Allergic Sensitization Increases Contractile Responses to 5-HT in Guinea Pig Aorta

Campos-Bedolla, Patricia; De-La-Cruz-Negrete, R; Vargas, Mario H.; TORREJÓN-GONZÁLEZ, E; Mejía-Mendoza, D; Islas-Hernandez, Azul; Segura-Medina, Patricia; Córdoba-Rodríguez, Guadalupe; Orozco‐Suárez, Sandra; Arreola-Ramírez, José Luis

doi:10.33549/physiolres.934128

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Here, we found 23.1% contraction (at 10 −5 M; Fig. 2D ) in Habu snakes, which contrasts with previously reported values for thoracic aortic contraction in other species; specifically, 74% in rabbits [ 32 ], 120% in rats [ 2 ], and 47% in guinea pigs [ 4 ]. Secondly, we found a contractile response to ACh, as opposed to the relaxation response that has been reported in other snakes [ 7 , 18 ].…”

contrasting

confidence: 99%

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

OOTAWA,

WU,

SEKIO

et al. 2024

The Journal of Veterinary Medical Science

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Habu snakes ( Protobothrops flavoviridis ) are pit vipers found in the geographically adjacent but ecologically divergent islands of Tokunoshima and Amami-Oshima in southwestern Japan. Abiotic factors can cause variation in animal populations between the two islands, and Habu snakes may show such intraspecific physiological variation. We therefore evaluated the vasoreactivity in aortas isolated from the Habu of both islands. Tokunoshima Habu showed significantly greater contractile responses to angiotensin (Ang) II, acetylcholine (ACh) and noradrenaline, and significantly higher affinities (pEC 50 ) for Ang II and ACh, than Amami-Oshima Habu. ACh caused contractions in aortas from both populations, a finding previously unreported in snakes. Our findings indicate that vasoreactivity may differ between Tokunoshima and Amami-Oshima Habu.

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contrasting

confidence: 99%

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

OOTAWA,

WU,

SEKIO

et al. 2024

The Journal of Veterinary Medical Science

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“…Sensitization caused a statistically signi cant increase in the contractile response to 5-HT, a nding that our research group has already reported (Campos-Bedolla et al 2020). In the present study, we could not nd differences between aortas from control and sensitized animals regarding protein expression of the two major contraction-producing 5-HT 2 receptors, i.e., 5-HT 2A and 5-HT 2C, so an increased expression of these receptors after sensitization was discarded.…”

Section: Introductionsupporting

confidence: 76%

“…Thus, abnormal responsiveness to 5-HT induced by allergy may be the link between asthma and cardiovascular disorders. In a previous study, we found that aorta rings from guinea pigs sensitized to ovalbumin (OVA) had increased contractile responses to 5-HT compared with aortas from non-sensitized animals (Campos-Bedolla et al, 2020). To better understand the causes of this allergy-induced increased vascular responses to 5-HT, in the present study, we explored the role of the 5-HT 2 receptors family.…”

Section: Introductionmentioning

confidence: 89%

Role of 5-HT2 Receptors Family in the Allergy-Induced Increased Aorta Contractile Responses to 5-HT

Campos-Bedolla¹,

TORREJÓN-GONZÁLEZ²,

Mendoza-Mejía³

et al. 2021

Preprint

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Asthma poses an increased risk for cardiovascular disorders, suggesting that allergy, which is an underlying asthma process, causes atypical functioning of organs other than the lungs. A previous study in a guinea-pig asthma model, we concluded that allergic sensitization increased aorta contractile responses to 5-HT. To further characterize these responses, here, we explored the role of the 5-HT2 receptors family. We found that TCB-2 (5-HT2A agonist) and WAY161503 (5-HT2C agonist) induced aorta contractions resembling those elicited by 5-HT but less intense (~43% and ~25%, respectively). In these experiments, aortas from sensitized guinea pigs showed increased contractions to TCB-2 but not to WAY161503. In turn, MDL 100907 (5-HT2A antagonist) and RS-102221 (5-HT2C antagonist) caused a notably and mild reduction of the 5-HT-induced contractions, respectively, with no differences seen between sensitized and non-sensitized tissues. BW723C86 (5-HT2B agonist) did not induce contractile responses, and RS-127445 (5-HT2B antagonist) did not modify the contractile responses to 5-HT. In non-sensitized aortas, the pattern of protein expression of receptors was 5HT2B>5-HT2A=5-HT2C, which did not change in sensitized animals. In conclusion, we found that allergic sensitization increased the aorta contractile responses to 5-HT, partly mediated by enhanced responses of 5-HT2A receptors, which was unrelated to changes in these receptors' expression.

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Itch and Cough – Similar Role of Sensory Nerves in Their Pathogenesis

Pecova

Kocan²,

Vyšehradský³

et al. 2020

Physiol Res

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Itch is the most common chief complaint in patients visiting dermatology clinics and is analogous to cough and also sneeze of the lower and upper respiratory tract, all three of which are host actions trying to clear noxious stimuli. The pathomechanisms of these symptoms are not completely determined. The itch can originate from a variety of etiologies. Itch originates following the activation of peripheral sensory nerve endings following damage or exposure to inflammatory mediators. More than one sensory nerve subtype is thought to subserve pruriceptive itch which includes both unmyelinated C-fibers and thinly myelinated Aδ nerve fibers. There are a lot of mediators capable of stimulating these afferent nerves leading to itch. Cough and itch pathways are mediated by small-diameter sensory fibers. These cough and itch sensory fibers release neuropeptides upon activation, which leads to inflammation of the nerves. The inflammation is involved in the development of chronic conditions of itch and cough. The aim of this review is to point out the role of sensory nerves in the pathogenesis of cough and itching. The common aspects of itch and cough could lead to new thoughts and perspectives in both fields.

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Allergic Sensitization Increases Contractile Responses to 5-HT in Guinea Pig Aorta

Cited by 4 publications

References 25 publications

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

Role of 5-HT2 Receptors Family in the Allergy-Induced Increased Aorta Contractile Responses to 5-HT

Itch and Cough – Similar Role of Sensory Nerves in Their Pathogenesis

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