Allergen-Specific Tolerance Induction: Of Mice and Men

Pfützner, Wolfgang

doi:10.1159/000375406

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…The induction of Treg cells and IL-10 has been shown to play an important role in maintaining peripheral T-cell tolerance, which is essential for normal immune responses and successful immunotherapy of allergic disorders [32,34,35]. We found that there were increased relative numbers of CD4+CD25+FOXP3+ cells in both the dLNs and the spleen in PLL-pAra h 2-treated mice, which were much higher than those of pAra h 2-treated mice.…”

Section: Discussionmentioning

confidence: 70%

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Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

Zhu

Yu²,

Niu³

et al. 2016

Int Arch Allergy Immunol

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Background: The prevalence of food allergy has been increasing, but treatment is very limited. DNA vaccination has been recognized as a promising method for the treatment of allergic diseases; however, poor immunogenicity has hindered its application. Methods: BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2 (pAra h 2) or pAra h 2 pretreated with poly-L- lysine (PLL) before or after sensitization with Ara h 2 protein. Ara h 2-specific antibodies were measured by ELISA. CD207+ dendritic cells (DCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization, and cytokine production in splenocytes was also analyzed. Results: In the prophylactic study, pretreatment with pAra h 2 or PLL-pAra h 2 resulted in lower levels of Ara h 2-specific IgG1, IgG2a, and IgE after sensitization with Ara h 2 protein, and mice in the PLL-pAra h 2 group had a significantly lower level of antibodies than those in the pAra h 2 group. In the treatment study, intradermal injection with pAra h 2 or PLL-pAra h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies, and PLL- pAra h 2 had stronger effects than pAra h 2. There were increased numbers of CD207+ DCs and Treg cells in the mice receiving intradermal injection with PLL-pAra h 2, and splenocytes from PLL-pAra h 2-treated mice secreted increased levels of IFN-γ and IL-10. Conclusions: Modification of pAra h 2 with PLL improved its prophylactic and therapeutic effects in peanut-allergic mice.

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Section: Discussionmentioning

confidence: 70%

“…Shifting from a Th2-type response towards a Th1-biased response has been demonstrated in anti-allergy DNA vaccine studies [32]. Most of the previous articles showed exaggerated Th1 responses after allergen challenge in sensitized mice [14,33].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

Zhu

Yu²,

Niu³

et al. 2016

Int Arch Allergy Immunol

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“…Immunotherapy induces an early and transient increase in allergen-specific regulatory T cells, which deviate Th2 responses to Th1 responses. Moreover, it can increase the level of allergen-specific IgG4 antibodies and reduce mast cell and basophil activation and mediator release [4, [23][24][25] . It has not been clearly established whether the clinical tolerance induced by OIT resembles natural tolerance or not [25] .…”

Section: Immunotherapy Modalities In Famentioning

confidence: 99%

Oral and Sublingual Immunotherapy: Potential Causes for Eosinophilic Gastrointestinal Disorders?

Babaie

Mesdaghi

Nishino

et al. 2017

Int Arch Allergy Immunol

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Food allergy is a common health problem worldwide, with increasing prevalence during recent decades. The only approved treatments for food allergy are food avoidance and administration of emergency medications in case of accidental exposure, which negatively affects patients' quality of life, so new treatments are highly desirable. Different food immunotherapy modalities have recently been used, with variable success rates in the induction of desensitization and tolerance, and different numbers and types of adverse reactions. Adverse reactions, especially intolerable gastrointestinal symptoms, are the most important causes of immunotherapy withdrawal. Eosinophilic esophagitis has been reported as a complication of milk, egg, and peanut oral immunotherapies and sublingual immunotherapy for respiratory allergies, but not for food allergies. Eosinophilic gastritis and eosinophilic colitis also rarely happened following egg and milk oral immunotherapies. The patients undergoing oral and sublingual immunotherapies should be closely followed up for a long time, and those with gastrointestinal symptoms should be evaluated by endoscopy of the gastrointestinal tract. These complications are usually reversible after early diagnosis and stopping the immunotherapy protocol.

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Allergen-Specific Tolerance Induction: Of Mice and Men

Cited by 2 publications

References 21 publications

Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

Oral and Sublingual Immunotherapy: Potential Causes for Eosinophilic Gastrointestinal Disorders?

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