Allyl isothiocyanate may reverse the expression of MRP1 in COPD rats via the Notch1 signaling pathway

Wu, Jie; Wang, Xueqi; Yao, Zhaomin; Wu, Qingqing; Fang, Wei; Li, Zegeng; Wang, Dianlei

doi:10.1007/s12272-019-01183-4

Cited by 11 publications

(5 citation statements)

References 34 publications

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“…Previous reports have suggested a potential role for MRP1 in smoking-related lung function loss and development of COPD. Reduced MRP1 protein levels were observed in lung tissue of COPD patients ( van der Deen et al, 2006 ) and in an experimental rat model of COPD ( Wu et al, 2019 ) using immunohistochemistry analysis. Moreover, the pulmonary clearance of inhaled [ 99 m Tc] methoxyisobutyl isonitrile ([ 99 m Tc] sestamibi), a radiolabeled MRP1 substrate, has been recorded to decrease in smokers and the decrement was attributed to modulation in MRP1 activity and expression profile ( Mohan et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been hypothesized that MRP1 protects lung cells against toxic insults of xenobiotics and from damage induced by oxidative stress by maintaining intracellular glutathione-glutathione disulfide homeostasis ( Cole and Deeley, 2006 ; Cole, 2014b ; Nickel et al, 2016 ). Inhibition of MRP1 was observed to worsen cigarette smoke extract (CSE)-induced cytotoxicity in vitro ( van der Deen et al, 2007 ) and pre-clinical and clinical data suggest that changes in abundance ( van der Deen et al, 2006 ; Wu et al, 2019 ) or function ( Budulac et al, 2010 ) of the transporter are associated with occurrence and severity of COPD. Moreover, recent in vivo data from our group showed that pulmonary distribution and clearance of the MRP1 substrate S -(6-(7-[ 11 C] methylpurinyl)) glutathione ([ 11 C]MPG), measured with positron emission tomography (PET), was significantly dependent on MRP1 abundance ( Mairinger et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Tobacco Smoke and Inhaled Drugs Alter Expression and Activity of Multidrug Resistance-Associated Protein-1 (MRP1) in Human Distal Lung Epithelial Cells in vitro

Selo

Delmas

Springer

et al. 2020

Front. Bioeng. Biotechnol.

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Multidrug resistance-associated protein-1 (MRP1/ABCC1) is highly expressed in human lung tissues. Recent studies suggest that it significantly affects the pulmonary disposition of its substrates, both after pulmonary and systemic administration. To better understand the molecular mechanisms involved, we studied the expression, subcellular localization and activity of MRP1 in freshly isolated human alveolar epithelial type 2 (AT2) and type 1-like (AT1-like) cells in primary culture, and in the NCI-H441 cell line. Moreover, the effect of cigarette smoke extract (CSE) and a series of inhaled drugs on MRP1 abundance and activity was investigated in vitro. MRP1 expression levels were measured by q-PCR and immunoblot in AT2 and AT1-like cells from different donors and in several passages of the NCI-H441 cell line. The subcellular localization of the transporter was studied by confocal laser scanning microscopy and cell surface protein biotinylation. MRP1 activity was assessed by bidirectional transport and efflux experiments using the MRP1 substrate, 5(6)-carboxyfluorescein [CF; formed intracellularly from 5(6)-carboxyfluorescein-diacetate (CFDA)] in AT1-like and NCI-H441 cell monolayers. Furthermore, the effect of CSE as well as several bronchodilators and inhaled corticosteroids on MRP1 abundance and CF efflux was investigated. MRP1 protein abundance increased upon differentiation from AT2 to AT1-like phenotype, however, ABCC1 gene levels remained unchanged. MRP1 abundance in NCI-H441 cells were comparable to those found in AT1-like cells. The transporter was detected primarily in basolateral membranes of both cell types which was consistent with net basolateral efflux of CF. Likewise, bidirectional transport studies showed net apicalto-basolateral transport of CF which was sensitive to the MRP1 inhibitor MK-571. Budesonide, beclomethasone dipropionate, salbutamol sulfate, and CSE decreased

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tobacco Smoke and Inhaled Drugs Alter Expression and Activity of Multidrug Resistance-Associated Protein-1 (MRP1) in Human Distal Lung Epithelial Cells in vitro

Selo

Delmas

Springer

et al. 2020

Front. Bioeng. Biotechnol.

