Alleviating ischemia-reperfusion injury in aged rat liver by induction of heme oxygenase-1

Wang, X.H.; Wang, K.; Zhang, F.; Li, X.C.; Qian, Xiaofeng; Cheng, Feng; Li, G.Q.; Fan, Ye-Dong

doi:10.1016/j.transproceed.2004.10.066

Cited by 21 publications

(17 citation statements)

References 35 publications

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“…Although the majority of in vitro and in vivo studies conducted have attributed the protective effect of rutin to its chemical reactivity toward free radicals and its capacity to prevent the oxidation of important intracellular components, Motterlini et al (34) recently suggested a possible novel aspect in the mode of action of polyphenol compounds; that is, the ultimate stimulation of the HO-1 pathway is likely to account for the established and powerful antioxidant/anti-inflammatory properties of these polyphenols (42).…”

Section: Discussionmentioning

confidence: 99%

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

Acquaviva¹,

Lanteri²,

G³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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following liver ischemia results in oxidative stress leading to liver injury. The aim of this study was to investigate the combined effects of two antioxidant agents, rutin and L-arginine, in rat liver ischemia-reperfusion (I/R). Male Wistar rats were divided into five groups: 1) sham operated, 2) I/R, 3) I/R ϩ rutin, 4) I/R ϩ L-arginine, and 5) I/R ϩ rutin ϩ L-arginine. Plasmatic and hepatic levels of alanine transaminase (ALT), aspartate transaminase (AST), lipid peroxides (LOOH), and thiol groups (RSH) were examined, as well as DNA fragmentation and liver histopathology. Furthermore, to elucidate the pathophysiological processes involved in the antioxidant mechanism(s) of rutin and L-arginine, we assessed the expression of inducible (iNOS) and endothelial nitric oxide synthase (eNOS) isoforms and heme oxygenase-1 (HO-1), both playing key roles in the biochemical cascade of liver injury. Significant increase in plasmatic ALT and AST activities were observed in untreated I/R rats compared with sham-operated animals, whereas treatment with rutin or L-arginine in I/R rats reduced hepatic damage. Interestingly, combined therapy with rutin and Larginine resulted in a further reduction of plasmatic ALT and AST activities compared with rutin or L-arginine alone. These results were further confirmed by the analysis of DNA fragmentation, LOOH,

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Section: Discussionmentioning

confidence: 99%

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

Acquaviva¹,

Lanteri²,

G³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Recent study has shown the critical importance of HO-1 expression in mediating antiapoptotic effects (Kim et al, 2006b). HO-1 overexpression could decrease apoptotic cell death by enhancing Bcl-2 and depressing caspase-3 expression (Wang et al, 2004). Induction of HO-1 by chemical inducers or selective overexpression is cytoprotective both in vitro and in vivo (Nakahira et al, 2003;Nath, 2006).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of naringenin-7-O-glucoside on doxorubicin-induced apoptosis in H9C2 cells

Han

Ren

Fan

et al. 2008

European Journal of Pharmacology

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Doxorubicin, a widely used chemotherapeutic agent, can give rise to severe cardiotoxicity by inducing cardiomyocyte apoptosis. Dracocephalum rupestre Hance, a Chinese traditional herb, has therapeutic potential for cardiovascular diseases. Naringenin-7-O-glucoside is the main active constituent of D. rupestre and there is increasing interest in its therapeutic applications. The aim of this study was to evaluate the effects of naringenin-7-O-glucoside on cardiomyocyte apoptosis induced by doxorubicin. Cell viability was detected by MTT assay. Naringenin-7-Oglucoside (10, 20, and 40 μM) significantly enhanced cardiomyocyte proliferation relative to that of doxorubicin. Furthermore, naringenin-7-Oglucoside increased the protein levels of heme oxygenase-1 (HO-1) and Bcl-2 in cardiomyocytes (as detected by Western blotting) and suppressed the mRNA expression of caspase-3 and caspase-9 (as detected by RT-PCR). These results suggest that naringenin-7-O-glucoside has protective effects against doxorubicin-induced apoptosis, effects which could underlie the use of naringenin-7-O-glucoside therapeutic agent for treating or preventing cardiomyopathy associated with doxorubicin.

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“…On the other hand, cells with compromised HO-1 expression had a decrease in stress defence [17]. HO-1 alleviates tissue injury not only by acting as an antioxidant, but also by modulating inflammatory immune reactions, and activating the anti-apoptotic process and other signal transduction pathways [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Haem oxygenase-1 gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury

Peng

Chen

et al. 2008

Clinical Science

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RGC (retinal ganglion cell) death following ischaemic insult is the major cause of a number of vision-threatening diseases, including glaucoma. The aim of the present study was to evaluate the role of HO-1 (haem oxygenase-1) in the retina against IR (ischaemia/reperfusion) injury. Adenovirus-mediated HO-1 gene transfer (Adv-HO-1) was carried out by injection into the vitreous body to induce HO-1 overexpression. At 3 weeks after transfection, levels of HO-1 expression, as measured by Western blot analysis, immunohistochemical staining and activity assay, were drastically up-regulated. Transient retinal ischaemia was induced by raising the intraocular pressure to 150 mmHg for 60 min. Untreated IR caused a significant decrease in RGC numbers at 3 and 7 days after reperfusion (76.1 and 67.2% of control eyes with sham IR respectively; P<0.001). Eyes pretreated with Adv-HO-1 had less RGC loss on day 3 and 7 following reperfusion compared with control eyes injected with Adv-GFP (adenovirus containing a gene for green fluorescent protein; 94.3 and 88.2% respectively; P=0.007 and 0.001). SnP (tin protoporphyrin), an HO-1 inhibitor, counteracted the effects of Adv-HO-1. In conclusion, these findings provide evidence that augmentation of HO-1 enzyme overexpression by intravitreal injection is able to protect RGCs against IR-induced damage.

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Alleviating ischemia-reperfusion injury in aged rat liver by induction of heme oxygenase-1

Cited by 21 publications

References 35 publications

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

Protective effects of naringenin-7-O-glucoside on doxorubicin-induced apoptosis in H9C2 cells

Haem oxygenase-1 gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury

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