Allotetraploid hybrids produced by protoplast fusion for seedless triploid Citrus breeding

Wu, Jin-Hu; Ferguson, AR; Mooney, P. A.

doi:10.1007/s10681-005-7009-7

Cited by 26 publications

(19 citation statements)

References 14 publications

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“…Breeding triploid seedless Citrus cultivars producing highly sterile pollen and embryo sac has also been developed through hybridization of tetraploid and diploid cultivars Cameron, 1980, 1985). Non-conventional approaches for seedless citrus breeding include triploid embryo rescue (Aleza et al, 2010), protoplast fusion for producing tetraploid plants as breeding materials (Wu et al, 2005), cybrid with satsuma mandarin sterile cytoplasm (Cai et al, 2007;Guo et al, 2004) and intergeneric or wide crosses/fusions (Guo et al, 2002;Grosser et al, 1989). Induction of male sterility in Citrus by the chimeric ribonuclease gene has been applied in seeded 'Ponkan' (Li et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

Dewi

Wakana

Tanimoto

et al. 2013

J. Japan. Soc. Hort. Sci.

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In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R 1 and two complementary genes R 2 and R 3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R 3 is located downstream from R 2 , and gene R 1 is epistatic to R 2 and R 3 genes. Genotypes (S)r 1 r 1 r 2 r 2 --are responsible for undeveloped anthers while genotypes (S)r 1 r 1 R 2 -r 3 r 3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.

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Section: Introductionmentioning

confidence: 99%

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

Dewi

Wakana

Tanimoto

et al. 2013

J. Japan. Soc. Hort. Sci.

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“…Somatic hybridization via protoplast fusion enables recombination of nuclear and cytoplasmic genetic information of both parents, as provides an alternative strategy to bypass several difficulties, such as wide incompatibility, nucellar polyembryony and pollen/ovule sterility encountered in citrus conventional breeding. Now it has been a successful and promising biotechnique for citrus rootstock improvement as well as scion breeding (Grosser et al 2000;Grosser and Gmitter 2005;Guo et al 2004aGuo et al , b, 2008Wu et al 2005;Cai et al 2007;An et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

Cai

Duan

et al. 2009

Acta Physiol Plant

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Somatic hybridization by protoplast fusion from cell suspension cultures and leaf parent has been a well-established technique holding great potential for citrus variety improvement. In this study, somatic hybrid plants were regenerated from the following two fusion combinations: 'Murcott' tangor (Citrus reticulata Blanco 9 C. sinensis (L.) Osbeck) ? Hirado Buntan Pink pummelo (HBP) (C. grandis (L.) Osbeck) and 'Bingtang' orange (C. sinensis (L.) Osbeck) ? Calamondin (C. microcarpa Bunge). Somatic hybrids were selected at an early stage based on their higher capacity for embryogenesis comparing to non-hybrid cells. Flow cytometry analysis showed that all plants from pre-selected lines of the two combinations were tetraploid. SSR analysis confirmed their hybrid nature, with nuclear DNA from both fusion parents, and absence of parental specific bands was also detected. Cytoplasmic compositions of the recovered plants were further revealed by CAPS and cpSSR analysis. The allotetraploid somatic hybrids from the 'Murcott' tangor ? HBP combination will be applied to develop triploid seedless cultivars by interploid crossing with diploid seedy citrus cultivars, and those from 'Bingtang' orange ? Calamondin could be valuable for Asiatic citrus canker-tolerant and ornamental citrus breeding.

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“…In citrus breeding, new materials created by somatic hybridization have shown a bright future in new rootstock improvement, triploid seedless cultivar breeding, and creation of diploid cytoplasmic male sterile cybrids Guo et al 2004;Grosser and Gmitter 2005). To date, numerous intergeneric and interspecific citrus somatic hybrids have been recovered via somatic fusion Grosser and Gmitter 2005;Takami et al 2005;Wu et al 2005), including regenerated diploid cybrid plants from over 40 symmetrical fusion combinations Guo et al 2004).…”

Section: Introductionmentioning

confidence: 99%

GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage

Cai

Liu

Guo

2006

Plant Cell Tiss Organ Cult

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Somatic hybridization was performed via electrofusion between embryogenic suspension-derived protoplasts of transgenic green fluorescent protein (GFP) Satsuma mandarin (Citrus unshiu Marc. cv. Guoqing No. 1) (G1) callus and mesophyll protoplasts of calamondin (Citrus microcarpa Bunge), and three embryoids expressing GFP under UV light were obtained after 60 days of culture. The three embryoids were considered not as diploid cybrids but true allotetraploid somatic hybrids, as it was based on:(1) citrus heterokaryons are generally more vigorous and have higher capacity for embryogenesis as compared with unfused and homo-fused embryogenic callus protoplasts; (2) the callus line of G1 Satsuma mandarin has lost the embryogenesis capacity; and (3) citrus diploid cybrids produced by symmetric fusion always possess nuclear genome of mesophyll parent, and calamondin without GFP gene was used as leaf parent in this study. Subsequent flow cytometry, simple sequence repeat and cleaved amplified polymorphic sequence analysis of one regenerated callus mass and three resulting plants validated this supposition, i.e., the callus was derived from transgenic G1 callus protoplasts, and the three plants were true allotetraploid somatic hybrids possessing nuclear genomic DNA of both parents and cytoplasmic DNA from callus parent. The potential of transgenic GFP citrus callus as suspension parent in citrus somatic fusion to study the mechanism of cybrid formation, create new citrus cybrids, and transfer organelle-encoded agronomic traits was also discussed.

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Allotetraploid hybrids produced by protoplast fusion for seedless triploid Citrus breeding

Cited by 26 publications

References 14 publications

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage

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