Allergen Immunotherapy Modulates Sensitivity of Treg Cells to Apoptosis in a Rat Model of Allergic Asthma

Datta, Ankur; Moitra, Saibal; Das, Prasanta Kumar; Mondal, Somnath; Faruk, Sk Md Omar; Hazra, Iman; Tripathi, Santanu Kumar; Chaudhuri, Swapna

doi:10.2217/imt-2017-0038

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…Critically, SATB1 but not FOXP3 expression in Tregs correlated with the alleviation of clinical symptoms [125••]. Reduced sensitivity to apoptosis in FOXP3 + Tregs is another proposed AIT-induced mechanism of propelling tolerance, as seen in a murine asthma model [106].…”

Section: Effect Of Ait On Regulatory T Cellsmentioning

confidence: 99%

Mechanisms of Allergen Immunotherapy in Allergic Rhinitis

Drazdauskaité

Layhadi

Shamji

2020

Curr Allergy Asthma Rep

110

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Purpose of Review Allergic rhinitis (AR) is a chronic inflammatory immunoglobulin (Ig) E-mediated disease of the nasal mucosa that can be triggered by the inhalation of seasonal or perennial allergens. Typical symptoms include sneezing, rhinorrhea, nasal itching, nasal congestion and symptoms of allergic conjunctivitis. AR affects a quarter of the population in the United States of America and Europe. Recent Findings AR has been shown to reduce work productivity in 36–59% of the patients with 20% reporting deteriorated job attendance. Moreover, 42% of children with AR report reduced at-school productivity and lower grades. Most importantly, AR impacts the patient’s quality of life, due to sleep deprivation. However, a proportion of patients fails to respond to conventional medication and opts for the allergen immunotherapy (AIT), which currently is the only disease-modifying therapeutic option. AIT can be administered by either subcutaneous (SCIT) or sublingual (SLIT) route. Both routes of administration are safe, effective, and can lead to tolerance lasting years after treatment cessation. Both innate and adaptive immune responses that contribute to allergic inflammation are suppressed by AIT. Innate responses are ameliorated by reducing local mast cell, basophil, eosinophil, and circulating group 2 innate lymphoid cell frequencies which is accompanied by decreased basophil sensitivity. Induction of allergen-specific blocking antibodies, immunosuppressive cytokines, and regulatory T and B cell phenotypes are key pro-tolerogenic adaptive immune responses. Conclusion A comprehensive understanding of these mechanisms is necessary for optimal selection of AIT-responsive patients and monitoring treatment efficacy. Moreover, it could inspire novel and more efficient AIT approaches.

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Section: Effect Of Ait On Regulatory T Cellsmentioning

confidence: 99%

Mechanisms of Allergen Immunotherapy in Allergic Rhinitis

Drazdauskaité

Layhadi

Shamji

2020

Curr Allergy Asthma Rep

110

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“…The traditional Th1/Th2 imbalance theory cannot explain all the pathogenesis. Presently, a number of studies have focused on Treg cells to investigate the pathognesis ( 36 , 37 ). Our findings demonstrated that AOS treatment reduced the serum levels of Th1 and Th2 cytokines (Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Astragalus Oral Solution Ameliorates Allergic Asthma in Children by Regulating Relative Contents of CD4+CD25highCD127low Treg Cells

2018

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Objective: To explore the effects of Astragalus oral solution (AOS) on allergic asthma in children by investigating relative contents of CD4+CD25highCD127low Treg cells.Methods: The contents of Astragaloside A in AOS were detected by using HPLC. Eighty children with allergic asthma were recruited from February 2016 to June 2017, and randomly assigned into the control group (received placebo, 0.1% quinine chloride in deionized water, daily) and the AOS group (received 10 mL AOS daily). After 6-month treatment, therapeutic results were compared between the two groups. Serum levels of IL-10 and TGF-beta, Th1 cytokines (IL-2 and IFN-γ), and Th2 cytokines (IL-4 and IL-6) were measured by using ELISA kits. Relative contents of CD4+CD25highCD127low Treg cells were determined by using flow cytometry.Results: Astragaloside A was the main ingredient of AOS with 0.216 ± 0.027 mg/mL from six-batch samples. After 6-month therapy, the AOS group showed improved forced expiratory volume in 1 s (FEV1) and the Pediatric Asthma Quality of Life Questionnaire (PAQLQ) scores compared with the control group (P < 0.05). Serum level of IL-10 was higher and the levels of TGF-beta, Th1 cytokines (IL-2 and IFN-γ), and Th2 cytokines (IL-4 and IL-6) were lower in the AOS group than in the control group (P < 0.05). AOS treatment increased the percentage of gated CD4+ T cells, CD4+CD25+ T cells, CD4+CD25high Treg cells, CD4+CD25+FoxP3+ Treg cells and CD4+CD25highCD127low Treg cells when compared with the control group (P < 0.05).Conclusions: Astragaloside A was the main component of AOS, and AOS ameliorated allergic asthma in children by regulating relative contents of CD4+CD25highCD127low Treg cells.

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“…Tregs induced basophil activation through the release of IL-3. It has been suggested that Tregs might also promote tumor evasion by activating basophils to augment and sustain Th2 responses in TME by secreting IL-3 ( 215 ).…”

Section: Basophils and Pancreatic Cancermentioning

confidence: 99%