Allogeneic ovarian transplantation promotes the recovery of ovarian function in a rat model of cisplatin-induced premature ovarian failure

Abstract

Objective To investigate the effects of different sites of allogeneic ovarian transplantation on the recovery of ovarian function in the rat model of premature ovarian failure, and to evaluate its clinical value in the treatment of chemotherapy-induced ovarian dysfunction. Methods Thirty 2-month-old female SD rats of SPF grade were randomly divided into five groups, and both ovaries in the model group were treated with cisplatin (11 mg/kg) for modelling. After successful modelling, another 18 rats were taken as donor rats: group A was the control group, group B was the model group and group CDE was the experimental group. Ovary transplantation was performed using muscle, subcutaneous and renal peritoneum, respectively, and the rats were monitored for their general condition, estrous cycle and serum hormone levels after surgery. At the end of the observation period, the rats were executed and follicular development was observed. Results After 14 days of xenotransplantation, the general condition of the rats in the experimental group was better than that of the model group. Serum E2 and AMH levels were significantly higher than those in the model group (P = 0.000), and FSH levels were significantly lower than those in the control group (P = 0.000). Conclusion Cisplatin can be used to successfully establish the rat POF model; the three transplantation sites (muscle, subcutaneous and renal peritoneum) selected in this experiment can make the transplanted ovarian tissue survive and successfully restore the endocrine function of the body, and the effect of which showed no significant difference.