Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

Zakzuk, Josefina; Lozano, Ana; Caraballo, Luis

doi:10.3389/falgy.2021.695262

Cited by 5 publications

(5 citation statements)

References 94 publications

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“…In the present study, we assessed the potential application of GST as a broad-spectrum vaccine antigen against avian mites. Previously, GSTs were identified and characterized as molecules involved in acaricide detoxification in PRMs [ 44 ]. However, PRM GSTs have not been assessed as vaccine antigens.…”

Section: Discussionmentioning

confidence: 99%

“…rGSTs of PRMs catalyze the conjugation of GSH to 1-chloro-2,4-dinitrobenzene (CDNB) substrate [ 44 ]. In this study, we measured enzyme activity at different concentrations of each rGST in the presence of a constant concentration of GSH and the CDNB substrate.…”

Section: Methodsmentioning

confidence: 99%

“…GSTs are present in various tissues and catalyze a variety of compounds in the presence of glutathione (GSH), resulting in fewer harmful compounds [ 42 ]. GSTs from several species of parasites and pests, such as house dust mites, parasitic nematodes, and cockroaches, cause allergenic reactions in vertebrates and humans [ 43 , 44 ]. Alpha, mu, theta, and sigma classes of GSTs have been identified in chickens, and these heterologously expressed proteins have unique properties as avian GSTs [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

Win,

Seo,

Horio

et al. 2024

Vaccines

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Poultry red mites (Dermanyssus gallinae, PRMs), tropical fowl mites (Ornithonyssus bursa, TFMs), and northern fowl mites (O. sylviarum, NFMs) are blood-feeding pests that debilitate poultry worldwide. Glutathione S-transferase (GST) plays an important role in the detoxification and drug metabolism of mites. However, research on avian mite GSTs as vaccine antigens is still lacking. Therefore, we aimed to evaluate the potential of avian mite GSTs for vaccine development. We identified GST genes from TFMs and NFMs. We prepared recombinant GST (rGST) from TFMs, NFMs, and PRMs, and assessed their protein functions. Moreover, we evaluated the cross-reactivity and acaricidal effect of immune plasma against each rGST on TFMs, NFMs, and PRMs. The deduced amino acid sequences of GSTs from TFMs and NFMs were 80% similar to those of the PRMs. The rGSTs exhibited catalytic activity in conjugating glutathione to the 1-chloro-2,4-dinitrobenzene substrate. Immune plasma against each rGST showed cross-reactivity with rGST from different mite species. Moreover, the survival rate of PRMs fed with immune plasma against the rGST of TFMs and NFMs was significantly lower than that of the control plasma. These results demonstrate the potential application of GST as an antigen for the development of a broad-spectrum vaccine against avian mites.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

Win,

Seo,

Horio

et al. 2024

Vaccines

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“…The use of species‐specific components might be necessary in places like the tropics where co‐exposure to both genera is common. Tropomyosin is the main cause of cross‐reactivity among mites, cockroaches, shellfish and helminths (e.g., Ascaris lumbricoides), but Glutathione‐S‐Transferase may also be involved 736 (Figure 58).…”

Section: B04 – Dust Mite Allergymentioning

confidence: 99%

EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

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119

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Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE‐mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE‐mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well‐defined, highly pure molecules for component‐resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the “EAACI Molecular Allergology User's Guide” (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state‐of‐the‐art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.

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“…Antioxidants, such as glutathione S-transferases (GSTs), are detoxification enzymes that play an important role in protection against the free radicals generated by host immune cells, decreasing pro-inflammatory responses both in DCs and macrophages (106)(107)(108)(109)144).…”

Section: Helminth Secreted Proteinsmentioning

confidence: 99%

How to train your myeloid cells: a way forward for helminth vaccines?

Doolan,

Putananickal,

Tritten

et al. 2023

Front. Immunol.

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Soil-transmitted helminths affect approximately 1.5 billion people worldwide. However, as no vaccine is currently available for humans, the current strategy for elimination as a public health problem relies on preventive chemotherapy. Despite more than 20 years of intense research effort, the development of human helminth vaccines (HHVs) has not yet come to fruition. Current vaccine development focuses on peptide antigens that trigger strong humoral immunity, with the goal of generating neutralizing antibodies against key parasite molecules. Notably, this approach aims to reduce the pathology of infection, not worm burden, with only partial protection observed in laboratory models. In addition to the typical translational hurdles that vaccines struggle to overcome, HHVs face several challenges (1): helminth infections have been associated with poor vaccine responses in endemic countries, probably due to the strong immunomodulation caused by these parasites, and (2) the target population displays pre-existing type 2 immune responses to helminth products, increasing the likelihood of adverse events such as allergy or anaphylaxis. We argue that such traditional vaccines are unlikely to be successful on their own and that, based on laboratory models, mucosal and cellular-based vaccines could be a way to move forward in the fight against helminth infection. Here, we review the evidence for the role of innate immune cells, specifically the myeloid compartment, in controlling helminth infections. We explore how the parasite may reprogram myeloid cells to avoid killing, notably using excretory/secretory (ES) proteins and extracellular vesicles (EVs). Finally, learning from the field of tuberculosis, we will discuss how anti-helminth innate memory could be harnessed in a mucosal-trained immunity-based vaccine.

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Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

Cited by 5 publications

References 94 publications

In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

EAACIMolecular Allergology User's Guide 2.0

How to train your myeloid cells: a way forward for helminth vaccines?

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