winter hardiness components under conditions of Ukrainian Forest-Steppe

Pirych

Yurchenko

Hudzenko

et al. 2021

Regul. Mech. Biosyst.

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In recent years, there has been a significant change in climatic conditions affecting the cultivation and yield of winter wheat. Therefore, the creation of wheat varieties with high adaptive potential is one of the main tasks of modern breeding. A significant component of the overall adaptive potential of winter wheat is winter hardiness, which is determined by a set of characters enabling plants to overwinter. To a large extent, winter hardiness is determined by gene systems that control vernalization requirement duration, photoperiod reaction, and frost resistance. The research is aimed at determining the features of modern winter wheat varieties developed at the V. M. Remeslo Myronivka Institute of Wheat of the National Academy of Agrarian Sciences of Ukraine in terms of winter hardiness components and adaptive potential in the environment of the Central part of the Ukrainian Forest-Steppe. Winter bread wheat varieties Estafeta myronivska, Hratsiia myronivska, MIP Assol, and Balada myronivska were studied. They also were crossed on incomplete diallele scheme with three near-isogenic lines derived from Erythrospermum 604 with different alleles of Vrd genes 1) Vrd1Vrd1vrd2vrd2, 2) vrd1vrd1Vrd2Vrd2, and 3) vrd1vrd1vrd2vrd2. It was established that vernalization requirement duration in the varieties Estafeta myronivska and Balada myronivska was short whereas in the varieties Hratsiia myronivska and MIP Assol it was medium. All the varieties studied have medium photoperiod sensitivity. The results of the hybridological analysis indicate the absence of the Vrd1 and Vrd2 genes in the varieties. Frost tolerance of these varieties is at the same level and higher than in the highly tolerant to the low temperatures variety Myronivska 808. Thus, the results indicate the possibility of recombining different levels of expression of these traits in genotypes by breeding efforts. This has great practical importance in farming, because in recent years the areas of crops harvested late (corn, sunflower, etc.) in the production conditions has significantly increased. It causes a shift in sowing dates of winter wheat to a later period. In this case, varieties Estafeta myronivska, Hratsiia myronivska, MIP Assol, and Balada myronivska are able to undergo sufficient hardening, to satisfy the vernalization requirement, and to form a high level of winter hardiness. Their relatively medium photoperiod sensitivity allows vegetation to be restored a little earlier in the spring and winter reserves of moisture to be used more effectively.

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Alleles of Ppd-1 genes that control sensitivity to photoperiod in a number of bread winter wheat genotypes

Бакума¹,

Чеботар²,

Tkachuk³

et al. 2020

Plant Varieties Studying and Protection

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Purpose. Analysis of the allelic state of Ppd-1 genes, which control sensitivity to photoperiod, in varieties and lines of bread winter wheat, and comparison of the results obtained with field observations on the duration of periods before heading and flowering, whose originators were the Nosivska Breeding and Research Station of the V. M. Remeslo Myronivka Institute of Wheat National Academy of Agrarian Sciences of Ukraine and Poltava State Agrarian Academy of the Ministry of Education and Science of Ukraine. Methods. The following methods were used in the work: DNA extraction, allele-specific PCR, agarose and polyacrylamide gel electrophoresis, analysis of variance. Results. It was revealed that 'Yuvivata 60' variety has a recessive Ppd-1 genotype and belongs to the III haplotype by a combination of mutations in the structure of Ppd-D1 gene. Line 'L41/95' was heterogeneous by alleles of Ppd-D1 gene, which corresponded to the presence of haplotypes III and VII. All other tested cultivars and lines were characterized by alleles Ppd-A1b, Ppd-B1b and Ppd-D1a, and assigned to haplotype VII. According to the results of statistical data processing, the duration of the period from May, 1 to heading was the smallest for the variety 'Donskaya polukarlikovaya' in the conditions of both the Forest-Steppe and Polissia-Forest-Steppe regions of Ukraine, the longest-in the varieties 'Yuvivata 60', 'Myronivska 61' and 'L41/95'. The differences between these groups were significant and amounted to 10 days. Conclusions. A breeding material with a high adaptive ability for growing conditions in Polissia-Forest-Steppe zone was studied by the allelic state of the Ppd-1 genes. A low level of polymorphism in the studied varieties and lines was revealed by the alleles of Ppd-1 genes [12.5%-Ppd-D1b (²²²), 12.5%-Ppd-D1à/b (²²²/VII), 75%-Ppd-D1a (VII)], that agrees with the hypothesis that breeders gave a greater preference for the photoperiod-insensitive wheat genotype under Ukrainian conditions. The genotypes with the dominant Ppd-D1a (VII) gene almost completely dominate in the south of Ukraine. At the same time, in northern latitudes, weather conditions negate the advantages of the genotypes with Ppd-D1a gene.

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ALLELIC STATUS OF THE Ppd-1 AND VRN-1 GENETIC SYSTEMS IN WINTER WHEAT VARIETIES OF THE INSTITUTE OF IRRIGATED AGRICULTURE OF NAAS UKRAINE

Cited by 3 publications

References 25 publications

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Features of modern winter wheat varieties in terms of winter hardiness components under conditions of Ukrainian Forest-Steppe

Alleles of Ppd-1 genes that control sensitivity to photoperiod in a number of bread winter wheat genotypes

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