Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production

Jung, Moo Young; Jung, Hwi Min; Lee, Jinwon; Oh, Min Kyu

doi:10.1186/s13068-015-0290-3

Cited by 41 publications

(18 citation statements)

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“…Conversion of lignocellulosic waste to 2,3-BDO was introduced by Flickinger [ 11 ]. To date, Klebsiella pneumoniae , Serratia marcescens , Enterobacter aerogenes , Paenibacillus polymyxa, and Saccharomyces cerevisae have shown potential to produce 2,3-BDO [ 12 , 13 ], but the Klebsiella family has a higher capability than the others [ 14 , 15 ]. Lee and Maddox [ 16 ] used whey permeate K. pneumoniae immobilized in calcium alginate to produce 2,3-BDO with the productivity of 2–3 g/L/ h −1 .…”

Section: Introductionmentioning

confidence: 99%

Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis

Harvianto

Haider

Jiang

et al. 2018

Biotechnol Biofuels

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Background2,3-Butanediol (2,3-BDO) is a synthetic chemical compound that also can be produced by biomass fermentation, which is gaining share in the global market as an intermediate product for numerous applications, i.e. as liquid fuel or fuel additive. Several metabolic engineering fermentation strategies to enhance the production of 2,3-BDO were developed. However, the recovery of 2,3-BDO from its fermentation broth remains a challenge due to its low concentration and its solubility in water and other components. Thus, a cost-effective recovery process is required to deliver the required purity of 2,3-BDO. This paper presents a new process development and techno-economic analysis for 2,3-BDO purification from a fermentation broth.ResultsConventional distillation and hybrid extraction-distillation (HED) processes are proposed in this study with detailed optimization and economic analysis. Particularly, a systematic solvent selection method was successfully implemented to determine a good solvent for the proposed HED configuration based on numerous experimental data obtained with each solvent candidate. NRTL and UNIQUAC property methods were evaluated to obtain binary interaction parameters of 2,3-BDO through rigorous Aspen Plus regression and validated using experimental data. Total annual cost (TAC)-based optimization was performed for each proposed configuration. Even though the HED configuration required 9.5% higher capital cost than conventional distillation, placing an extraction column before the distillation column was effective in removing water from the fermentation broth and significantly improved the overall process economics.ConclusionsOleyl alcohol was found to be the most suitable solvent for the HED of 2,3-BDO due to its high distribution coefficient and high selectivity. The proposed HED drastically reduced reboiler duty consumption and TAC by up to 54.8 and 25.8%, respectively. The proposed design is expected to be used for the commercial scale of 2,3-BDO production from fermentation process.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1013-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis

Harvianto

Haider

Jiang

et al. 2018

Biotechnol Biofuels

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“…2,3-butanediol has been demonstrated as a promising, non-cytotoxic liquid fuel and bulk chemical for various applications including the use as food additive and antifreeze agent [ 4 ] or as precursor for the formation of methyl ethyl ketone [ 5 ]. The major drawback of natural 2,3-butanediol producers, for instance Klebsiella oxytoca [ 6 ], Klebsiella pneumoniae [ 7 ], Serratia marcescens [ 8 ], Enterobacter aerogenes [ 9 ] or Enterobacter cloacae [ 10 ], is that these organisms often require complex and expensive medium components (Table 1 ). Also the pathogenic nature of these strains limits the use as industrial production hosts [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

2018

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BackgroundEfficient microbial production of chemicals is often hindered by the cytotoxicity of the products or by the pathogenicity of the host strains. Hence 2,3-butanediol, an important drop-in chemical, is an interesting alternative target molecule for microbial synthesis since it is non-cytotoxic. Metabolic engineering of non-pathogenic and industrially relevant microorganisms, such as Escherichia coli, have already yielded in promising 2,3-butanediol titers showing the potential of microbial synthesis of 2,3-butanediol. However, current microbial 2,3-butanediol production processes often rely on yeast extract as expensive additive, rendering these processes infeasible for industrial production.ResultsThe aim of this study was to develop an efficient 2,3-butanediol production process with E. coli operating on the premise of using cost-effective medium without complex supplements, considering second generation feedstocks. Different gene donors and promoter fine-tuning allowed for construction of a potent E. coli strain for the production of 2,3-butanediol as important drop-in chemical. Pulsed fed-batch cultivations of E. coli W using microaerobic conditions showed high diol productivity of 4.5 g l−1 h−1. Optimizing oxygen supply and elimination of acetoin and by-product formation improved the 2,3-butanediol titer to 68 g l−1, 76% of the theoretical maximum yield, however, at the expense of productivity. Sugar beet molasses was tested as a potential substrate for industrial production of chemicals. Pulsed fed-batch cultivations produced 56 g l−1 2,3-butanediol, underlining the great potential of E. coli W as production organism for high value-added chemicals.ConclusionA potent 2,3-butanediol producing E. coli strain was generated by considering promoter fine-tuning to balance cell fitness and production capacity. For the first time, 2,3-butanediol production was achieved with promising titer, rate and yield and no acetoin formation from glucose in pulsed fed-batch cultivations using chemically defined medium without complex hydrolysates. Furthermore, versatility of E. coli W as production host was demonstrated by efficiently converting sucrose from sugar beet molasses into 2,3-butanediol.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1038-0) contains supplementary material, which is available to authorized users.

