Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

Lee, Joon-Kyoung; Choi, Eun Hye; Lee, Kwang‐Geun; Chun, Hyang Sook

doi:10.1016/j.lfs.2005.03.028

Cited by 34 publications

(20 citation statements)

References 36 publications

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“…Other studies based mainly on results of 32 P postlabeling experiments support our findings and have showed dependent formation of DNA adduct spots after exposure to AFB1 [41] and OTA . Moreover, the 8-hydroxy-2 -deoxyguanosine adduct has been reported to be formed through the phenolic radical of AFB1 [43] and OTA [44].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells

Golli-Bennour

Kouidhi

Bouslimi

et al. 2010

J Biochem & Molecular Tox

102

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Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are important food-borne mycotoxins that have been implicated in human health. In this study, independent and combinative toxicities of AFB1 and OTA were tested in cultured monkey kidney Vero cells. The experiments reported here were conducted to evaluate the effect of these toxins on cell viability followed by the determination of cell death pathways, using the quantification of DNA fragmentation and the expression of p53 and bcl-2 protein levels. Our results showed that AFB1 and OTA caused a marked decrease of cell viability in a dose-dependent manner. Under the same conditions, these mycotoxins increased fragmented DNA levels. In addition, p53 was activated in response to DNA damage and the expression of the antiapoptotic factor bcl-2 decreased significantly. According to these data, AFB1 and OTA seemed to be involved in an apoptotic process. Moreover, combined AFB1 and OTA induced all the toxicities observed with the mycotoxins separately. Therefore, this combination was classified as an additive response of the two mycotoxins.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells

Golli-Bennour

Kouidhi

Bouslimi

et al. 2010

J Biochem & Molecular Tox

102

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“…In addition to the genotoxic and mutagenic actions, accumulated evidence have shown that oxidative damage plays a crtical role in aflatoxin B 1 -induced cytotoxicity and carcinogenicity (Towner et al, 2003;Lee et al, 2005). The oxidative damage results from enhanced accumulation of ROS (such as O 2 -, H 2 O 2 and lipid hydroperoxides) during metabolic processing of AFB 1 by liver cytochrome P450 (Shen et al, 1996;1995).…”

Section: Introductionmentioning

confidence: 99%

Phenolic extract of Parkia biglobosa fruit pulp stalls aflatoxin B1 – mediated oxidative rout in the liver of male rats

Ajiboye

Adeleye

Salau

et al. 2014

Revista Brasileira de Farmacognosia

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Oxidative stress DNA fragmentation lipid peroxidation A B S T R A C TThe effect of phenolic extract of Parkia biglobosa (Jacq.) R. Br. ex G. Don, Fabaceae, pulp on aflatoxin B 1 induced oxidative imbalance in rat liver was evaluated. Thirty-five male rats were randomized into seven groups of five animals each. Rats in group A served as control and received vehicle for drug administration (0.5% DMSO) once daily at 24 h intervals for six weeks. Rats in groups B, D, E, F and G, received aflatoxin B 1 (167 μg/kg body weight) in 0.5% DMSO for three weeks, starting from the third week of the experimental period. Rats in Group C received 400 mg/kg bodyweight of the extract for six weeks, while groups D, E and F rats were treated with 100, 200 and 400 mg/kg bodyweight of the extract for six weeks respectively. Group G rats received 100 mg/kg body weight of vitamin C. Aflatoxin B 1 -mediated decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase were significantly attenuated. Aflatoxin B 1 mediated the elevation in malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl, and significantly lowered DNA fragmentation percentage.Overall, the phenolic extract of P. biglobosa pulp stalls aflatoxin B 1 -mediated oxidative rout by enhancing antioxidant enzyme activities leading to decreased lipid peroxidation, protein oxidation and DNA fragmentation.

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“…Evaluation of the lipid damage is based on measurement of Thiobarbituric acid reactive substances (TBARS) or MDA by the TBA test and conjugated dienes. AFB1 induced an increase in the TBARS concentration in the liver [74] or in human hepatoma cells [75]. The increase of the lipid peroxide synthesis is observed not only in the liver but also in the kidney and brain [7,35,41].…”

Section: Alatoxins and Oxidative Lipid Damagementioning

confidence: 99%

The Effect on Oxidative Stress of Aflatoxin and Protective Effect of Lycopene on Aflatoxin Damage

Yılmaz¹,

Kaya²,

Kisaçam³

2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Alatoxin (AF) is polysubstituted bifuranocoumarins that are secondary fungal metabolites produced by parasiticus/lavus group of the genus Aspergillus. AF is hepatotoxic, nephrotoxic, mutagenic, teratogenic, genotoxic, and immunotoxic, so the International Agency for Research on Cancer has classiied AF as class I human carcinogen. AF-mediated cell injury may be associated with the release of free radicals, and these radicals initiate lipid peroxidation and a damaging process in biological systems since all cell membranes contain the polyunsaturated faty acids (PUFAs), which are substrates for such a reaction. One of the causes for AF-induced toxicity is the oxidative stress, which leads to the improved generation of reactive oxygen species (ROS) and the oxidative DNA damage. Lycopene, a naturally occurring carotenoid, has drawn a particular atention in recent years because of its high antioxidant activity and free radical scavenging capacity and has been shown to be efective against oxidative stress due to AF. Lycopene blocks Phase 1 metabolic enzymes of AFB such as 3A4, 2A6, and 1A2.

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Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

Cited by 34 publications

References 36 publications

Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells

Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells

Phenolic extract of Parkia biglobosa fruit pulp stalls aflatoxin B1 – mediated oxidative rout in the liver of male rats

The Effect on Oxidative Stress of Aflatoxin and Protective Effect of Lycopene on Aflatoxin Damage

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