Alleviation of Ultraviolet B-Induced Photodamage by Coffea arabica Extract in Human Skin Fibroblasts and Hairless Mouse Skin

Wu, Po-Yuan; Huang, Chi‐Chang; Yin, Chang; Huang, Ya-Han; Lin, Ping; Liu, Yuhan; Wen, Kuo‐Ching; Lin, Chang-Shen; Hsu, Mei‐Chich; Chiang, Hsiu-Mei

doi:10.3390/ijms18040782

Cited by 47 publications

(47 citation statements)

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“…Th cells were thought to lose this regulatory function owing to a reduction in Tregs; this would result in overexpression of Th2 cells and increased IL-6 levels, thereby. Increased IL-6 induces the degradation of collagen in the skin [38, 39]. Therefore, in stressed arthritic mice, this mechanism may have contributed to the deterioration of dry skin.…”

Section: Discussionmentioning

confidence: 99%

Deterioration of dry skin in arthritis model mice via stress-induced changes in immune cells in the thymus and spleen

Goto

Hiramoto

Takada

et al. 2019

Preprint

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19Skin dryness is a characteristic of rheumatoid arthritis model mice. We previously reported 20 that the stress hormone glucocorticoid (i.e., corticosterone) is related to the induction of dry 21 skin in arthritic mice. However, the mechanism through which stress induces dry skin in 22 these mice is still unclear. Therefore, in this study, we examined the relationship between 23 stress and induction of dry skin in arthritic mice. Physical stress load in mice with 24 DBA/1JJmsSlc collagen-induced arthritis was treated with water immersion stress, and 25 transepidermal water loss and the expression of markers associated with allergic reactions 26 and inflammation was evaluated. Deterioration of skin dryness was observed in stressed 27 arthritic mice compared with that in unstressed arthritic mice. Moreover, plasma levels of 28 interleukin-6 and corticosterone were increased in stressed arthritic mice compared with 29 those in unstressed arthritic mice. We also observed decreased regulatory T cell numbers and 30 increased T helper type 2 cell numbers in the thymus of stressed arthritic mice compared with 31 those in unstressed arthritic mice. These results suggested that abnormalities in the immune 32 system were related to deterioration of dry skin in stressed arthritic mice. Thus, reduction of 33 stress may prevent deterioration of dry skin in mice with arthritis. 34 36 37 corticotropin-releasing hormone production, leading to increased secretion of 57 adrenocorticotropic hormone by the pituitary gland [12]. These changes caused increased 58 synthesis and secretion of GC from the adrenal glands. GC has anti-inflammatory effects, but 59 induces abnormalities in the structure and function of the skin by upregulating Toll-like 60 receptor 2 [13]. Furthermore, treatment with the GC receptor (GCR) inhibitor RU486 61ameliorates dry skin in arthritic mice [11]. Accordingly, stress is related to induction of dry 62 skin in arthritic mice. Additionally, stress is involved in the onset of RA itself [14]. However, 63 despite these studies, the mechanisms through which stress induces dry skin in arthritic mice 64 are still unclear. 65In this study, we evaluated the effects of water immersion stress on dry skin in 66 arthritic mice. 67 MATERIALS AND METHODS 68Animals 69 Specific pathogen-free DBA/1JJmsSlc mice (10 weeks old) were obtained from Japan SLC 70 (Hamamatsu, Shizuoka, Japan). We used these mice as a collagen-induced arthritic mouse 71 model. The method for inducing arthritis in these mice was described previously [11]. The 72 mice were housed under a 12:12-h light/dark cycle at a constant temperature of 23°C ± 2°C 73 and 55% ± 10% relative humidity. The mice had free access to water and laboratory chow 74 (CE-2; Oriental Yeast Co., Tokyo, Japan). All animals were treated in accordance with the 76 (approval number: 66). All experiments were performed under sodium pentobarbital 77 anesthesia. 78Experimental design 79 After allowing the mice to acclimate for 1 week, stress treatment was applied for 4 days, and 80 mi...

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Section: Discussionmentioning

confidence: 99%

Deterioration of dry skin in arthritis model mice via stress-induced changes in immune cells in the thymus and spleen

Goto

Hiramoto

Takada

et al. 2019

Preprint

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“…Also, chronic exposure of the skin to UV radiation stimulates mitogen‐activated protein kinase signaling pathway resulting in the induction of MMPs, such as MMP‐1, also known as interstitial collagenase, which is responsible for the degradation of dermal type 1 collagen that is the most abundant structural protein in the dermis (Jung, Lee, Lee, & Cho, ). The topical application of natural products with high antioxidant and anti‐inflammatory activity may ameliorate UV‐induced photodamage (Wu et al, ). In this study, we also found that topical administration of DLE, CLE, or DLE/CLE prevented UVB‐induced skin damage, epidermal hyperplasia, and collagen degradation in the skin of SHK‐1 mice.…”

