Alleviation of DSS-induced colitis in mice by a new-isolated Lactobacillus acidophilus C4

Liu, Qianqian; Jian, Wenwen; Wang, Lu; Yang, Shu-Fang; Niu, Yutian; Xie, ShuaiJing; Chen, Kun; Zhang, Yi; Guo, Yanan; Tu, Zeng

doi:10.3389/fmicb.2023.1137701

Cited by 8 publications

(3 citation statements)

References 73 publications

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“…IBD is a representative intestinal disease with increasing prevalence worldwide [ 1 ]. Recent studies show that probiotics such as Lactobacillus species are a safe treatment for IBD; indeed, data suggest that probiotics improve colitis in the DSS-induced mouse model and IBD patients by regulating the intestinal barrier, immune responses, and gut microbiota [ 36 , 37 ]. Recently, we demonstrated that the probiotic strain HY7718 suppresses inflammatory responses and maintains TJs between intestinal epithelial cells [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota

Kim,

Jeon,

Kim

et al. 2024

IJMS

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Inflammatory bowel disease (IBD), a chronic condition that causes persistent inflammation in the digestive system, is closely associated with the intestinal microbiome. Here, we evaluated the effects of Lactiplantibacillus plantarum HY7718 (HY7718) on IBD symptoms in mice with dextran sulfate sodium (DSS)-induced colitis. Oral administration of HY7718 led to significant improvement in the disease activity index score and the histological index, as well as preventing weight loss, in model mice. HY7718 upregulated the expression of intestinal tight junction (TJ)-related genes and downregulated the expression of genes encoding pro-inflammatory cytokines and genes involved in the TLR/MyD88/NF-κB signaling pathway. Additionally, HY7718 reduced the blood levels of pro-inflammatory cytokines, as well as reversing DSS-induced changes to the composition of the intestinal microbiome. HY7718 also increased the percentage of beneficial bacteria (Lactiplantibacillus and Bifidobacterium), which correlated positively with the expression of intestinal TJ-related genes. Finally, HY7718 decreased the population of pathogens such as Escherichia, which correlated with IBD symptoms. The data suggest that HY7718 improves intestinal integrity in colitis model mice by regulating the expression of TJ proteins and inflammatory cytokines, as well as the composition of the intestinal microflora. Thus, L. plantarum HY7718 may be suitable as a functional supplement that improves IBD symptoms and gut health.

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Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota

Kim,

Jeon,

Kim

et al. 2024

IJMS

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“…Probiotic LAB have been demonstrated to exert positive effects on the anti-oxidative 10 , anti-inflammatory 34 , and anticancer activities 35 . The antioxidant capabilities of probiotics have been evaluated through IC 26 , CFS 10 and CFE or their metabolites in vitro 36 .…”

Section: Discussionmentioning

confidence: 99%

The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM

Gu,

Wang,

Wang

et al. 2024

Sci Rep

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Strain-specific probiotics can present antioxidant activity and reduce damage caused by oxidation. Streptococcus alactolyticus strain FGM (S. alactolyticus strain FGM) isolated from the chicken cecum shows potential probiotic properties which have been previously demonstrated. However, the antioxidant properties of S. alactolyticus strain FGM remain unknown. In this view, cell-free supernatant (CFS), intact cells (IC) and intracellular extracts (CFE) of strain FGM and 3 strains of Lactobacillus (LAB) were prepared, and their scavenging capacities against DPPH, hydroxyl radicals and linoleic acid peroxidation inhibitory were compared in this study. The effects of strain FGM cell-free supernatant (FCFS) on NO production, activity of SOD and GSH-Px in RAW264.7 cells and LPS-induced RAW264.7 cells were analyzed. The metabolites in the supernatant were quantitated by N300 Quantitative Metabolome. It was shown that the physicochemical characteristics of CFS to scavenge DPPH, hydroxyl radicals, and linoleic acid peroxidation inhibitory were significantly stronger than that of IC and CFE in the strain FGM (P < 0.05), respectively 87.12% ± 1.62, 45.03% ± 1.27, 15.63% ± 1.34. FCFS had a promotional effect on RAW264.7 cells, and significantly elevated SOD and GSH-Px activities in RAW264.7 cells. 25 μL FCFS significantly promoted the proliferation of RAW264.7 cells induced by LPS, increased the activities of SOD and GSH-PX, and decreased the release of NO. Furthermore, among the differential metabolites of FCFS quantified by N300, 12 metabolites were significantly up-regulated, including lactic acid, indole lactic acid, linoleic acid, pyruvic acid etc., many of which are known with antioxidant properties. In conclusion, FCFS had good antioxidant properties and activity, which can be attributed to metabolites produced from strain FGM fermentation. It was further confirmed that S. alactolyticus strain FGM and its postbiotic have potential probiotic properties and bright application prospects in livestock and poultry breeding.

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“…On the other hand, some beneficial bacteria can improve colitis symptoms. This is the case with the Lactobacillus / Lactococcus group, where several members can alleviate DSS-induced colitis symptoms [ 63 ]. Our observation that YM consumption reduced Eubacterium rectale / Clostridium coccoides and Enterobacteriaceae groups and increased the Lactobacillus / Lactococcus group could in part explain the beneficial properties of the medicinal plant since controlling dysbiosis and stimulating the presence of favorable bacteria could improve colitis symptoms [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Yerba Mate (Ilex paraguariensis) Reduces Colitis Severity by Promoting Anti-Inflammatory Macrophage Polarization

Olate-Briones,

Albornoz-Muñoz,

Rodríguez-Arriaza

et al. 2024

Nutrients

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Yerba Mate (YM) (Ilex paraguariensis) is a natural herbal supplement with a well-described anti-inflammatory capacity and beneficial effects in different inflammatory contexts such as insulin resistance or obesity. However, whether YM could improve other inflammatory conditions such as colitis or the immune cell population that can be modulated by this plant remains elusive. Here, by using 61 male and female C57BL/6/J wild-type (WT) mice and the dextran sodium sulfate (DSS)-induced acute colitis model, we evaluated the effect of YM on colitis symptoms and macrophage polarization. Our results showed that the oral administration of YM reduces colitis symptoms and improves animal survival. Increasing infiltration of anti-inflammatory M2 macrophage was observed in the colon of the mice treated with YM. Accordingly, YM promoted M2 macrophage differentiation in vivo. However, the direct administration of YM to bone marrow-derived macrophages did not increase anti-inflammatory polarization, suggesting that YM, through an indirect mechanism, is able to skew the M1/M2 ratio. Moreover, YM consumption reduced the Eubacterium rectale/Clostridium coccoides and Enterobacteriaceae groups and increased the Lactobacillus/Lactococcus group in the gut microbiota. In summary, we show that YM promotes an immunosuppressive environment by enhancing anti-inflammatory M2 macrophage differentiation, reducing colitis symptoms, and suggesting that YM consumption may be a good cost-effective treatment for ulcerative colitis.

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Alleviation of DSS-induced colitis in mice by a new-isolated Lactobacillus acidophilus C4

Cited by 8 publications

References 73 publications

Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota

Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota

The antioxidant activity and metabolomic analysis of the supernatant of Streptococcus alactolyticus strain FGM

Yerba Mate (Ilex paraguariensis) Reduces Colitis Severity by Promoting Anti-Inflammatory Macrophage Polarization

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