Allelic variation at Fr-H1/Vrn-H1 and Fr-H2 loci is the main determinant of frost tolerance in spring barley

Tondelli, Alessandro; Pagani, D.; Ghafoori, Iman Naseh; Rahimi, Mehdi; Ataei, Reza; Rizza, Fulvia; Flavell, Andrew J.; Cattivelli, Luigi

doi:10.1016/j.envexpbot.2014.02.014

Cited by 23 publications

(17 citation statements)

References 36 publications

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“…Sequence variation in VRN genes underlies the differences in vernalization requirement between spring-and autumn-sown cereals 40,41 . VRN-H1 has been implicated in frost tolerance in spring barley 42 . Our results suggest that allelic diversity at VRN-H1 and VRN-H3 correlates with flowering-time variation in spring-sown barleys.…”

Section: Nature Geneticsmentioning

confidence: 99%

Genebank genomics highlights the diversity of a global barley collection

et al. 2018

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Genebanks hold comprehensive collections of cultivars, landraces and crop wild relatives of all major food crops, but their detailed characterization has so far been limited to sparse core sets. The analysis of genome-wide genotyping-by-sequencing data for almost all barley accessions of the German ex situ genebank provides insights into the global population structure of domesticated barley and points out redundancies and coverage gaps in one of the world's major genebanks. Our large sample size and dense marker data afford great power for genome-wide association scans. We detect known and novel loci underlying morphological traits differentiating barley genepools, find evidence for convergent selection for barbless awns in barley and rice and show that a major-effect resistance locus conferring resistance to bymovirus infection has been favored by traditional farmers. This study outlines future directions for genomics-assisted genebank management and the utilization of germplasm collections for linking natural variation to human selection during crop evolution.

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Section: Nature Geneticsmentioning

confidence: 99%

Genebank genomics highlights the diversity of a global barley collection

et al. 2018

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“…Under cold stress, plants have a short-term response including cold acclimation, and a long-term response, which involves developmental responses such as vernalization (Kosova et al 2011;Pecchioni et al 2014). Barley can be classified into spring, facultative, and winter types depending on its growth habitat, tolerance to low temperature, requirements for vernalization, and sensitivity to photoperiod (Tondelli et al 2014). Photoperiod sensitivity and vernalization play roles in low-temperature tolerance by delaying the transition from the vegetative to the reproductive stage, thus delaying flowering, which allows plants to survive low-temperature stress (Fowler et al 2001;Fowler et al 1996a;Fowler et al 1996b;Mahfoozi et al 2001a;Mahfoozi et al 2001b).…”

Section: Low-temperature Stressmentioning

confidence: 99%

“…Interestingly, FR-H1 was found to overlap with the VRN-H1 vernalization locus. Whether this association of both vernalization requirement and freezing tolerance is a result of having linked genes (Francia et al 2004;Hayes et al 1993) or having one gene with pleiotropic effects (Tondelli et al 2014;von Zitzewitz et al 2011) is still a debatable question (Fisk et al 2013). If one considers the pleiotropy effect, then HvBM5A would be the candidate gene underlying VRN-H1 and FR-H1 (von Zitzewitz et al 2005).…”

Section: Low-temperature Stressmentioning

confidence: 99%

“…The involvement of FR-H1 and FR-H2 in cold acclimation was detected using a genome-wide association mapping approach, where the presence of specific alleles (winter alleles) at these loci conferred maximum low-temperature tolerance (von Zitzewitz et al 2011). The allelic variation at FR-H1 and FR-H2 was also the main determinant of frost tolerance in a panel of a European two-row spring barley population (Tondelli et al 2014). Besides FR-H1 and FR-H2, (Fisk et al 2013) mapped another QTL, FR-H3, which is associated with freezing tolerance on the short arm of chromosome 1H.…”

Section: Low-temperature Stressmentioning

confidence: 99%

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Genomic and Genetic Studies of Abiotic Stress Tolerance in Barley