Section: Discussionmentioning

confidence: 99%

“…S1 and , Tables S1 and ) interstitial collagenase, which is responsible for the degradation of dermal type 1 collagen that is the most abundant structural protein in the dermis (Jung, Lee, Lee, & Cho, 2016). The topical application of natural products with high antioxidant and anti-inflammatory activity may ameliorate UV-induced photodamage (Wu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Photoprotective properties of combined extracts fromDiospyros lotusleaves andCurcuma longarhizomes against chronic UVB‐induced photodamage

et al. 2018

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The synergistic effects of combined ethanolic extracts of Diospyros lotus leaves (DLE) and Curcuma longa rhizomes (CLE) were investigated for their protective effects on chronic Ultraviolet‐B (UVB)‐induced skin photodamage in SHK‐1 mice. The degree of protection was measured by using biochemical parameters such as malondialdehyde, reduced glutathione, superoxide dismutase, catalase, Akt activation, and nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2) activities, collagen degradation among others. DLE, CLE, and DLE/CLE demonstrated significant photodamage protection by upregulating endogenous antioxidant in mice skin through the activation of Akt and Nrf2 activities; prevented epidermal hyperplasia; mast cell infiltration into the skin and collagen degradation. The effects of the combined extract were greater than the effects of the single extracts. Also, the synergistic effects of DLE/CLE were comparable to that of standard drug, ascorbic acid. This study suggests the synergistic protective role of DLE/CLE in the treatment UVB‐induced photodamage. Practical application The use of synthetic antioxidant products to prevent oxidative‐related stress, especially from UV radiations is frequently accompanied with severe side effects. Therefore, the search for natural antioxidants is of utmost importance. Polyphenols are of great interest due to their antioxidant properties and small molecular weight that permits them to penetrate the skin. Diospyros lotus (rich in myricitrin) and Curcuma longa rhizomes (rich in curcumin) are both edible plants. This research investigates the synergetic effects of extracts of both plants against chronic UVB‐induced oxidative stress. Data obtained from this study shed light on using plant‐based products both alone and in synergy as potential functional foods and nutraceuticals for the prevention of UVB‐induced oxidative stress. The results of this research could serve as a base for further research on these extracts as a source of antioxidant and promotes the usage of these plants on a large scale as functional food materials.

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“…After the cells were treated for 24 h, the effect of G6 on cell viability was monitored by the MTT assay. MTT solution (5 mg/mL in PBS buffer) was added and incubated for 4 h. Then, the medium was removed and replaced with DMSO to dissolve the potassium salt and the absorbance was measured at 570 nm [70,71].…”

Section: Mtt Assaymentioning

confidence: 99%

Protective Effect of l-Hexaguluroic Acid Hexasodium Salt on UVA-Induced Photo-Aging in HaCaT Cells

Bai

Qin

et al. 2020

IJMS

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This study aimed to show the α-l-Hexaguluroic acid hexasodium salt (G6) protective effect against UVA-induced photoaging of human keratinocyte cells. We found that G6 localized to the mitochondria and improved mitochondrial functions. G6 increased respiratory chain complex activities, which led to increased cellular ATP content and NAD + /NADH ratio. Thus, G6 alleviated the oxidative stress state in UVA-irradiated cells. Moreover, G6 can regulate the SIRT1/pGC-1α pathway, which enhanced the cells' viability and mitochondria energy metabolism. Notably, the anti-photoaging potential of G6 was directly associated with the increased level of MMP and SIRT1, which was followed by the upregulation of pGC-1α, D-LOOP, and Mt-TFA, and with the transcriptional activation of NRF1/NRF2. Taking all of the results together, we conclude that G6 could protect HaCaT cells from UVA-induced photo-aging via the regulation of mitochondria energy metabolism and its downstream signaling pathways.Int. J. Mol. Sci. 2020, 21, 1201 2 of 16 breathing, a small fraction of electrons may "leak" from the electron transport chain (ETC), especially from complexes I and III reduce oxygen to form ROS [13]. Other mitochondrial proteins, such as alpha-glycerophosphate dehydrogenase, alpha-ketoglutarate, and pyruvate dehydrogenase, have been shown to play a role in ROS production [14]. The mitochondria are also the only organelle in animal cells that have their own mtDNA, which is located physically close to the mitochondrial respiratory chain (MRC). Moreover, those point mutations and deletions in mtDNA were reported to accumulate more and more in various tissues during aging [15], which might consequently lead to the declined MRC capacity in various tissues, such as the skeletal muscle and liver [16,17]. In addition, the fact that the mitochondrial ETC is the major ROS production site results in mitochondria being the primary target for oxidative damage. Related studies have shown that with age, mitochondria become larger, decrease in the number, accumulate vacuoles, sputum abnormalities, and experience mitochondrial rupture [18,19].More importantly, oxidative damage of protein and mtDNA, accompanied by mtDNA mutations, could result in a decrease in mitochondrial respiratory chain enzyme activities, mitochondrial membrane potential, and ATP production [19]. It has been demonstrated that creatine enhances DNA repair caused by UV-induced stress exposure in skin models [20]. Notably, in human clinical settings, formulations containing creatine, acetyl l-carnitine, and NADH can reduce the appearance of aging [21]. The researchers believe that the repair is due to the increased availability of ATP provided by creatine. Under the pressure of ultraviolet light, cells can use this additional ATP to synthesize the required repair enzymes [20,21].Meanwhile, ultraviolet irradiation can also induce the expression of the ROS production, leading to skin inflammation and downregulation of SIRT1 [22]. It has been reported that an enhancement in sirtuin genes and pGC...

show abstract

Alleviation of Ultraviolet B-Induced Photodamage by Coffea arabica Extract in Human Skin Fibroblasts and Hairless Mouse Skin

Cited by 47 publications

References 55 publications

Deterioration of dry skin in arthritis model mice via stress-induced changes in immune cells in the thymus and spleen

Deterioration of dry skin in arthritis model mice via stress-induced changes in immune cells in the thymus and spleen

Photoprotective properties of combined extracts fromDiospyros lotusleaves andCurcuma longarhizomes against chronic UVB‐induced photodamage

Protective Effect of l-Hexaguluroic Acid Hexasodium Salt on UVA-Induced Photo-Aging in HaCaT Cells

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