Allosteric control of 6-phosphofructo-1-kinase from rat lung

Abuelgassim, Abuelgassim O.; Salem, Ahmed M.; Khoja, Samir M.

doi:10.1016/0305-0491(92)90168-q

Cited by 2 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…on surfactant synthesis, which usually requires a high rate of glycolysis [9]. In recent studies, it was indeed illustrated that the number of alveoli of the lungs of the rat pups exposed to nicotine via the placenta and mother's milk was significantly lower than that of the control rat pups [17].…”

Section: Discussionmentioning

confidence: 98%

“…As a rate-limiting enzyme of glycolysis, adult rat lung PFK is highly controlled by its allosteric effectors, especially fructose-2,6-biphosphate, ADP, AMP, and in particular c-AMP; all were shown to be effective activators of PFK [9]. On the other hand, ATP is an allosteric inhibitor of PFK in adult rat lung [12,19].…”

Section: Discussionmentioning

confidence: 99%

“…is possibly also required for surfactant synthesis by the alveolar type II pneumocytes [9]. It is therefore conceivable that interference with carbohydrate metabolism may interfere with lung growth and development, as well as with the maintenance of the integrity of the type I pneumocytes and possibly with surfactant synthesis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Maternal Nicotine Exposure During Pregnancy and Lactation: I. Effect on Glycolysis in the Lungs of the Offspring

Kordom

Maritz

Kock

2003

Experimental Lung Research

View full text Add to dashboard Cite

We investigated the effect of maternal nicotine exposure during pregnancy and lactation on carbohydrate metabolism in the neonatal lung. Female rats received nicotine (1 mg/kg body weight/day) subcutaneously from day 7 after mating. Control animals received saline. The suckling rats were killed 24 hours after the last dose of nicotine was administered to the mother on postnatal day 14. The lung tissue of 9 rat pups from 3 litters was surgically removed and the in vitro utilization of exogenous glucose (micromol/g wet lung tissue/h) was determined. Lactate production (micromol/g wet lung tissue/h) was also determined to assess glycolytic activity. Maternal nicotine exposure during pregnancy and lactation stimulated glucose turnover by 21.6% (P<.01), but suppressed glycolysis by 24.6% (P<.001) and glycogenolysis by 37.9% (P<.001). Maternal nicotine exposure during gestation and lactation had no effect on the activity of hexokinase (U/g wet lung tissue), but resulted in a lower phosphofructokinase activity (U/g wet lung tissue) in the lungs of the offspring. From the data, it appeared that the inhibition of the flux of glucose through the glycolytic pathway can be attributed to an inhibition of phosphofructokinase.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Maternal Nicotine Exposure During Pregnancy and Lactation: I. Effect on Glycolysis in the Lungs of the Offspring

Kordom

Maritz

Kock

2003

Experimental Lung Research

View full text Add to dashboard Cite

show abstract

Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes

Nicolau¹,

Souza

Nogueira³

2006

Braz. oral res.

View full text Add to dashboard Cite

Although the influence of diabetes on salivary glands is well studied, it still presents conflicting results. In this work, the regulation of the phosphofructokinase-1 enzyme (PFK-1) was studied utilizing the salivary glands of rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/Kg of body weight) in rats (180-200 g). The animals were killed 30 days after the induction of diabetes and the submandibular and parotid salivary glands were used. Hyperglycemia was evaluated by blood sugar determination. The distribution of PFK-1 between the soluble and cytoskeleton fractions, the phosphate content of PFK-1, the content of fructose-2,6-bisphosphate and the activity of the PFK-2 enzyme were determined. The calculated relative glandular weight showed a higher value for the parotid gland in comparison with the control, but not for the submandibular gland. The activity of PFK-1 expressed per gland showed no variation between diabetic and control animals. However, considering the specific activity, the soluble enzyme presented a value 50% higher than that of the control and the cytoskeleton bound form increased by 84% compared to the control. For the parotid gland, no difference in the specific activity between diabetic and control animals was observed. On the other hand, the activity per gland of the soluble enzyme increased in the diabetic animals. The phosphate content of PFK-1 increased in the submandibular and parotid glands of diabetic rats. Both the content of fructose-2,6-bisphosphate and the active form of PFK-2 were reduced in the diabetic glands. In conclusion, the increase in the activity of PFK-1 observed in the salivary glands of rats with streptozotocin-induced diabetes does not seem to be due to its modulator fructose-2,6-bisphosphate.

show abstract

Allosteric control of 6-phosphofructo-1-kinase from rat lung

Cited by 2 publications

References 22 publications

Maternal Nicotine Exposure During Pregnancy and Lactation: I. Effect on Glycolysis in the Lungs of the Offspring

Maternal Nicotine Exposure During Pregnancy and Lactation: I. Effect on Glycolysis in the Lungs of the Offspring

Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes

Contact Info

Product

Resources

About