Allium macrostemon whole extract ameliorates obesity-induced inflammation and endoplasmic reticulum stress in adipose tissue of high-fat diet-fed C57BL/6N mice

Kim, Choon Young; Kim, Juhae; Lee, Joo-Yeon

doi:10.29219/fnr.v67.9256

Cited by 5 publications

(7 citation statements)

References 70 publications

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“…It is believed that polyphenols in AM extract are responsible for its biological function. We previously reported the results of HPLC analysis showing that the AM contained several phenolic compounds including ellagic acid, protocatechuic acid, catechin, ferulic acid, chlorogenic, p-coumaric acid, and caffeic acid which had anti-obesity, antioxidant and anti-in ammatory activities (Kim et al 2023). Herein, we further analyzed AM by UHPLC-mass spectrometry and molecular networking analysis.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Kim

Youn

Seo

et al. 2023

Preprint

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Exposure of humans to high-fat (HF) diet and bisphenol A (BPA) has increased in recent times, and co-exposure to these causes harmful tissue toxicity. Here, the preventive effect of Allium macrostemon (AM) extract against dysfunction of adipose tissue and the liver under co-exposure to BPA and HF diet was examined. C57BL/6 mice were fed control (CON) diet, HF diet, HF diet with an oral injection of BPA (HF + BP), or HF diet with an oral co-injection of BPA and AM extract (HF + BP + AM). While HF feeding increased body weight gain compared to CON feeding, BP + HF suppressed body weight gain. BP + HF with lower body weight than HF had similar epididymal fat mass and inflammatory stress as HF, but significantly higher serum triglyceride and free fatty acid levels. In the liver, altered endoplasmic reticulum (ER) stress response and decreased autophagy were observed in HF + BP, as shown by increased t-IRE1α, p-JNK, and p62 levels and decreased beclin-1 protein levels, compared to those in HF. HF + BP + AM reduced HF and BPA-induced pro-inflammatory responses in adipose tissue, ER stress in liver, and total cholesterol level in serum. In addition, ecdysterone 25-O-β-D-glucopyranoside and 6-gingerol were firstly identified in AM extract by mass spectrometry and molecular networking analysis. In summary, the AM extract diminished liver and adipose tissue dysregulation, including hepatic ER stress and adipose tissue inflammation while a HF diet and BPA co-exposure induced harmful health outcomes in adipocytes and the liver. Therefore, AM has the potential to alleviate the harmful effects of a HF diet and BPA via anti-ER stress and anti-inflammatory activities.

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Section: Discussionmentioning

confidence: 99%

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Kim

Youn

Seo

et al. 2023

Preprint

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“…The yield was 8.53% of the initial dry weight of the Cibotium Rhizome. The final extract was stored at −20 • C until use [22].…”

Section: Sample Preparationmentioning

confidence: 99%

“…Absorbance was monitored at 280 nm for protocatechuic acid, (+)-catechin hydrate, p-coumaric acid, and ellagic acid, and at 320 nm for chlorogenic acid, caffeic acid, and ferulic acid. The results were quantified using a curve corresponding to standard materials and are presented in micrograms per gram [22]. The quantitative curve equation for each standard is shown in Supplementary Table S1.…”

Section: Compound Analysis Of Ecr By Hplcmentioning

confidence: 99%

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Protective Role of Ethanol Extract of Cibotium barometz (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes

