Allergen-specific IgE and IgG4 patterns among patients with different allergic diseases

Smoldovskaya, Olga; Feyzkhanova, Guzel; Voloshin, S A; Arefieva, Alla; Чубарова, А. И.; Pavlushkina, Ludmila; Филатова, Т. А.; Antonova, E. E.; Timofeeva, Elena; Butvilovskaya, V. I.; Lysov, Yuri; Заседателев, А. С.; Rubina, A. Yu.

doi:10.1186/s40413-018-0220-5

Cited by 13 publications

(8 citation statements)

References 33 publications

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“…Our results are completely in agreement with earlier epidemiological studies which reported higher IgE levels associated with bronchial responsiveness, a major component of the asthma phenotype, rhinitis and dermatitis [ 31 – 33 ]. In recent years, a high serum levels of IgE is used to predict the development of asthma, independent of other allergic factors.…”

Section: Discussionsupporting

confidence: 93%

Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

Qurashi

Shah

Bhat

et al. 2021

Allergy Asthma Clin Immunol

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Objectives Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies. Methods A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR–RFLP methods. Results Statistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics. Conclusion Our study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.

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Section: Discussionsupporting

confidence: 93%

Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

Qurashi

Shah

Bhat

et al. 2021

Allergy Asthma Clin Immunol

View full text Add to dashboard Cite

show abstract

“…The results showed that serological IgG and IgE as well as IgG4 and IgE titers of an allergic patient had a significantly stronger positive correlation compared with the control subject (Fisher’s Z transformation, p < 0.05). While the mechanisms of IgGs involvement in allergic responses is still unclear, our finding is in accordance with previous reports suggesting that the levels of IgG4 increased with the increase in IgE in the allergic patients’ sera [26,27].…”

Section: Resultssupporting

confidence: 93%

Fabrication of Microarrays for the Analysis of Serological Antibody Isotypes against Food Antigens

Ryu

Kim

Song

et al. 2019

Sensors

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Food intolerance is delayed adverse food reactions which follow consumption of specific foods. The underlying mechanisms are not well understood, but food intolerance is often considered as a type 2 hypersensitivity reaction mediated by immunoglobulin G (IgG) antibody. To understand the causes of food intolerance, it is important to investigate sensitization patterns of food-specific IgGs (sIgG) in relation to dietary patterns and physical conditions. Conventional approaches to measure serological IgGs often require large volumes of serum, thus are not suitable for highly multiplexed assays. To overcome this impracticality, we developed a highly sensitive method to screen the sIgGs and other antibody isotypes against 66 antigens with minimal amount of serums. We prepared a microarray by immobilizing food antigens on activated glass slides. Human sera and their dietary information were obtained from 30 subjects. Aliquots (200 nl) of sera were analyzed against 66 food antigens in parallel. sIgG levels were determined and analyzed in relation to subjects’ dietary patterns. The levels of antibody isotypes were also examined to understand the relationship between allergy and food intolerance. The developed microarray showed exceptional performances in antibody screening and demonstrated the potential to be used as an automated assay system.

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“…There are four subclasses of IgG (IgG1, IgG2, IgG3 and IgG4). The inhaled allergens were strongly associated with serum IgG4 response [ 33 ]. Production of IgG4 in allergen-specific immunotherapy (AIT) is an immunological hallmark for successful tolerance establishment [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Allergic rhinitis, allergic contact dermatitis and disease comorbidity belong to separate entities with distinct composition of T-cell subsets, cytokines, immunoglobulins and autoantibodies

Chai

Zhang

Lin

et al. 2022

Allergy Asthma Clin Immunol

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Background Allergic rhinitis (AR) and allergic contact dermatitis (ACD) are prevalent allergic diseases and have significant impacts on patients’ daily life. Despite many studies on AR or ACD have been conducted separately, little is known about the immune responses in patients of AR combined with ACD and the interplay between AR and ACD. Our study compared various aspects of immune elements in patients with AR or/and ACD, aiming to characterize the immune responses in AR, ACD, and AR combined with ACD. Methods A total of 57 patients diagnosed with AR or/and ACD and 28 healthy volunteers were included. AR patients were further divided into seasonal AR (SAR) and perennial AR (PAR). All subjects’ blood samples were taken to assess the concentration of immunoglobulins, complement C3, C4, autoantibodies and cytokines in serum by immunoturbidimetry, ELISA or Luminex200 platform. Peripheral blood mononuclear cells (PBMCs) were subjected to the analysis of lymphocyte subpopulations by flow cytometry. Results It indicated that AR disease caused elevated levels of IgE, IgA, IgG, IgG4, as well as IL-4, IL-15, IL-8 and IL-6 in serum. AR patients possessed a decreased CD4/CD8 ratio and an increased proportion of memory CD4 + T-cell subset, with a skewed Th2 response and an enhanced CD8 + T-cell activation. Compared with patients with sole AR or ACD condition, AR + ACD patients presented with a significantly increased proportion of memory CD8 + T-cell subset and were prone to autoimmune disorders as indicated by the increased autoantibodies. The immune elements in patients with ACD only were least affected compared with those in other conditions. Additionally, seasonal or perennial AR patients exhibited different cytokine profiles and proportions of memory T-cell subsets. Conclusions In this study, we illuminated the respective characteristics of immune responses in AR, ACD, and AR combined with ACD. Meanwhile, we discovered that the PAR and SAR patients possessed different cytokine profiles and T-cell compartments. It suggested that these allergic conditions belong to different disease entities. Characterizing the detailed immune changes in these allergic diseases would help to develop proper treatments targeting particular immune elements in different allergic diseases.

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Allergen-specific IgE and IgG4 patterns among patients with different allergic diseases

Cited by 13 publications

References 33 publications

Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

Fabrication of Microarrays for the Analysis of Serological Antibody Isotypes against Food Antigens

Allergic rhinitis, allergic contact dermatitis and disease comorbidity belong to separate entities with distinct composition of T-cell subsets, cytokines, immunoglobulins and autoantibodies

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