Allogeneic Fibroblasts Used to Grow Cultured Epidermal Autografts Persist in Vivo and Sensitize the Graft Recipient for Accelerated Second-Set Rejection

Abstract

Immunogenic fibroblasts used to grow CEAs survive in vivo and sensitize the graft recipient for accelerated second-set rejection. These persistent cells may initiate an inflammatory response that may result in late graft breakdown and limit the utility of CEAs grown with a foreign fibroblast feeder layer.

Human platelet lysate enhances the proliferative activity of cultured human fibroblast-like cells from different tissues

“…The use of cultured stromal cells from human adult tissues for cell-based therapies has a promising future for reconstituting damaged tissues. Nevertheless, the clinical use of FCS-cultured cells may lead to complications related to immune reactions (Spees et al 2004;Horwitz et al 2002;Hultman et al 1996). Spees et al have observed that the FCS proteins internalized by the cells were eliminated by further culturing with autologous serum and, furthermore, this process was optimized using conditions that promoted both metabolism and cell division (Spees et al 2004).…”

In vitro and in vivo characteristics of frozen/thawed neonatal pig split-skin strips: A novel biologically active dressing for areas of severe, acute or chronic skin loss

“…Unfortunately, the size of available donor sites is inversely proportional to the extent of burn wounds (2), and technical refinements, such as repeated harvesting of donor sites and meshed autograft expansion, only partly solve the problem, while bringing in the dangers of local desiccation and infection (3). On the other hand, cultured epithelial autografts (CEAs) have given unpredictable and inconsistent clinical results because of low 'take' rates (15-21%) due to non-optimal wound bed conditions (4), and second-set rejection responses evoked by the persistence of foreign (human or mouse) fibroblasts used as feeder layers (5). Allografts (or homografts) from living or dead donors are used by themselves or in association with autografts to cover extensive wounds (6).…”

“…Moreover, allografting entails the risk of transmitting infectious agents to the host (8). The use of cultured allo-epithelial grafts is hampered by the same restraints mentioned for allografts and CEAs (5). The exploit of using xenografts in burn care was pioneered by Reverdin in the 19th century (9).…”

“…Chemically-treated bovine skin (Dermodress ® ) has also been applied as a long-term skin substitute onto deep burn wounds (16). However, just as happens for allografts (5), permanent transplantation of animal skin is precluded by immunologic rejection (17). During the past 40 years, adult pig skin in various forms (i.e.…”

Preparation of Cultured Skin for Transplantation Using Insulin-like Growth Factor I in Conjunction with Insulin-like Growth Factor Binding Protein 5, Epidermal Growth Factor, and Vitronectin

“…Nevertheless, the time and cost associated with maintaining characterized stocks of serum and fibroblasts are considerable and perhaps even beyond the resources of many skin culture laboratories, especially in light of evolving regulatory requirements (12). Moreover, on a more practical level, there is evidence to suggest that the use of foreign materials in cultures contributes significantly to transplant rejection (13). The future of cultured skin transplants will therefore benefit greatly from the establishment of a technique based upon the use of a completely defined composition of reagents.…”