Kim,

Lee,

Kim

2023

IJMS

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Sarcopenia is a progressive muscle disease characterized by the loss of skeletal muscle mass, strength, function, and physical performance. Since the disease code was assigned, attention has been focused on natural products that can protect against muscle atrophy. Cibotium barometz (Cibotium Rhizome) has been used as an herbal medicine for the treatment of bone or joint diseases in Asian countries. However, no studies have identified the mechanism of action of Cibotium Rhizome on muscle atrophy related to sarcopenia at the site of myotubes. The aim of this study was to investigate the improvement effect of the ethanol extract of Cibotium Rhizome (ECR) on dexamethasone-induced muscle atrophy in an in vitro cell model, i.e., the C2C12 myotubes. High-performance liquid chromatography was performed to examine the phytochemicals in ECR. Seven peaks in the ECR were identified, corresponding to the following compounds: protocatechuic acid, (+)-catechin hydrate, p-coumaric acid, ellagic acid, chlorogenic acid, caffeic acid, and ferulic acid. In atrophy-like conditions induced by 100 μM dexamethasone for 24 h in C2C12, ECR increased the expression of the myosin heavy chain, p-Akt, the p-mammalian target of rapamycin (mTOR), p-p70S6K, and repressed the expression of regulated in development and DNA damage responses 1 (REDD1), kruppel-like factor 15 (KLF 15), muscle atrophy F-box, and muscle-specific RING finger protein-1 in C2C12. In addition, ECR alleviated dexamethasone-induced muscle atrophy by repressing REDD1 and KLF15 transcription in C2C12 myotubes, indicating the need for further studies to provide a scientific basis for the development of useful therapeutic agents using ECR to alleviate the effects of skeletal muscle atrophy or sarcopenia.

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“…Allium macrostemon Bunge (AM), one of the plant species included in the Chinese Pharmacopoeia [23], is commonly grown in East Asia [24] and has shown a variety of beneficial effects, including lipid-lowering, antioxidant, and anti-obesity activities, in various study models, including in vitro, in vivo, and clinical systems [25][26][27][28][29]. Previously, we reported that AM whole extract (200 mg/kg BW daily) treatment ameliorates inflammation and endoplasmic reticulum stress in adipose tissue of HF diet-induced C57BL/6N mice [29]. Here, we hypothesized that the simultaneous exposure to HF and BPA from delivery containers and delivery foods in humans would be higher than before, as the delivery food culture increased rapidly as a result of COVID-19.…”

Section: Introductionmentioning

confidence: 99%

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

Kim,

Youn

et al. 2023

Foods

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The simultaneous exposure to a high-fat (HF) diet and to bisphenol A (BPA) from delivered foods and food-delivery containers is on the rise in humans, according to the increased frequency of food delivery during the COVID-19 pandemic. This co-exposure could cause harmful tissue toxicity in the human body. Here, the preventive effect of Allium macrostemon Bunge (AM) extract against dysfunction in adipose tissue and the liver under co-exposure to BPA and an HF diet was examined using mice. C57BL/6N mice were divided into four groups (n = 6 or 7/group) according to diet and treatment: control diet with vehicle (CON), HF diet with vehicle (HF), HF diet with an oral injection of BPA (HF + BP), and HF diet with an oral injection of BPA and AM extract (HF + BP + AM). HF feeding increased body weight gain compared to CON feeding, while BP + HF and BP + HF + AM feeding suppressed body weight gain compared with HF feeding. The BP + HF group had lower body weight than the HF group, but the two groups had similar epididymal fat mass. The HF + BP + AM group showed lower pro-inflammatory gene expression levels in adipose tissue and epididymal fat mass compared to the HF + BP group. Altered endoplasmic reticulum (ER) stress response in the liver was partly observed in the HF + BP group, as shown by increased total phosphorylated Jun N-terminal kinase protein levels compared to those in the HF group. In addition, ecdysterone 25-O-β-D-glucopyranoside and 6-gingerol were identified in AM extract by mass spectrometry and molecular networking analysis. In summary, the AM extract diminished adipose tissue inflammation and hepatic ER stress in an HF diet and BPA co-exposure condition. To utilize AM as a potential food component to alleviate the harmful effect of an HF diet and BPA exposure, further research investigating the specific impact of AM extract supplementation using additional experimental groups or various treatment doses is warranted.

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Allium macrostemon whole extract ameliorates obesity-induced inflammation and endoplasmic reticulum stress in adipose tissue of high-fat diet-fed C57BL/6N mice

Cited by 5 publications

References 70 publications

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Protective Role of Ethanol Extract of Cibotium barometz (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

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