Allografting in the Pig

Calne, R. Y.

doi:10.1007/978-94-011-6600-3_13

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…Bom van Noordloos et al [18], Vaiman et al [19], Flye et al [20], and Bockhorn [21] have shown a dependence on the strain combina tion and SLA compatibility, which has been confirmed in the rat by Zimmerman et al [22] and Tsuchimoto et al [23], However, our own previous experience [7], and the present data accord with that of Caine et al [24], and shows that up to 60% unimmunosuppressed pigs survive despite highly stimu latory MLC responses between donor and recipient pre-operatively. This is also borne out by the fact that the occurrence of rejec tion in human liver allografts appears unre lated to the lymphocyte crossmatch or to MHC mismatch [25,26].…”

Section: Discussioncontrasting

confidence: 57%

“…This is also borne out by the fact that the occurrence of rejec tion in human liver allografts appears unre lated to the lymphocyte crossmatch or to MHC mismatch [25,26]. Fully SLA incom patible liver allografts can survive without immunological manipulation [19] while heart, kidney and skin grafts have been less successful [24], Survival is not dependent upon genetic relationship [18].…”

Section: Discussionmentioning

confidence: 99%

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Serial Mixed Lymphocyte Culture Responses in Pigs after Liver or Kidney Auto- or Allotransplantation

Bracher

Hickman²,

Terblanche³

1989

Eur Surg Res

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In order to investigate further the peculiarly limited response of the transplanted pig liver to rejection, this study investigated serial mixed lymphocyte culture (MLC) responses and suppression of phytohaemagglutinin (PHA)-stimulated lymphocytes by serum from transplanted animals. The responses of lymphocytes from liver allografted pigs were compared with those from sham-operated, liver-autografted or kidney auto- or allografted animals for up to 22 weeks postoperatively. Lymphocytes from operated animals were added to cultures of 2 ‘universal’ donors which were not anaesthetised at any time and which remained lymphocyte donors for the period of each operated pig’s survival. After liver allograft, a sustained stimulation of MLC responses was noted which contrasted with the minimal random stimulatory activity seen in the other groups. The response was not seen in lymphocytes from animals subjected to liver autograft. There was a surprising variability in the week-to-week MLC tests of individual animals for which no cause could be found. The serum from liver auto- and allografted pigs had suppressive action on the PHA stimulation of lymphocytes. There was no correlation between the length of survival and the intensity of the pre-operative MLC response. Whilst the biochemical data of liver allografted pigs showed evidence of ongoing hepatocyte damage, the histological changes were less obvious especially in the long-surviving group. This study highlights again the ability of unimmunosuppressed pigs to accept liver allograft and seems to associate this with sustained hepatocyte damage and stimulation of the MLC.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Serial Mixed Lymphocyte Culture Responses in Pigs after Liver or Kidney Auto- or Allotransplantation

Bracher

Hickman²,

Terblanche³

1989

Eur Surg Res

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Interactions between liver allografts and lymphocytotoxic alloantibodies in inbred rats

Houssin

Bellon

et al. 1986

Hepatology

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Several clinical and experimental findings suggest that liver allografts are less sensitive than other organ allografts to lymphocytotoxic antibodies. In this experimental study in hypersensitized inbred rat recipients, rejection of liver allografts was delayed compared to that of heart allografts. Furthermore, there was a marked decrease in the level of cytotoxic antibodies after liver allografting but not after heart allografting in these animals. The decrease in the level of antibodies also occurred after donor-specific extracorporeal liver hemoperfusion in hypersensitized recipients. Whether the decrease was caused by a massive absorption of antibodies on the liver or related to excretion of major histocompatibility complex antigens in a soluble form remains to be demonstrated. These results support the hypothesis that the liver has a privileged position in regard to rejection and are consistent with clinical observations made following ABO incompatible or crossmatch positive liver transplantations.Experimental studies in pigs (1) and rats (2, 3) have shown that the liver is less sensitive than other organ allografts to acute rejection. In preliminary experiments using rats hypersensitized by multiple skin allografts, we also have observed that liver allografts are less rapidly rejected than heart allografts (4). These experimental observations suggest t h a t the liver has a privileged position in regard to allograft rejection. This hypothesis of a privileged position agrees with two clinical observations: (i) the lesser severity and easier control of acute rejection of liver allografts compared t o that of other organ allografts (5) and (ii) the fact that, at variance with what occurs with kidney or heart allografts, hyperacute rejection of liver transplants is not usually observed in cases of ABO incompatibility or positive T-cell cross-match (6).In Experimental Protocol. Allogeneic hypersensitization was induced using repeated skin allografts according to theprotocol published by Guttmann (7): BN rats were grafted with a LEW skin graft on three successive occasions at 10-day intervals. Subsequent grafts in these animals were called donorspecific when the donor was of the same strain as that previously used to take skin allografts. ANIMALS AND METHODSTen days after the thud skin allograft, these hypersensitized BN rats were grafted, either with a LEW heart (Group 1) or a LEW liver allograft (Group 2). In the control groups, unsensitized BN rats were grafted with a LEW heart (Group 3) or a LEW liver allograft (Group 4). In a second series of experiments, LEW heart allografts were grafted in unsensitized BN rats after transfer of serum from hypersensitized rats just allografted with a LEW heart (Group 5) or a LEW liver allograft (Group 6). In Group 7, a donor-specific (LEW) extracorporeal liver hemoperfusion was performed in hypersensitized BN rats 10 days after the third LEW skin graft. One day later, a donor-specific (LEW) heart allograft was performed in these rats. The same protocol was applied in Group...

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Induction of Unresponsiveness to Organ Transplants in Congenic Strains of Rats and other Mammals

Thoenes

1975

Advances in Experimental Medicine and Biology

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Allografting in the Pig

Cited by 10 publications

References 19 publications

Serial Mixed Lymphocyte Culture Responses in Pigs after Liver or Kidney Auto- or Allotransplantation

Serial Mixed Lymphocyte Culture Responses in Pigs after Liver or Kidney Auto- or Allotransplantation

Interactions between liver allografts and lymphocytotoxic alloantibodies in inbred rats

Induction of Unresponsiveness to Organ Transplants in Congenic Strains of Rats and other Mammals

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