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“…In the case of Enterobacter aerogenes, glucose is a preferable carbon source for growth (carbon catabolite repression) [44]. Hence, in its presence the other carbohydrates present in enzymatic hydrolysates of LCMs should not be consumed as efficiently as glucose in dark fermentation.…”

Section: Contentmentioning

confidence: 99%

“…alkaline-pre-treatment step were also analyzed with scanning electron microscopy (SEM), in order to investigate and compare noticeable differences in the surface structure of LCMs. In the case of Enterobacter aerogenes, glucose is a preferable carbon source for growth (carbon catabolite repression) [44]. Hence, in its presence the other carbohydrates present in enzymatic hydrolysates of LCMs should not be consumed as efficiently as glucose in dark fermentation.…”

Section: Contentmentioning

confidence: 99%

“…It seems, however, that it is possible to increase the amount of remaining sugars and increase the efficiency of hydrogen production. Jung et al [44] constructed a genetically modified E. aerogenes strain in which the mechanism of carbon catabolite repression was abolished and the substrate preferences were modified in order to better use the mixture of sugars found in the cassava molasses for the production of 2,3-butanediol by fermentation. An analogous procedure could be used to obtain genetically modified strains of E. aerogenes that in the glucose presence in enzymatic hydrolysates of LCMs efficiently assimilate various monosaccharides, i.e., xylose, and use it for the production of hydrogen.…”

Section: Process Coursementioning

confidence: 99%

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Fermentative Conversion of Two-Step Pre-Treated Lignocellulosic Biomass to Hydrogen

et al. 2019

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Fermentative hydrogen production via dark fermentation with the application of lignocellulosic biomass requires a multistep pre-treatment procedure, due to the complexed structure of the raw material. Hence, the comparison of the hydrogen productivity potential of different lignocellulosic materials (LCMs) in relation to the lignocellulosic biomass composition is often considered as an interesting field of research. In this study, several types of biomass, representing woods, cereals and grass were processed by means of mechanical pre-treatment and alkaline and enzymatic hydrolysis. Hydrolysates were used in fermentative hydrogen production via dark fermentation process with Enterobacter aerogenes (model organism). The differences in the hydrogen productivity regarding different materials hydrolysates were analyzed using chemometric methods with respect to a wide dataset collected throughout this study. Hydrogen formation, as expected, was positively correlated with glucose concentration and total reducing sugars amount (YTRS) in enzymatic hydrolysates of LCMs, and negatively correlated with concentrations of enzymatic inhibitors i.e., HMF, furfural and total phenolic compounds in alkaline-hydrolysates LCMs, respectively. Interestingly, high hydrogen productivity was positively correlated with lignin content in raw LCMs and smaller mass loss of LCM after pre-treatment step. Besides results of chemometric analysis, the presented data analysis seems to confirm that the structure and chemical composition of lignin and hemicellulose present in the lignocellulosic material is more important to design the process of its bioconversion than the proportion between the cellulose, hemicellulose and lignin content in this material. For analyzed LCMs we found remarkable higher potential of hydrogen production via bioconversion process of woods i.e., beech (24.01 mL H2/g biomass), energetic poplar (23.41 mL H2/g biomass) or energetic willow (25.44 mL H2/g biomass) than for cereals i.e., triticale (17.82 mL H2/g biomass) and corn (14.37 mL H2/g biomass) or for meadow grass (7.22 mL H2/g biomass).

show abstract

Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production

Cited by 41 publications

References 40 publications

Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis

Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis

Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

Fermentative Conversion of Two-Step Pre-Treated Lignocellulosic Biomass to Hydrogen

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