Allergen Profiles of Dog Hair and Dander, Body Fluids and Tissues as Defined by Immunoblotting

Spitzauer, Susanne; Rumpold, Helmut; Ebner, C.; Schweiger, Ch.; Valenta, Rudolf; Gabl, F; Anrather, Josef; Breitenbach, Michael; Scheiner, Otto; Kraft, D

doi:10.1159/000235399

Cited by 14 publications

(4 citation statements)

References 10 publications

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“…There may be several possible reasons for the observed heterogeneities of the extracts: (i) different starting material from which manufacturers extract allergens (hair, dander or/and epithelium) may contain different allergens [8,26], (ii) the levels of the major dog allergen Can f 1 may vary between dog breed and sex and between individual dogs of the same breed [27], (iii) different extraction techniques as well as the presence of stabilizers like glycerol and phenol might contribute to differences in extract composition [14,28]. …”

Section: Discussionmentioning

confidence: 99%

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Curin¹,

Reininger²,

Swoboda³

et al. 2010

Int Arch Allergy Immunol

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Background: Commercial skin prick test (SPT) extracts used for the diagnosis of dog allergy are prepared by extracting allergens from natural sources, e.g. dog hair and dander. Due to different starting material and extraction methods used, it is likely that extracts differ regarding their allergen contents. Methods: The total protein content and composition of dog SPT extracts from 5 European manufacturers were compared by silver-stained SDS-PAGE. Specific antibody probes were generated to detect major and minor allergens in each extract by immunoblotting. Additionally, sera of patients suffering from dog allergy were used to detect dog allergens in SPT extracts. Results: SPT extracts showed a 20-fold variation regarding the total protein content. The contents of the major dog allergen Can f 1 and of Can f 2 varied considerably between the extracts. In one of the extracts, neither Can f 1 nor Can f 2 could be detected by immunoblotting. The contents of the minor dog allergen Can f 3, albumin, also showed great variability. In one of the dog SPT extracts, the presence of human serum albumin (HSA) was detected with HSA-specific antibodies. Conclusion: The observed variability of commercial dog SPT extracts regarding their allergen contents likely has a negative influence on the accuracy of diagnosis of dog allergy.

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Section: Discussionmentioning

confidence: 99%

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Curin¹,

Reininger²,

Swoboda³

et al. 2010

Int Arch Allergy Immunol

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show abstract

“…A large population-based study in Germany involving children and adolescents reported an IgE-sensitization prevalence of 9.7% to dog dander [ 55 ]. Dog-allergic patients reacted with more than 10 different proteins in the extracts of hair, dander, urine, skin, saliva, and serum [ 49 , 59 ]. The major dog allergen Can f 1 is a lipocalin with IgE prevalence ranging from 49 – 75% depending on the studied population [ 13 , 35 , 42 ].…”

Section: Dogmentioning

confidence: 99%

Allergy to pets and new allergies to uncommon pets

Curin¹,

Hilger²

2017

ALS

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Abstract. Animal dander is an important source of respiratory allergens, and sensitization to allergens from cat and/or dog during childhood represents a risk factor for the development of asthma and rhinitis later in life. The identification and characterization of allergenic components is crucial to improve diagnosis and therapy in patients with allergy to pets. Allergens from furry animals belong to a restricted number of protein families, a large majority are lipocalins or albumins, some are secretoglobins or latherins. Animal dander contains cross-reactive molecules and current efforts aim at defining species-specific allergens that have a high diagnostic sensitivity. Component-resolved diagnosis allows to discriminate genuine sensitization from cross-sensitization. This review contains a detailed description of allergenic components of cat, dog, horse, and small mammalian pets. Sensitizations to exotic pets, a newly emerging issue, are also discussed.

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“…These antigens are designated as Can f 1 and Can f 2 with molecular masses of 23 kd and 19 kd, respectively [11]. Like cat, major dog allergens can be found in hair, dander, and saliva, and are considered epithelial allergens [12]. Frequently, clinical observation has noted that many patients allergic to cats are also allergic to dogs.…”

Section: Allergensmentioning

confidence: 99%

Animal allergens and their control

Phipatanakul

2001

Curr Allergy Asthma Rep

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Animal allergens play a significant role in the pathogenesis of asthma and allergic rhinitis, and are potent causes of acute and chronic symptoms. Although cat and dog allergens are the most important, exposure to a wide variety of other furred animals is not uncommon. Recent reports state that 60% to 70% of households in the western world have at least one pet. Because of this significant exposure, hypersensitivity to animals has become increasingly important. This review focuses on the importance of animal allergens, concentrating on cat and dog allergens, but including others as well. It also discusses the pathogenesis, diagnosis, prevention, and management of animal allergy.

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Allergen Profiles of Dog Hair and Dander, Body Fluids and Tissues as Defined by Immunoblotting

Cited by 14 publications

References 10 publications

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Allergy to pets and new allergies to uncommon pets

Animal allergens and their control

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