Allergenic relevance of nonspecific lipid transfer proteins 2: Identification and characterization of Api g 6 from celery tuber as representative of a novel IgE-binding protein family

Vejvar, Eva; Himly, Martin; Briza, Peter; Eichhorn, Stephanie Pauline; Ebner, Christof; Hemmer, Wolfgang; Ferreira, Fátima; Gadermaier, Gabriele

doi:10.1002/mnfr.201300085

Cited by 33 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the recent past, nsLTPs have been identified from celery. While the nsLTP type 1 is expressed in the stalks, the nsLTP type 2 is found in the tuber . Only limited IgE cross‐reactivity is observed between those two different proteins.…”

Section: Major and Relevant Minor Allergenic Moleculesmentioning

confidence: 99%

See 1 more Smart Citation

EAACI Molecular Allergology User's Guide

Matricardi

Kleine-Tebbe²,

Hoffmann

et al. 2016

Pediatric Allergy Immunology

740

870

View full text Add to dashboard Cite

The availability of allergen molecules ('components') from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled 'component-resolved diagnosis' (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology User's Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, milk, egg, meat, fish, and shellfish), inhalants (pollen, mold spores, mites, and animal dander), and Hymenoptera venom. Diagnostic algorithms and short case histories provide useful information for the clinical workup of allergic individuals targeted for CRD. Part C covers protein families containing ubiquitous, highly cross-reactive panallergens from plant (lipid transfer proteins, polcalcins, PR-10, profilins) and animal sources (lipocalins, parvalbumins, serum albumins, tropomyosins) and explains their diagnostic and clinical utility. Part D lists 100 important allergen molecules. In conclusion, IgE-mediated reactions and allergic diseases, including allergic rhinoconjunctivitis, asthma, food reactions, and insect sting reactions, are discussed from a novel molecular perspective. The EAACI MAUG documents the rapid progression of molecular allergology from basic research to its integration into clinical practice, a quantum leap in the management of allergic patients.

show abstract

Section: Major and Relevant Minor Allergenic Moleculesmentioning

confidence: 99%

“…The culprit cross‐reactive allergens between mugwort and celeriac have not been identified so far. The clinical significance of the nsLTPs derived either from celeriac tuber (Api g 6) or from celery stalk (Api g 2) has not been confirmed to date .…”

Section: Clinical Relevance Diagnosis and Managementmentioning

confidence: 99%

EAACI Molecular Allergology User's Guide

Matricardi

Kleine-Tebbe²,

Hoffmann

et al. 2016

Pediatric Allergy Immunology

740

870

View full text Add to dashboard Cite

show abstract

“…Resistance to proteases throughout the gastrointestinal tract could represent one of the primary aspects to exert biological activities (de Lumen, 2005;Kannan, Hettiarachchy, Lay, & Liyanage, 2010;Vejvar et al, 2013).…”

Section: Food Chemistrymentioning

confidence: 99%

Isolation and Characterization of Wheat Derived Nonspecific Lipid Transfer Protein 2 (nsLTP2)

Bosi

Fiori

Dinelli

et al. 2018

Journal of Food Science

View full text Add to dashboard Cite

Numerous studies support the protective role of bioactive peptides against cardiovascular diseases. Cereals represent the primary source of carbohydrates, but they also contain substantial amounts of proteins, therefore representing a potential dietary source of bioactive peptides with nutraceutical activities. The analysis of wheat extracts purified by chromatographic techniques by means of HPLC-UV/nanoLC-nanoESI-QTOF allowed the identification of a signal of about 7 kDa which, following data base searches, was ascribed to a nonspecific lipid-transfer protein (nsLTP) type 2 from Triticum aestivum (sequence coverage of 92%). For the first time nsLTP2 biological activities have been investigated. In particular, in experiments with human umbilical vein endothelial cells (HUVEC), nsLTP2 displayed antioxidant and cytoprotective activities, being able to significantly decrease reactive oxygen species (ROS) levels and to reduce lactate dehydrogenase (LDH) release, generated following oxidative (hydrogen peroxide) and inflammatory (tumor necrosis factor α, interleukin-1β, and lipopolysaccharide) stimulation. The obtained promising results suggest potential protective role of nsLTP2 in vascular diseases prevention. PRACTICAL APPLICATION: nsLTP 2 peptide is resistant to proteases throughout the gastrointestinal tract and exerts antioxidant and cytoprotective activities. These characteristics could be exploited in vascular diseases prevention.

show abstract

“…Api g 5 is a flavin adenine dinucleotide (FAD) containing oxidase and contains N-glycans, which account for the majority of its IgE binding capacity [ 43 ]. Api g 2 and Api g 6 are both nsLTPs, differently expressed throughout the plant tissue [ 44 , 45 ]. So far, little is known about their relevance as major or minor allergens.…”

Section: Food Allergen Panels For Crdmentioning

confidence: 99%

Applications of Molecular Diagnostic Testing in Food Allergy

Hoffmann‐Sommergruber

Pfeifer

Bublin

2015

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

IgE-mediated food allergy is a relevant health problem inducing symptoms ranging from mild local reactions up to severe life-threatening situations. Currently, no immunotherapy is available and avoidance of the incriminating food is the method of choice. Therefore, reliable diagnostic tools to formulate dietary recommendations and to avoid unnecessary exclusion diets for the individual patient are urgently needed. This review provides an update on the current knowledge on food allergens and their application in various diagnostic approaches such as skin prick test, basophil activation test, and serum IgE testing. Furthermore, these new approaches are discussed and compared to conventional extract-based assays and correlated to the gold standard of food allergy diagnosis, the double-blind placebo-controlled food challenge. Finally, the application of food allergens for preventive measurements such as allergen detection assays and the determination of threshold levels for allergen levels are discussed.

show abstract

Allergenic relevance of nonspecific lipid transfer proteins 2: Identification and characterization of Api g 6 from celery tuber as representative of a novel IgE-binding protein family

Cited by 33 publications

References 46 publications

EAACI Molecular Allergology User's Guide

EAACI Molecular Allergology User's Guide

Isolation and Characterization of Wheat Derived Nonspecific Lipid Transfer Protein 2 (nsLTP2)

Applications of Molecular Diagnostic Testing in Food Allergy

Contact Info

Product

Resources

About