Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production

Towards a hybrid model-driven platform based on flux balance analysis and a machine learning pipeline for biosystem design

“…The detailed methods for plasmid and strain construction can be referred to published reports [ 26 , 27 ]. In brief, the deletion strains were constructed through CRISPR/Cas9-mediated genome editing [ 28 ].…”