Allelopathic effect of medicinal plant Cannabis sativa L. on Lactuca sativa L. seed germination

Abstract

In order to examine allelopathic effect of Cannabis sativa L. on germination capability and seedling growth of Lactuca sativa L., a study was performed in laboratory conditions. Treatments were set up in randomised block design in four replications for each of four concentration ranges of 25, 50, 75 and 100 % of aqueous extract made of shoot parts and 4 identical extract concentrations made of root of cannabis. Control variant was lettuce seed treated by distilled water. During the studies shoot and seminal root length of lettuce seedlings were measured after treatments with different concentrations of extracts made of root and shoot parts of cannabis, and the obtained values were compared with the control. The obtained results suggest that the extract from the shoot parts of cannabis in high concentrations of 75 and 100% had inhibiting effect to the germination indices while the extract from the root had no statistically significant effect on germination of lettuce seeds. Extract made of root part of cannabis showed also stimulatory effect to shoot and seminal root length of lettuce seedlings in extract concentrations of 50, 75 and 100 %. V laboratorijskih razmerah je bil preučevan alelopatičen učinek navadne konoplje (Cannabis sativa L.) na kalitev semen in rast kalic vrtne solate (Lactuca sativa L.). Obravnavanja so bila izvedena kot naključni bločni poskus v štirih ponovitvah z vsako od štirih koncentracij, 25, 50, 75 in100 %, vodnega izvlečka poganjkov in korenin konoplje. V kontrolnem poskusu so bila semena vrtne solate tretirana na enak način z distilirano vodo. Poleg indeksa kalitve sta bili v poskusi merjeni še dolžina poganjkov in semenskih korenin kaleče solate. Rezultati so pokazali, da je imel izvleček poganjkov konoplje v večjih koncentracijah, 75 in 100 %, inhibitorni učinek na kalitveni indeks, medtemko izvlečki iz korenin niso imeli statistično značilnega vpliva na iste merjene parametre. Izvleček iz korenin konoplje je imel stimulativni učinek na dolžino poganjkov in semenskih korenin vrtne solate pri koncentracijah 50, 75 in 100 %.

Allelopathic effects of dominant ground vegetation species on initial growth of Pinus sylvestris L. seedlings in response to different temperature scenarios

“…These results match with those observed in previous studies showing that increased temperature stimulates the production of higher allelochemical concentrations, which could have stronger effects on acceptor species (Wang et al 2011, Zhang et al 2014. However, our results indicated a different response of the root and shoot extracts of dominant species to seed germination at all temperatures compared with the data obtained in previous studies (Mahmoodzadeh et al 2015, Gulzar & Siddiqui 2014. A weaker inhibition of root extracts has been specified for other plant species, for example Heracleum mantegazzianum (Apiaceae -Balezentiene & Renćo 2014).…”

“…The different allelopathic effects of the shoots and roots of dominant species has also been documented by other authors (Gulzar & Siddiqui 2014). Mahmoodzadeh et al (2015) noted that the different qualitative composition and quantitative content of biologically active compounds might explain the different effects of shoots and roots of dominant species. Moreover, the inhibitory effect of extracts from different parts of plant dominants on seed germination at all temperatures might be due to imbalance in metabolism regulated by various enzyme activities (Oyun 2006).…”

Antimicrobial, Antioxidant, and Antiproliferative Effects of Coronilla minima: An Unexplored Botanical Species

“…The second assay measures the survival of brine shrimp (Artemia salina) cysts after challenging with extracts. The complete description of each test is reported in previous papers [25,26].…”

Phytotoxic potential of selected medicinal plants on germination of Lactuca sativa seeds

“…It is also reported that the chemicals of different part of the same plant can showed phytotoxic, inhibitory or stimulatory effects on other recipient plants. Mehmoodzadeh et al [25] reported the allelopathic effect of Cannabis sativa shoot and root extract where shoot showed inhibitory effects while root gave stimulatory effects on seeds of Lactuca sativa. Present study showed that at 20mg radicle length of Lactuca sativa was least with Monotheca buxifolia (0.12), followed by Cucumis sativus (0.14) and Malus baccata (0.63).…”