Alleviating Asthma-Induced Mitochondrial Dysfunction and Oxidative Stress: Lactobacillus rhamnosus Intervention via Notch1 Signaling Pathway

Abstract

Immune system disorders play a crucial role in asthma. Probiotics can be beneficial in maintaining a healthy balance of gut bacteria and improving metabolism. Lactobacillus rhamnosus (L. rhamnosus) is a gram-positive probiotic found primarily in the intestines of humans and animals. However, the mechanism by which L. rhamnosus treats asthma is unknown. Here, we confirmed that L. rhamnosus had a protective effect on the lung tissues of asthmatic mice. In addition, L. rhamnosus significantly modulated the balance of T cell subpopulations and promoted the expression of Treg cells, increasing the proportion of inhibited Th17 and restoring immune function. Mechanistically, L. rhamnosus inhibited the activation of NOD-like receptor heat protein domain-related protein 3 (NLRP3) inflammatory vesicles caused by asthma-induced oxidative stress and mitochondrial dysfunction. In conclusion, L. rhamnosus ameliorated asthma by mediating Notch1 signaling pathway-induced NLRP3 inflammasome activation and Dynamin-related protein 1 (Drp1) translational activation. In words, our study provides a theoretical basis for the use of probiotics, represented by L. rhamnosus, as effective agents for the prevention and treatment of asthma.