Allo‐immune anti‐Ia^k sera of individual mice detect HLA—DR‐associated polymorphism on human B cells

Abstract

A murine allo‐immune A.TH anti‐A.TL (anti‐Iak) serum pool and sera from individual immunized mice were tested on a large panel of human DR typed B cells, obtained from peripheral blood (PBL—B cells), and human lymphoid tumour cells. The anti‐Iak serum pool appeared to be a useful reagent both in cytotoxicity and immunofluorescence for the examination of the presence of class‐2 HLA molecules on tumour cells. Only one discrepancy was observed when the pool was tested in parallel with a xeno‐anti‐human B‐cell serum on the cells of 56 lymphoid tumours. Nine sera from individual mice were tested on a panel of 93 PBL—B cells. Some of these sera reacted significantly more weakly with human B cells positive for the DR5 and/or DR7 antigens.