Allograft alone versus allograft with bone marrow concentrate for the healing of the instrumented posterolateral lumbar fusion

Hart, Radek; Komzák, Martin; Okál, František; Náhlík, David; Jajtner, Pavel; Puskeiler, M

doi:10.1016/j.spinee.2013.12.014

Cited by 44 publications

(45 citation statements)

References 27 publications

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“…Nucleated cells and particularly platelets, in the form of platelet lysate or platelet-rich plasma, have been shown to potentiate the therapeutic milieu of the BMC [27, 28, 53]. Furthermore, the extended array of cytokines and growth factors most likely work in concert with the various bone marrow cells to facilitate the overall regenerative process in vivo [21, 28, 54–56]. In this study, the concentration of nucleated cells and platelets increased significantly (2.6-fold and 11-fold, respectively) following processing with the Magellan, a fact that may prove beneficial for autologous cell therapy.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells

McDaniel

Antebi

Pilia

et al. 2017

Stem Cells International

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Background. One of the most plentiful sources for MSCs is the bone marrow; however, it is unknown whether MSC yield differs among different bone marrow sites. In this study, we quantified cellular yield and evaluated resident MSC population from five bone marrow sites in the porcine model. In addition, we assessed the feasibility of a commercially available platelet concentrator (Magellan® MAR01™ Arteriocyte Medical Systems, Hopkinton, MA) as a bedside stem cell concentration device. Methods. Analyses of bone marrow aspirate (BMA) and concentrated bone marrow aspirate (cBMA) included bone marrow volume, platelet and nucleated cell yield, colony-forming unit fibroblast (CFU-F) number, flow cytometry, and assessment of differentiation potential. Results. Following processing, the concentration of platelets and nucleated cells significantly increased but was not significantly different between sites. The iliac crest had significantly less bone marrow volume; however, it yielded significantly more CFUs compared to the other bone marrow sites. Culture-expanded cells from all tested sites expressed high levels of MSC surface markers and demonstrated adipogenic and osteogenic differentiation potential. Conclusions. All anatomical bone marrow sites contained MSCs, but the iliac crest was the most abundant source of MSCs. Additionally, the Magellan can function effectively as a bedside stem cell concentrator.

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Section: Discussionmentioning

confidence: 99%

Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells

McDaniel

Antebi

Pilia

et al. 2017

Stem Cells International

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“…In human medicine, interbody spinal fusion is promoted by several techniques. In addition to iliac crest autograft, metal and composite interbody cages, allograft bone dowels and bone grafts infused with recombinant bone morphogenetic proteins (BMPs) or bone marrow derived stem cells are readily available for human patients and show promising effects [ 18 , 34 ]. Moreover, in dogs the subchondral bone is relatively thicker than in humans whereas the canine end plates are thinner [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Pedicle screw-rod fixation: a feasible treatment for dogs with severe degenerative lumbosacral stenosis

et al. 2015

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BackgroundDegenerative lumbosacral stenosis is a common problem in large breed dogs. For severe degenerative lumbosacral stenosis, conservative treatment is often not effective and surgical intervention remains as the last treatment option. The objective of this retrospective study was to assess the middle to long term outcome of treatment of severe degenerative lumbosacral stenosis with pedicle screw-rod fixation with or without evidence of radiological discospondylitis.ResultsTwelve client-owned dogs with severe degenerative lumbosacral stenosis underwent pedicle screw-rod fixation of the lumbosacral junction. During long term follow-up, dogs were monitored by clinical evaluation, diagnostic imaging, force plate analysis, and by using questionnaires to owners.Clinical evaluation, force plate data, and responses to questionnaires completed by the owners showed resolution (n = 8) or improvement (n = 4) of clinical signs after pedicle screw-rod fixation in 12 dogs. There were no implant failures, however, no interbody vertebral bone fusion of the lumbosacral junction was observed in the follow-up period. Four dogs developed mild recurrent low back pain that could easily be controlled by pain medication and an altered exercise regime.ConclusionsPedicle screw-rod fixation offers a surgical treatment option for large breed dogs with severe degenerative lumbosacral stenosis with or without evidence of radiological discospondylitis in which no other treatment is available. Pedicle screw-rod fixation alone does not result in interbody vertebral bone fusion between L7 and S1.

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“…MSCs, isolated from bone marrow aspirate, have the ability to differentiate into the three classic mesenchymal lineages of bone, fat, and cartilage ; furthermore, their immunomodulatory characteristics suggest their potential in allogeneic transplantation . The study of MSC‐based therapies for bone regeneration has reached clinical trials for applications in, but not limited to, osteonecrosis , nonunion repair , and spinal fusion . MSCs, however, are a fairly rare population within bone marrow, comprising less than 0.01% of the nucleated population.…”

Section: Introductionmentioning

confidence: 99%

Platelet-Derived Growth Factor BB Enhances Osteogenesis of Adipose-Derived But Not Bone Marrow-Derived Mesenchymal Stromal/Stem Cells

et al. 2015

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Tissue engineering using mesenchymal stem cells holds great promise for regenerating critically sized bone defects. While the bone marrow-derived mesenchymal stem cell (MSC) is the most widely studied stromal/stem cell type for this application, its rarity within bone marrow and painful isolation procedure have motivated investigation of alternative cell sources. Adipose-derived stromal/stem cells (ASCs) are more abundant and more easily procured; furthermore, they also possess robust osteogenic potency. While these two cell types are widely considered very similar, there is a growing appreciation of possible innate differences in their biology and response to growth factors. In particular, reports indicate that their osteogenic response to platelet-derived growth factor BB (PDGF-BB) is markedly different: MSCs responded negatively or not at all to PDGF-BB while ASCs exhibited enhanced mineralization in response to physiological concentrations of PDGF-BB. In this study, we directly tested whether a fundamental difference existed between the osteogenic responses of MSCs and ASCs to PDGF-BB. MSCs and ASCs cultured under identical osteogenic conditions responded disparately to 20 ng/mL of PDGF-BB: MSCs exhibited no difference in mineralization while ASCs produced more calcium per cell. siRNA-mediated knockdown of PDGFRβ within ASCs abolished their ability to respond to PDGF-BB. Gene expression was also different; MSCs generally downregulated and ASCs generally upregulated osteogenic genes in response to PDGF-BB. ASCs transduced to produce PDGF-BB resulted in more regenerated bone within a critically sized murine calvarial defect compared to control ASCs, indicating PDGF-BB used specifically in conjunction with ASCs might enhance tissue engineering approaches for bone regeneration.

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Allograft alone versus allograft with bone marrow concentrate for the healing of the instrumented posterolateral lumbar fusion

Cited by 44 publications

References 27 publications

Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells

Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells

Pedicle screw-rod fixation: a feasible treatment for dogs with severe degenerative lumbosacral stenosis

Platelet-Derived Growth Factor BB Enhances Osteogenesis of Adipose-Derived But Not Bone Marrow-Derived Mesenchymal Stromal/Stem Cells

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