Allergen action levels for food industries as risk management tools for a better use of precautionary Allergen labelling

Manny, Emilie; Dominguez, Silvia A.; Barrère, Virginie; Théolier, Jérémie; Godefroy, Samuel Benrejeb

doi:10.1016/j.foodcont.2021.108773

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…The LODs of the four allergens vary from 0.3 to 7.8 ng/mL, significantly lower than the reference doses for precautionary labeling and action level II thresholds that may trigger an acute food allergy (Table ). Distinctions in their LODs are probably due to the magnitude of titer values, which reflect the binding affinity between the antibody and the corresponding antigen. It was reported that the titer value of anti-TM monoclonal antibody is ∼1 × 10 6 , while that of anti-OVA monoclonal antibody is only ∼2.4 × 10 4 , resulting in the lower dissociation constant of TM-based ELISA product and more sensitive detection of TM (0.31 ng/mL) than OVA (7.8 ng/mL).…”

Section: Resultsmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens

et al. 2023

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Food allergy has become a growing health concern that may impair life quality and even cause life-threatening outcomes. Accidental and continuous exposure to allergenic bioaerosols has a substantially negative impact on the respiratory health of patients. Traditional analytical methodologies for food allergens are restricted by strong reliance on bulk instrumentation and skilled personnel, particularly in low-resource settings. In this study, a fluorescent sensor array based on the enzyme-linked immunosorbent assay performed on a herringbone-shaped microfluidic chip (ELISA-HB-chip) was designed for dynamically sensitive and multiplexed quantification of foodborne allergens in aerosols that originated from liquid food extracts. Due to the high surface area of aerosol particles and sufficient mixing of immunological reagents using a herringbone micromixer, the detection sensitivity was improved by over an order of magnitude compared to traditional allergen detection in the aqueous phase. Through fluorescence imaging of multiple regions on the ELISA-HB-chip, four important foodborne allergens, namely, ovalbumin, ovomucoid, lysozyme, and tropomyosin, could be simultaneously monitored without any cross-reactivity, and the limits of detection for these allergenic species were determined to be 7.8, 1.2, 4.2, and 0.31 ng/mL, respectively. Combining with a 3D printed and portable fluorescence microscope, this platform exhibited an excellent field-deployable capacity for quick and accurate determination of allergens in the aerosol state from spiked buffer solutions, thus displaying the practicality for food safety screening at cooking or food processing sites where patients are potentially under exposure to allergenic bioaerosols that escaped from food matrices or extracts.

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Section: Resultsmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens

et al. 2023

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“…More and more researchers are paying attention to food allergies for their lethal effects on allergic consumers (2,3). It is reported that 5% of adults and 8% of young children suffer from food allergies, while more than 90% of food allergies are caused by milk, peanuts, eggs, soybeans, wheat, nuts, fish, and crustaceans (4)(5)(6)(7)(8)(9). At the very top of the list of food allergens, shellfish is commonly identified as a cause of food hypersensitivity toward sensitized individuals (10).…”

Section: Introductionmentioning

confidence: 99%

Determination of Tropomyosin in Shrimp and Crab by Liquid Chromatography–Tandem Mass Spectrometry Based on Immunoaffinity Purification

Fan

et al. 2022

Front. Nutr.

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A UPLC-MS/MS method was developed for the detection of tropomyosin (TM) in shrimp and crab. After simple extraction, the samples were purified by immunoaffinity column and then digested by trypsin. The obtained sample was separated by Easy-nLC 1000-Q Exactive. The obtained spectrums were analyzed by Thermo Proteome Discoverer 1.4 software and then ANIQLVEK with high sensitivity was selected as the quantitative signature peptide. Isotope-labeled internal standard was used in the quantitative analysis. The method showed good linearity in the range of 5–5,000 μg/L with a limit of quantification (LOQ) of 0.1 mg/kg. The average recoveries were 77.22–95.66% with RSDs ≤ 9.97%, and the matrix effects were between 88.53 and 112.60%. This method could be used for rapid screening and quantitative analysis of TM in shrimp and crab. Thus, it could provide technical support for self-testing of TM by food manufacturers and promote further improvement of allergen labeling in China.

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Specific detection of crustacean allergens in food: Development of indirect competitive and sandwich ELISA targeting sarcoplasmic calcium binding protein

Zhu,

Qin,

Huang

et al. 2024

Food Bioscience

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Allergen action levels for food industries as risk management tools for a better use of precautionary Allergen labelling

Cited by 4 publications

References 24 publications

Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens

Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens

Determination of Tropomyosin in Shrimp and Crab by Liquid Chromatography–Tandem Mass Spectrometry Based on Immunoaffinity Purification

Specific detection of crustacean allergens in food: Development of indirect competitive and sandwich ELISA targeting sarcoplasmic calcium binding protein

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