Saadé

Negrão

Plett

et al. 2018

Compendium of Plant Genomes

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Barley is a resilient crop plant with higher tolerance than other cereal plants for several types of abiotic stress. In this chapter, we describe the genetic components underlying barley's response to abiotic stresses, including soil acidity, boron toxicity, soil salinity, drought, temperature, and nutrient deficiency. We describe typical symptoms observed in barley in response to these stresses. We enumerate the major qualitative trait loci (QTLs) identified so far, such as FR-H1 and FR-H2 for low-temperature tolerance. We also discuss candidate genes that are the basis for stress tolerance, such as HVP10, which underlies the HvNax3 locus for salinity tolerance. Although knowledge about barley's responses to abiotic stresses is far from complete, the genetic diversity in cultivated barley and its close wild relatives could be further exploited to improve stress tolerance. To this end, the release of the barley high-quality reference genome provides a powerful tool to facilitate identification of new genes underlying barley's relatively high tolerance to several abiotic stresses. Early research on the genetic control of Al tolerance in barley using the Dayton (Al-tolerant) and Smooth Awn 86 (Al-sensitive) genotypes detected a major locus, Alp, on the long arm of chromosome 4H (4HL) (Minella and Sorrells 1992; Reid 1971). Another early study found that a single gene, named Pht, on

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“…Another common characteristics of the genome of grasses is the organization of CBFs in syntenic clusters of tandemly duplicated paralogs that belong to the phylogenetic subgroups CBF3/CBFIII and CBF4/CBFIV (Badawi et al 2007;Tondelli et al 2011). In barley, quantitative genetic studies have demonstrated that a large part of the observed phenotypic variation in frost resistance is explained by two major loci (QTL), FR-H1 and FR-H2, located approximately 25 cM apart on chromosome 5 H (Francia et al 2004;von Zitzewitz et al 2011;Tondelli et al 2014). FR-H1 overlaps the VRN-H1 vernalization response locus; FR-H2 segregates with a cluster of at least 13 CBFs, co-localizes with QTL influencing COR protein accumulation, and correlates with mRNA levels of the CBF genes within the locus (Francia et al 2004Stockinger et al …”

mentioning

confidence: 99%

Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

Francia

Morcia²,

Pasquariello

et al. 2016

Plant Mol Biol

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A family of CBF transcription factors plays a major role in reconfiguring the plant transcriptome in response to low-freezing temperature in temperate cereals. In barley, more than 13 HvCBF genes map coincident with the major QTL FR-H2 suggesting them as candidates to explain the function of the locus. Variation in copy number (CNV) of specific HvCBFs was assayed in a panel of 41 barley genotypes using RT-qPCR. Taking advantage of an accurate phenotyping that combined Fv/Fm and field survival, resistance-associated variants within FR-H2 were identified. Genotypes with an increased copy number of HvCBF4 and HvCBF2 (at least ten and eight copies, respectively) showed greater frost resistance. A CAPS marker able to distinguish the CBF2A, CBF2B and CBF2A/B forms was developed and showed that all the higher-ranking genotypes in term of resistance harbour only CBF2A, while other resistant winter genotypes harbour also CBF2B, although at a lower CNV. In addition to the major involvement of the HvCBF4-HvCBF2 genomic segment in the proximal cluster of CBF elements, a negative role of HvCBF3 in the distal cluster was identified. Multiple linear regression models taking into account allelic variation at FR-H1/VRN-H1 explained 0.434 and 0.550 (both at p < 0.001) of the phenotypic variation for Fv/Fm and field survival respectively, while no interaction effect between CNV at the HvCBFs and FR-H1/VRN-H1 was found. Altogether our data suggest a major involvement of the CBF genes located in the proximal cluster, with no apparent involvement of the central cluster contrary to what was reported for wheat

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Allelic variation at Fr-H1/Vrn-H1 and Fr-H2 loci is the main determinant of frost tolerance in spring barley

Cited by 23 publications

References 36 publications

Genebank genomics highlights the diversity of a global barley collection

Genebank genomics highlights the diversity of a global barley collection

Genomic and Genetic Studies of Abiotic Stress Tolerance in Barley

Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

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