Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches

Salazar, Alberto; Velázquez-Soto, Henry; Ayala-Balboa, Julio; Jiménez-Martínez, María del Carmen

doi:10.5772/intechopen.69276

Cited by 6 publications

(15 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Any atypical reactions after the consumption of food, such as food hypersensitivity, including food intolerances and food allergies or food aversions, are defined as adverse food reactions [1]. Food intolerance (FI) affects up to 20% of the world's population, but the true prevalence remains unknown due to a data deficiency [2] and the lack of a standardized assessment for FI [3]. Routine clinical tests for FI can be conditionally divided Data 2021, 6, 122 2 of 9 into two types: cellular (the study of changes in blood cells) and humoral (the study of the level of antibodies specific to food antigens (AGs)) [4].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Specific IgG to Identify Possible Food Intolerance in Athletes Using ELISA

Malsagova¹,

Stepanov²,

Sinitsyna³

et al. 2021

Data

View full text Add to dashboard Cite

Nutrition is considered one of the foundations of athletic performance, and post-workout nutritional recommendations are fundamental to the effectiveness of the recovery and adaptive processes. Therefore, at present, new directions in dietetics are being formed, focused on the creation of personalized diets. To identify the probable risk of somatic and allergic reactions upon contact with food antigens, we used the method of enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of IgG antibodies in the blood plasma of athletes against protein–peptide antigens accommodated in food. The study enrolled 40 athletes of boating and fighting sport disciplines. We found that the majority of the studied participants were characterized by an elevated IgG level against one or two food allergens (barley, almond, strawberry, etc.). Comparative analysis of the semiquantitative levels of IgG antibodies in athletes engaged in boating and fighting did not reveal significant differences between these groups. As a result, foods that are likely to cause the most pronounced immune response amongst the studied participants can be identified, which may indicate the presence of food intolerances. An athlete’s diet is influenced by both external and internal factors that can reduce or worsen the symptoms of a food intolerance/allergy associated with exercise. The range of foods is wide, and the effectiveness of a diet depends on the time, the place, and environmental factors. Therefore, during the recovery period (the post-competition period), athletes are advised to follow the instructions of doctors and nutritionists. An effective, comprehensive recovery strategy during the recovery period may enhance the adaptive response to fatigue, improving muscle function and increasing exercise tolerance. The data obtained may be useful for guiding the development of a new personalized approach and dietary recommendations covering the composition of athletes’ diet and the prevalence of food intolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In ELISA, the diagnostic marker is a measure of antigen-specific immunoglobulins of class G (IgG) or subclass G4 (IgG4) [12]. In addition, approaches such as the immunological solid-phase allergen chip (ImmunoCAP-ISAC) and Western blot are based on the ELISA approach [3].…”

Section: Discussionmentioning

confidence: 99%

Determination of Specific IgG to Identify Possible Food Intolerance in Athletes Using ELISA

Malsagova¹,

Stepanov²,

Sinitsyna³

et al. 2021

Data

View full text Add to dashboard Cite

show abstract

“…The evolution of methods for IgE diagnostic comprises methods like Radio-Immuno-Sorbent-Test (RAST), Paper-Radio-Immuno-Sorbent-Test (PRIST), and Enzyme-Linked Immuno-Sorbent-Assay (ELISA), gave rise to more reliable, safe, and automatized methods. A deeper revision of these methodologies could be found in [6].…”

Section: Methodologies For Tige and Sige Determinationmentioning

confidence: 99%

“…For tIgE detection, an anti-IgE antibody (detection antibody) will bind to the fragment crystallizable region in the immunoglobulin E. For sIgE, the serum sample is incubated with the allergen-coated surface before incubation with the detection antibody, thus allowing allergen-specific IgE to be detected. Finally, the reaction is detected according to the platform methodology: radiation, colorimetry, fluorometry, or chemiluminescence [6][7][8].…”

Section: Principles Of Laboratory Testing For Molecular Allergymentioning

confidence: 99%

Fundamentals of Molecular Allergy: From Bench to Bedside

Velázquez-Soto¹,

Martinez²

2023

Allergic Disease - New Developments in Diagnosis and Therapy

Self Cite

View full text Add to dashboard Cite

This chapter describes the fundamentals of molecular allergy diagnosis and raises the concept of allergens, allergenic components, and recombinant allergens. In addition, the authors review quality aspects related to the laboratory methodology. In the last part of the chapter, the different singleplex and multiplex platforms currently used for molecular diagnosis are compared. Finally, the diagnostic systems’ challenges, strengths, and pitfalls are discussed to understand their clinical impact. Undoubtedly, this chapter will be handy for the background knowledge for health personnel, allergists/immunologists, and clinical laboratory personnel to guide the selection of diagnostic tests for allergy as well as their interpretation and therapeutic approach.

show abstract

“…Bajo esta premisa, es posible identificar y cuantificar la concentración de IgE total ( T IgE) e IgE específica ( S IgE) a ciertos alergenos a través de la reacción antígeno-anticuerpo. 5 En todas las metodologías de detección de anticuerpos, la IgE en el suero del paciente será el antígeno para cuantificar y éste será reconocido a través de un segundo anticuerpo formando un complejo Ag-Ab. Para llevar a cabo estos ensayos, un anticuerpo específico para la fracción Fc de la IgE deberá ser adsorbido a una fase sólida, generalmente en superficies de poliestireno o celulosa.…”

Section: Introductionunclassified

Capítulo 2. Diagnóstico molecular

Jiménez-Martínez¹,

Velázquez-Soto²

2022

Alergia, Asma E Inmunología Pediátricas

Self Cite

View full text Add to dashboard Cite

INTRODUCCIÓNLas pruebas diagnósticas de alergia han evolucionado a la par de la tecnología biomédica y del conocimiento de los componentes alergénicos, de tal forma que han revolucionado el diagnóstico médico. En este sentido podemos definir el diagnóstico molecular de alergia como el uso de tecnologías basadas en diversas plataformas, alergenos recombinantes e IgE específicos de epítopes alergénicos que permiten un estudio puntual 1,2 y en consecuencia, un abordaje médico de precisión. Conocer las tecnologías actuales y sus aplicaciones en el diagnóstico molecular de la alergia es fundamental para tomar una decisión terapéutica basada en evidencias.

show abstract

Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches

Cited by 6 publications

References 55 publications

Determination of Specific IgG to Identify Possible Food Intolerance in Athletes Using ELISA

Determination of Specific IgG to Identify Possible Food Intolerance in Athletes Using ELISA

Fundamentals of Molecular Allergy: From Bench to Bedside

Capítulo 2. Diagnóstico molecular

Contact Info

Product

Resources

About