Alleviating shoot tip necrosis during in vitro propagation of grape cv. Red Globe

Surakshitha, N.C.; Soorianathasundaram, K.; Ganga, M.; Raveendran, M.

doi:10.1016/j.scienta.2019.01.013

Cited by 24 publications

(22 citation statements)

References 29 publications

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“…Por otro lado, el tratamiento MS + 0,2 mg L -1 ANA obtuvo la menor cantidad de explantes muertos (14%), valor que se diferenció significativamente de los tratamientos restantes por ser del 33 y del 41% en los tratamientos MS + 1 mg L -1 BAP, y MS + 1 mg L -1 BAP + 0,2 mg L -1 ANA, respectivamente, y del 67% en el tratamiento testigo. Posiblemente, este alto valor de mortandad se desencadenó a partir de la composición del medio de cultivo ya que, al no tener reguladores de crecimiento, los explantos no lograron adquirir los nutrientes requeridos para optimizar su desarrollo y se necrosaron (Surakshita et al, 2019). En el caso de los tratamientos restantes, los resultados de estas variables pudieron deberse a que la baja producción de raíces impidió la adecuada absorción de los nutrientes proporcionados por los diferentes medios de cultivo y produjo la muerte de los tejidos.…”

Section: Resultados Y Discusiónunclassified

DPROPAGACIÓN IN VITRO DE Alstroemeria var. ‘FIESTA DE 15 INTA’ A PARTIR DEL CULTIVO DE MERISTEMAS DE RIZOMA

Tombion

Soto

Mori

et al. 2020

Chil. j. agric. anim. sci.

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Alstroemeria (Alstroemeriaceae) es un género nativo de Sudamérica. Sus especies, que se distribuyen desde Venezuela hasta Argentina y Chile, tienen alto impacto en el mercado de plantas ornamentales, debido al color y atractiva forma de sus flores. El cultivo in vitro de meristemas aumenta la eficiencia de la propagación de las plantas y su calidad, por contribuir con el saneamiento de enfermedades sistémicas. El presente estudio tuvo como objetivo desarrollar un protocolo eficiente de cultivo in vitro de meristemas de rizoma de Alstroemeria híbrida var. Fiesta de 15 INTA, a partir del agregado de reguladores de crecimiento bencilaminopurina (BAP) y ácido naftalenacético (ANA) a un medio de cultivo base. Se evaluó la cantidad total de plantas completas alcanzadas, producción de hojas, longitud aérea y radical, formación de callo, necrosis y mortandad de los explantos sembrados sobre MS; MS + 1 mg L-1 BAP; MS + 0,2 mg L-1 ANA; y MS + 0,2 mg L-1 ANA + 1 mg L-1 BAP. Se realizó un análisis de la varianza y la evaluación de diferencias entre medias por el test de Tukey. La suplementación de 0,2 mg L-1 ANA al medio MS es suficiente para el cultivo de meristemas de rizoma de Alstroemeria var. Fiesta de 15 INTA, ya que su empleo resultó en una mayor producción aérea y radical y, consecuentemente de plantas completas, además de un alto porcentaje de sobrevivencia de plantas luego de su transferencia al invernáculo. Palabras clave: Alstroemeria, cultivo de meristemas, micropropagación, reguladores de crecimiento, bencilaminopurina (BAP), ácido naftalenacético (ANA).

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Section: Resultados Y Discusiónunclassified

DPROPAGACIÓN IN VITRO DE Alstroemeria var. ‘FIESTA DE 15 INTA’ A PARTIR DEL CULTIVO DE MERISTEMAS DE RIZOMA

Tombion

Soto

Mori

et al. 2020

Chil. j. agric. anim. sci.

