Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Lin, Chen; Lin, Guiting; Lue, Tom F.

doi:10.1089/scd.2012.0176

Cited by 187 publications

(160 citation statements)

References 89 publications

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“…There have been at least 27 different studies where human mesenchymal stromal cells have been placed into immunocompetent hosts of different species (31). Interestingly, in many of these cases, the cells survived several weeks and even engrafted into host tissue (22).…”

Section: Discussionmentioning

confidence: 99%

Human mesenchymal stromal cells decrease mortality after intestinal ischemia and reperfusion injury

Markel

Crafts

Jensen

et al. 2015

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

Human mesenchymal stromal cells decrease mortality after intestinal ischemia and reperfusion injury

Markel

Crafts

Jensen

et al. 2015

Journal of Surgical Research

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“…MSCs secrete several cytokines and growth factors that provide beneficial paracrine effects [19]. MSC therapy is considered a novel therapeutic approach for the treatment of a number of bladder disorders [20][21][22][23][24][25][26][27][28][29][30]. However, no studies to date have investigated MSC therapy to treat IC.…”

mentioning

confidence: 99%

Mesenchymal Stem Cell Therapy Alleviates Interstitial Cystitis by Activating Wnt Signaling Pathway

Song

Lim

et al. 2015

Stem Cells and Development

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Interstitial cystitis (IC) is a syndrome characterized by urinary urgency, frequency, pelvic pain, and nocturia in the absence of bacterial infection or identifiable pathology. IC is a devastating disease that certainly decreases quality of life. However, the causes of IC remain unknown and no effective treatments or cures have been developed. This study evaluated the therapeutic potency of using human umbilical cord-blood-derived mesenchymal stem cells (UCB-MSCs) to treat IC in a rat model and to investigate its responsible molecular mechanism. IC was induced in 10-week-old female Sprague-Dawley rats via the instillation of 0.1 M HCl or phosphate-buffered saline (PBS; sham). After 1 week, human UCB-MSC (IC + MSC) or PBS (IC) was directly injected into the submucosal layer of the bladder. A single injection of human UCB-MSCs significantly attenuated the irregular and decreased voiding interval in the IC group. Accordingly, denudation of the epithelium and increased inflammatory responses, mast cell infiltration, neurofilament production, and angiogenesis observed in the IC bladders were prevented in the IC + MSC group. The injected UCB-MSCs successfully engrafted to the stromal and epithelial tissues and activated Wnt signaling cascade. Interference with Wnt and epidermal growth factor receptor activity by small molecules abrogated the benefits of MSC therapy. This is the first report that provides an experimental evidence of the therapeutic effects and molecular mechanisms of MSC therapy to IC using an orthodox rat animal model. Our findings not only provide the basis for clinical trials of MSC therapy to IC but also advance our understanding of IC pathophysiology.

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“…7 The ADSCs surface marker HLA-DR is also negative, which implies low immunogenicity. 22 ADSCs can be used autologously without breaching any ethical standards. 23 In this study, passage 3 GFP-ADSCs were positive for CD90 and CD105, but negative for CD11b, CD31, CD34, CD83, and CD133.…”

Section: Discussionmentioning

confidence: 99%

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

Qin¹,

Li²,

Li³

et al. 2012

IJN

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Background: Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury. Methods: GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation. Results: GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging. Conclusion: Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.

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Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Cited by 187 publications

References 89 publications

Human mesenchymal stromal cells decrease mortality after intestinal ischemia and reperfusion injury

Human mesenchymal stromal cells decrease mortality after intestinal ischemia and reperfusion injury

Mesenchymal Stem Cell Therapy Alleviates Interstitial Cystitis by Activating Wnt Signaling Pathway

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

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