Allergen-Induced Depression of Neutrophil Chemotaxis in Atopic Individuals

Abstract

Shl f ts in human 1yr.iphocy te subpopulations have been observed after exercise,following injection of steroids or epinephrine t. diurnally. The present study evaluates the effect of a nutrltional stimulus on peripheral blood lymphocytes. Six fasting normal subjects were given a 'nilkshoke' supplying one-tliird of the estimnted daily cnloric requirement,with defined amounts of carbohydrntc,fet end protein. The bioloqical activity eluted post albumin. It was electrophoresed on an LI(B iaoelectrofocusinq column. A major peak of proliferative activity was found at p~-6.7*0.1.A s e w n d minor pa& of activity was found at p~-7.2*0.1.In one instance, when the major neak was assayed for its ability to initiate de n o w synthesis of IgG by tonsillar B lymphocytes, it was found to cause a twofold increase of precipitable l4c activity over control.The data suqgast that stimulation of h m peripheral blood T lymphocytes by antiqen results in production of two factors that can induce DNA synthesis in autoloqous or alloqenic B 1ymphocyteS. It appears that both of these factors are of molecular Weiqht qroater than 50,000. At least one of the factors increases IqG ayntheais in B cells derived from human tonsils, while the other was present in concentrations too low to study. There was a significant difference in the %RFC and TRFC when comparing the ATG and non ATG reclplents (P=<0.001 and P 4 . 0 0 5 respectively). We conclude that TRFC and %RFC levels of children undergoing dialysis are not abnormal and that ATG significantly lowers %RFC and TRFC durln the first month Tx, whereas routine imnunosuppression only Yowers the TRFC but to a significantly less degree than ATG. A 21 month old female was admitted to hospital because of recurrent skin and upper respiratory infections and chronic neutropenia of 3 months duration. Family history was unremarkable and past history was negative for any known cauaes of neutropenia. Physical examination revealed multiple skin abscesses and marked hepatoaplenomegaly. Absolute granulocyte count varied between 150 and 500/~1; segmented neutrophils were absent in the bone marrow. RBC and platelet count were normal. T CELL LEVELS IN PATIENTS ON DIALYSIS AND IN THE 728 ALLERGEN-INDUCED DEPRESSION OF NEUTROPHILImmunological evaluation revealed normal C3, C4 and total hemolytic complement. Serum inmunoglobulins demonstrated absent IgA, low IgG (170 and 120 mgldl) and elevated IgM (190 and 740 mg/dl) at 21 and 27 months. respectively. Isohemagglutinins were absent (blood type 0, Rh positive). Despite previous inmunization, polio antibody titers were <1:4 for all types, but an antibody response followed antigenic challenge with diphtheria-tetanus antigens. There was no response to skin tests with SKSD and monilial antigens. E-rosette forming lymphocytes were diminished (36% vs 60% control) and PHA-stimulation was minimal (1 to 2-fold vs 11 to 24). Staining for immunoglobulin bearing B-cells was variable: IgG-15% and 51%. IgA-13% and 1%, IgM-7% and 8%. at 21 and 27 months, respectively. Serum leukoaggl...