Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria

Madigan, Michael T.; Absher, Jill N.; Mayers, Joseph E.; Asao, Marie; Jung, Deborah O.; Bender, Kelly S.; Kempher, Megan L.; Hayward, Mackenzie K.; Sanguedolce, Sophia A.; Brown, Abigail C.; Takaichi, Shinichi; Kurokawa, Ken; Toyoda, Atsushi; Mori, Hiroshi; Tsukatani, Yusuke; Wang-Otomo, Zheng-Yu; Ward, David M.; Sattley, W. Matthew

doi:10.1007/s00203-021-02715-7

Cited by 10 publications

(11 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Tch. tepidum MC T mat formed on the surface of a warm spring (52 C) constantly bathed with sulfide, dissolved CaCO 3 and other minerals and exposed to full sunlight (Madigan et al, 2022). However, this "high-light" environment is not the only habitat Tch.…”

Section: Calcium and The Ecology Of Psbmentioning

confidence: 99%

“…Allochromatium tepidum is a mildly thermophilic species of PSB isolated from a sulfidic New Zealand hot spring microbial mat composed primarily of the green sulfur bacterium Chlorobaculum tepidum (Madigan et al, 2022; Wahlund et al, 1991). Alc.…”

Section: Calcium and The Lh1–rc Complex From Allochromatium Tepidummentioning

confidence: 99%

“…vinosum and Alc. tepidum show an average nucleotide identity of nearly 91% (Madigan et al, 2022) and that the Alc. vinosum LH1 α1‐polypeptide contained most of the Ca 2+ ‐binding motif seen in the α‐polypeptides of Alc.…”

Section: Calcium In the Lh1–rc Of Mesophilic Psbmentioning

confidence: 99%

“…tepidum and, more recently, Allochromatium (Alc.) tepidum, isolated from sulfidic thermal springs in Yellowstone and New Zealand, respectively (Madigan, 1984(Madigan, , 1986Madigan et al, 1989Madigan et al, , 2022Sattley et al, 2022). Thermophilic PSB have also been observed and in one case cultured from several other hot springs in North America and worldwide (Castenholz, 2015;Castenholz & Pierson, 1995;Saini et al, 2020).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Calcium and the ecology of photosynthesis in purple sulfur bacteria

Madigan,

Sattley,

Kimura

et al. 2024

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

The ecological success of purple sulfur bacteria (PSB) is linked to their ability to collect near‐infrared solar energy by membrane‐integrated, pigment–protein photocomplexes. These include a Core complex containing both light‐harvesting 1 (LH1) and reaction centre (RC) components (called the LH1–RC photocomplex) present in all PSB and a peripheral light‐harvesting complex present in most but not all PSB. In research to explain the unusual absorption properties of the thermophilic purple sulfur bacterium Thermochromatium tepidum, Ca2+ was discovered bound to LH1 polypeptides in its LH1–RC; further work showed that calcium controls both the thermostability and unusual spectrum of the Core complex. Since then, Ca2+ has been found in the LH1–RC photocomplexes of several other PSB, including mesophilic species, but not in the LH1–RC of purple non‐sulfur bacteria. Here we focus on four species of PSB—two thermophilic and two mesophilic—and describe how Ca2+ is integrated into and affects their photosynthetic machinery and why this previously overlooked divalent metal is a key nutrient for their ecological success.

show abstract

Section: Calcium and The Ecology Of Psbmentioning

confidence: 99%

Section: Calcium and The Lh1–rc Complex From Allochromatium Tepidummentioning

confidence: 99%

Section: Calcium In the Lh1–rc Of Mesophilic Psbmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Calcium and the ecology of photosynthesis in purple sulfur bacteria

Madigan,

Sattley,

Kimura

et al. 2024

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…tepidum from the Mammoth Hot Springs of Yellowstone National Park, USA [ 14 ], Allochromatium ( Alc .) tepidum from a sulfidic hot spring in New Zealand [ 15 , 16 ], Caldichromatium ( Ccm. ) japonicum from the Nakabusa hot springs in the Northern Alps of Japan [ 17 ], and Hlr.…”

Section: Introductionmentioning

confidence: 99%

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species

et al. 2022

Self Cite

View full text Add to dashboard Cite

Halorhodospira (Hlr.) species are the most halophilic and alkaliphilic of all purple bacteria. Hlr. halochloris exhibits the lowest LH1 Qy transition energy among phototrophic organisms and is the only known triply extremophilic anoxygenic phototroph, displaying a thermophilic, halophilic, and alkaliphilic phenotype. Recently, we reported that electrostatic charges are responsible for the unusual spectroscopic properties of the Hlr. halochloris LH1 complex. In the present work, we examined the effects of salt and pH on the spectroscopic properties and thermal stability of LH1-RCs from Hlr. halochloris compared with its mesophilic counterpart, Hlr. abdelmalekii. Experiments in which the photocomplexes were subjected to different levels of salt or variable pH revealed that the thermal stability of LH1-RCs from both species was largely retained in the presence of high salt concentrations and/or at alkaline pH but was markedly reduced by lowering the salt concentration and/or pH. Based on the amino acid sequences of LH1 polypeptides and their composition of acidic/basic residues and the Hofmeister series for cation/anion species, we discuss the importance of electrostatic charge in stabilizing the Hlr. halochloris LH1-RC complex to allow it to perform photosynthesis in its warm, hypersaline, and alkaline habitat.

show abstract