Allicin Reduces the Production of α-Toxin by Staphylococcus aureus

Leng, Bingfeng; Qiu, Jiazhang; Dai, Xianzhong; Dong, Jing; Wang, Jianfeng; Luo, Mingjing; Li, Hong-En; Niu, Xiaodi; Zhang, Yu; Ai, Yongxing; Deng, Xuming

doi:10.3390/molecules16097958

Cited by 41 publications

(30 citation statements)

References 33 publications

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“…Staphylococcus aureus strain 8325-4 was grown in TSB with or without apigenin to OD 600 nm of 2.5. Total bacterial RNA was extracted as described previously (Leng et al, 2011). Cells were harvested by centrifugation (5000 g for 5 min at 4°C) and resuspended into TES buffer (10 mM Tris-Cl, 1 mM EDTA, 0.5% SDS) containing 100 lg mL À1 of lysostaphin (Sigma-Aldrich) for 10 min, and then the samples were applied to a Qiagen RNeasy Maxi column (Qiagen, Hilden, Germany) to isolate total RNA following the manufacturer's instructions.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Apigenin alleviates the symptoms ofStaphylococcus aureuspneumonia by inhibiting the production of alpha-hemolysin

Dong

Qiu

Wang

et al. 2012

FEMS Microbiol Lett

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Staphylococcus aureus is a common human pathogenic bacteria that can cause serious infections, including lethal staphylococcal pneumonia. The development of antimicrobial resistance has limited treatment options for this pathogen; consequently, novel antibiotics and strategies are urgently desired to combat these infections. In recent years, virulence factors secreted by pathogenic microorganisms have been developed as targets for drug discovery. Alpha-hemolysin, a pore-forming cytotoxin that is secreted by most S. aureus strains, is essential for the pathogenesis of S. aureus pneumonia. In this study, we report that apigenin, a compound extracted from parsley that has no antimicrobial activity vs. S. aureus in vitro, can remarkably decrease the production of α-hemolysin at low concentrations. When added to the A549 cells and S. aureus co-culture system, apigenin protected A549 cells from α-hemolysin-mediated injury. Furthermore, in vivo tests indicated that apigenin alleviated injury of the lung tissue and decreased cytokine levels in the bronchoalveolar lavage fluid in the mouse model of S. aureus pneumonia.

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Section: Real-time Rt-pcrmentioning

confidence: 99%

Apigenin alleviates the symptoms ofStaphylococcus aureuspneumonia by inhibiting the production of alpha-hemolysin

Dong

Qiu

Wang

et al. 2012

FEMS Microbiol Lett

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“…Alpha-toxin is a pore-forming hemolytic toxin that brings about membrane damage to many types of mammalian cells. Alpha hemolysin toxin can be increased the productivity by cloning (Leng et al, 2011;Tavares et al, 2014;Gurnev and Netorovich, 2014;Kong et al, 2016). Alpha hemolysin toxin plays a role in inducing apoptosis in tumor cells, so it can be used as anticancer .…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of Alpha Hemolysin Toxin Gene of Staphylococcus aureus Against Human Cancer Tissue

Abdel-Fattah

Hafez

Zaki

et al. 2017

Int J Appl Sci Biotechnol

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Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. In our study, alpha hemolysin toxin (hia) gene was isolated from S. aureus isolate. The isolate of S. aureus was isolated from blood samples of patients at Microbiology laboratory of Chlidren Mansoura Unversity Hospital. The purified PCR of hia gene of S. aureus strain was subjected to sequencing and cloning. Recombinant alpha hemolysin toxin cloning was detected by PCR, and α-hemolysin protein was purified by Sphadex. The purified α-protein was used as anticancer against HepG-2 cells (human Hepatocellular carcinoma), HCT-116 cells (human colon carcinoma), MCF-7 cells (human breast cancer cell line) and A-549 cells (human Lung cancer cell line).

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“…,b, ,b), allicin (Leng et al . ), PNCB seems to have a similar influential effect on antimicrobial testing. In agreement with these considerations, these data suggest that the development of cell‐based therapy as a strategy to restore or protect the alveolar epithelium in injured lung tissue may be beneficial.…”

Section: Discussionmentioning

confidence: 74%

Subinhibitory concentrations of pinocembrin exert anti-Staphylococcus aureus activity by reducing α -toxin expression

et al. 2013

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Aims: Natural products have been used as potentially important sources of novel antibacterials in combating pathogenic Staphylococcus aureus isolates, a major problem around the world. In this study, we aimed to investigate the antibacterial effects of pinocembrin (PNCB) against Staph. aureus pneumonia in a murine model and its influence on the production of Staph. aureus a-haemolysin (Hla). Methods and Results:The in vitro activities of PNCB on a-haemolysin production were determined using haemolysis, Western blot and real-time RT-PCR assays. The viability and cytotoxicity assays were performed to evaluate the influence of PNCB on a-toxin-mediated injury of human alveolar epithelial cells. Moreover, through histopathologic analysis, we further determined the in vivo effects of PNCB on Staph. aureus pneumonia in a mouse model. In vitro, PNCB at low concentrations exhibited inhibitory activity against a-haemolysin production and attenuated a-haemolysinmediated cell injury. Furthermore, the in vivo findings demonstrated that PNCB protected mice from Staph. aureus pneumonia. Conclusions: We have provided new evidence of the effects of PNCB, which suggest that PNCB attenuated a-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia. Significance and Impact of the Study: The findings indicate that PNCB may be used as a basis for anti-Staphylococcus agent.

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Allicin Reduces the Production of α-Toxin by Staphylococcus aureus

Cited by 41 publications

References 33 publications

Apigenin alleviates the symptoms ofStaphylococcus aureuspneumonia by inhibiting the production of alpha-hemolysin

Apigenin alleviates the symptoms ofStaphylococcus aureuspneumonia by inhibiting the production of alpha-hemolysin

Cloning and Expression of Alpha Hemolysin Toxin Gene of Staphylococcus aureus Against Human Cancer Tissue

Subinhibitory concentrations of pinocembrin exert anti-Staphylococcus aureus activity by reducing α -toxin expression

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