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“…Thomas ( 2000 ) observed that CK concentration had no signficant effect on STN. Surakshitha et al ( 2019 ) did not observe this effect in grape; instead, the level of STN depended on BA concentration. When BA concentration was increased from 8.9 µM (0% STN) to 17.8 µM, cane apple ( Arbutus unedo L.) cultures displayed 8.7% STN (Gomes et al 2010 ).…”

Section: Plant Growth Regulators Affect Stnmentioning

confidence: 90%

“…Frequent (shorter) subcultures. Use of half-strength MS rather than MS Surakshitha et al ( 2019 ) Only studies for which data or other evidence was provided are shown; all other studies that claimed to have observed STN, but did not provide evidence or show data are discussed only in the text. Studies for which no data exist to support the claim of STN are not included in this table, but are instead discussed in the main text.…”

Section: Shoot Tip Necrosis: Occurrence and Alleviationmentioning

confidence: 99%

“…Therefore, it is inconclusive to attribute the problem of STN exclusively to Ca 2+ deficiency while the role of Cl − is completely overlooked. Numerous examples of this inconclusiveness can be found in the literature (Barghchi and Alderson 1996 ; Piagnani et al 1996 ; Bairu et al 2009a , 2009b ; Ozgen et al 2011 ; Machado et al 2014 ; Poothong and Reed 2014 ; Surakshitha et al 2019 ). Nevertheless, it has recently been proved that Cl − (> 4.67 mM) has a positive effect on reducing STN symptoms in pistachio (Nezami-Alanagh et al 2019 ).…”

Section: Nutrient Deficienciesmentioning

confidence: 99%

“…A CK × Ca 2+ × interaction on STN was observed in grape ( Vitis vinifera L.) cv. Red Globe where supplementary CaCl 2 and H 3 BO 3 were needed to suppress STN, even after the level of BA had been optimized (Surakshitha et al 2019 ). Thomas ( 2000 ) observed that CK concentration had no signficant effect on STN.…”

Section: Plant Growth Regulators Affect Stnmentioning

confidence: 99%

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Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

Silva

Nezami-Alanagh

Barreal

et al. 2020

Planta

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Main conclusion Shoot tip necrosis is a physiological condition that negatively impacts the growth and development of in vitro plant shoot cultures across a wide range of species. Abstract Shoot tip necrosis is a physiological condition and disorder that can arise in plantlets or shoots in vitro that results in death of the shoot tip. This condition, which can spread basipetally and affect the emergence of axillary shoots from buds lower down the stem, is due to the cessation of apical dominance. STN can occur at both shoot multiplication and rooting stages. One of the most common factors that cause STN is nutrient deficiency or imbalance. Moreover, the presence or absence of plant growth regulators (auxins or cytokinins) at specific developmental stages may impact STN. The cytokinin to auxin ratio within an in vitro plant can be modified by varying the concentration of cytokinins used in the culture medium. The supply of nutrients to in vitro shoots or plantlets might also affect their hormonal balance, thus modifying the occurrence of STN. High relative humidity within culture vessels and hyperhydricity are associated with STN. An adequate supply of calcium as the divalent cation (Ca2+) can hinder STN by inhibiting the accumulation of phenolic compounds and thus programmed cell death. Moreover, the level of Ca2+ affects auxin transport and ethylene production, and higher ethylene production, which can occur as a result of high relative humidity in or poor ventilation of the in vitro culture vessel, induces STN. High relative humidity can decrease the mobility of Ca2+ within a plant, resulting in Ca2+ deficiency and STN. STN of in vitro shoots or plantlets can be halted or reversed by altering the basal medium, mainly the concentration of Ca2+, adjusting the levels of auxins or cytokinins, or modifying culture conditions. This review examines the literature related to STN, seeks to discover the associated factors and relations between them, proposes practical solutions, and attempts to better understand the mechanism(s) underlying this condition in vitro.

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Features of the Morphological Development of Grape Plants at the Stage of Shoot Rooting, Depending on the Concentration and Ratio of Macrosalts in the Nutrient Medium

Rebrov

2023

Lecture Notes in Networks and Systems

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Alleviating shoot tip necrosis during in vitro propagation of grape cv. Red Globe

Cited by 24 publications

References 29 publications

DPROPAGACIÓN IN VITRO DE Alstroemeria var. ‘FIESTA DE 15 INTA’ A PARTIR DEL CULTIVO DE MERISTEMAS DE RIZOMA

DPROPAGACIÓN IN VITRO DE Alstroemeria var. ‘FIESTA DE 15 INTA’ A PARTIR DEL CULTIVO DE MERISTEMAS DE RIZOMA

Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

Features of the Morphological Development of Grape Plants at the Stage of Shoot Rooting, Depending on the Concentration and Ratio of Macrosalts in the Nutrient Medium

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