Allelic variation in the DQ subregion of the canine major histocompatibility complex: II. DQB

Sarmiento, UllaM.; DeRose, Susan; Sarmiento, JuanI.; Storb, Rainer

doi:10.1007/bf00216840

Cited by 21 publications

(10 citation statements)

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“…Exon 2 contained 267 nucleotides and encodes most of the P l domain. Some of the alleles may be the same as those published previously (8,27). In these publications, different primers were used and sequence was amplified from mRNA.…”

Section: Discussionmentioning

confidence: 98%

“…The same phylogenetic tree as in Fig. 8 showing the bootstrap confidence level (%) calculated using the MEGA program (13) isms in dogs and humans are also present in exon 3, but in humans the DQB alleles can be distinguished by the exon 2 sequence (8,28). In this analysis, the polymorphism was localized to five hypervariable areas at amino acid positions 9-14, 25-35, 57-62, 66-77, and 54-58, which are similar to the areas of polymorphism in DLA-DRBl and HLA-DQB1 (19,29).…”

Section: Discussionmentioning

confidence: 99%

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Molecular analysis and polymorphism of the DLA‐DQB genes

et al. 1998

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Partial-length cDNA clones and full-length genomic clones corresponding to a complete canine DQB class II gene were isolated. Southern analyses suggested the presence of two DQB genes--one of which appeared to be a pseudogene lacking exon 2 called DQB2. The other DQB gene, called DQB1, was isolated from a genomic phage clone and contained six exons. The DQB1 clone was restriction mapped, and exon 2 was sequenced from 70 dogs. Twenty alleles were found. Most of the amino acid substitutions occurred at putative positions in the peptide binding site. Inheritance of these sequences showed Mendelian segregation with one or two alleles per dog. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the canine DQB1 alleles into four major allelic groups. The number of nonsynonymous changes was higher than the number of synonymous changes in the putative antigen recognition sites suggestive of positive selection.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Molecular analysis and polymorphism of the DLA‐DQB genes

et al. 1998

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“…MHC haplotypes in dogs have been defined by the combination of alleles at the variable second exon of three functional class II genes: DLA‐DRB1 , DLA‐DQA1 and DLA‐DQB1 . This is largely because screening efforts have focused on this exon, which encodes much of the functional peptide binding region (PBR) domain, and in the class II region (7–9). Particular class II haplotypes have been isolated in otherwise divergent dog breeds from widespread geographical locations, suggesting that these combinations are ancestral and/or are selectively favourable combinations of alleles (5).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary history of DLA class II haplotypes in canine diabetes mellitus through single nucleotide polymorphism genotyping

2010

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Strong linkage disequilibrium (LD) is a characteristic of the major histocompatibility complex (MHC) region, as well as the genome in general in dogs as a consequence of demographic changes with domestication. Disease association studies of MHC haplotypes may be affected by high LD and the resultant shared genetic backgrounds of haplotypes giving associations with linked but non-causative mutations, and also by convergent haplotypes, in which combinations of alleles have arisen independently. This study provides preliminary tools for dog leukocyte antigen (DLA) class II haplotype analysis with 102 single nucleotide polymorphisms (SNPs) identified in 14.6 kb and genotyping of 20 of these SNPs to tag haplotypes in 60 dogs with diabetes mellitus and in 49 non-diabetic dogs. The pattern of LD and analysis of SNP patterns indicated combinations of exon 2 alleles have arisen through both recombination and convergence. For exon 2 haplotypes associated with susceptibility or protection from diabetes mellitus, a region of fixed differences in SNPs across the DQ region was observed, suggesting a region outside exon 2 may be implicated in disease association. Four new DQB1 promoter alleles restricted to diabetic dogs were identified, as well as a substitution difference in the X1 box of the DQB1 promoter that will potentially modify the effect of the protective haplotypes within diabetic dogs.

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“…These genes were selected because trans-species conservation of class II MHC sequences has been shown in other mammals Erlich and Gyllensten 1991;Fan 1989;Gustafsson et al 1990; Kupfermann et al 1992;Slierendregt et al 1992;Yaeger and Hughes 1999). While there is limited information regarding the MHC of species closely related to the California sea lion (order/suborder Camivora/Caniformia/ Pinnipedia) (McKenna and Bell 1997), the class II MHC genes of a related terrestrial carnivore, the domestic dog (order/suborder Camivora/Caniformia/Canidae); Schreiber et al 1998), have been extensively studied (Polvi et al 1997;Sarmiento et al 1992Sarmiento et al , 1993Wagner et al 1996Wagner et al , 1998Wagner et al , 1999. These studies show that the canine DQA and DQB gene products are polymorphic, and may be important in generating peptide-binding diversity.…”

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confidence: 99%

Molecular characterization of expressed DQA and DQB genes in the California sea lion ( Zalophus californianus )

et al. 2002

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To date, there are no published MHC sequences from the California sea lion (Zalophus califomianus), a thriving species that, by feeding high on the marine food web, could be a sentinel for disturbances in marine and coastal ecosystems. In this study, degenerate primers and RACE technology were used to amplify near-fiiUThis study was supported by grants from the Office of Naval Resources (ONR), the Marine Community Foundation, the Fogarty International Center and the US National Science Foundation (#DEB-0094919) Studies were performed jointly in the laboratories of Stott and JohnsonThe nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the following accession numbers: An additional three Zaca-DQB sequences containing features compatible with pseudogenes or null alleles were also identified. Despite the identification of multiple DQA and DQB sequences, the degree of heterogeneity between them was extremely low. To confirm the limited degree of Zaca-DQ nucleotide variation between individuals, we used denaturing gradient gel electrophoresis to examine putative peptide binding region sequences from the peripheral blood leukocyte-derived RNAs of 19 wild-caught California sea lions from physically distinct populations. The pattern of Zaca-DQ sequence migration was identical between individuals and independent of geographical region. This apparent Zaca-DQ sequence identity between sea lions was confirmed by direct sequencing of individual bands. In combination, these fmdings raise important questions regarding immimogenetic diversity within this thriving species, and should prompt further research into the existence of a highly polymorphic sea lion class II MHC molecule with sequence features that support traditional peptide binding fiuictions.

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Allelic variation in the DQ subregion of the canine major histocompatibility complex: II. DQB

Cited by 21 publications

References 0 publications

Molecular analysis and polymorphism of the DLA‐DQB genes

Molecular analysis and polymorphism of the DLA‐DQB genes

Evolutionary history of DLA class II haplotypes in canine diabetes mellitus through single nucleotide polymorphism genotyping

Molecular characterization of expressed DQA and DQB genes in the California sea lion ( Zalophus californianus )

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