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“…They used Notch1 signaling pathway inhibitor DAPT to treat human bronchial epithelial cells to verify the effect of Notch1 on AITC-induced MRP1 expression. Their research lacks necessary experimental data [ 3 ]. Shi et al believe that MicroRNA (miRNA) has become a new type of regulator in various pathological processes including ischemic stroke.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Diagnostic Medical System Based on Notch1 Signaling Pathway to Inhibit the Growth of Small-Cell Lung Carcinoma

Zhang

2022

Journal of Healthcare Engineering

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With the gradual application of big data and other technologies to the medical field, more and more people tend to get online medical services. This article mainly studies the comprehensive diagnostic medical system based on Notch1 signaling pathway to inhibit the growth of small-cell lung carcinoma. In the experiment, we used the rapid thawing method to recover the cells and took the logarithmic growth phase cells for cell passage. We calculated the cell concentration and diluted the cells according to the experimental requirements. According to the standard curve, the corresponding sample protein concentration was calculated; at the same time, the Trizol method was used to extract the total RNA, the NanoDrop8000 spectrophotometer was used to determine the RNA concentration, and the RNA quality was detected by agarose gel electrophoresis. We used immunohistochemical staining to complete the staining of lung cancer cells. Finally, black box testing was used to test the functional modules of the system. Experimental data show that the accuracy rate of data obtained by the system reaches 98%, which greatly facilitates doctors and patients. The results show that the system has good ease of use and reliability and improves the diagnosis and treatment of hospital patients.

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“…The trachea was cannulated and then connected to an animal lung function instrument (Bellambo Technology, Beijing, China). The parameters were recorded by software automatically including forced vital capacity (FVC), forced expiratory flows at 0.3 s /FVC (FEV 0.3/FVC%), peak expiratory flow (PEF), resistance of lung (RL), and respiratory dynamic compliance (Cdyn) as described earlier (Wang et al., 2017; Wu et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

Protective effects of Isodon Suzhouensis extract and glaucocalyxin A on chronic obstructive pulmonary disease through SOCS3–JAKs/STATs pathway

Zhai

Wang

Zheng³

et al. 2022

Food Frontiers

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Chronic obstructive pulmonary disease (COPD) is an irreversible disease with the leading causes of morbidity, mortality, and several physical limitations. Isodon Suzhouensis is a unique Chinese medicinal plant used for both medicine and food. It possesses strong anti‐inflammatory effects that may treat COPD. Considering the fact that the effects of Isodon Suzhouensis extract (WZZE) and its active ingredient glaucocalyxin A (GLA) on COPD have never been reported before, this study aimed to investigate the effects of WZZE and GLA against COPD and its underlying mechanistic pathway in COPD model rats or in CSE‐induced NR8383 cells. The variation in lung physiology was measured through spirometry while histopathological changes were studied via H&E staining of the COPD model rats lung samples. The levels of cytokine and other immunomodulatory proteins were determined by ELISA. Apoptosis of lung tissue cells was detected by TUNEL staining. Western blot and immunohistochemistry were used to detect the expression of related pathways and other proteins. The results show that WZZE improved the relevant lung function indicators, while restoring the activity of immunoglobulins (Ig A, IgG) and inhibiting the levels of TNF‐α and IL‐1β in serum and bronchoalveolar lavage fluid, thereby improving the body's immunity and inhibiting inflammation of the airways and lungs, slowing the progression of COPD. The results of H&E staining indicated that WZZE is capable of improving the pathological injury associated with COPD. In addition, the administration of WZZE reduced the expression of MUC5AC protein induced and the apoptosis rate of lung tissue cells in COPD model rats. The results also showed that WZZE significantly increased the expression of SOCS3 and inhibited the expression of JAKs/STATs pathway‐related proteins in COPD rats. In addition, GLA can also upregulate the expression of SOCS3 protein, inhibit JAKs/STATs pathway, inhibit the expression level of MUC5AC, upregulate the expression level of immunoglobulin, and downregulate the expression level of proinflammatory factors in NR8383 cells induced by CSE. Present study findings suggest that WZZE and GLA may ameliorate the progression of COPD via the SOCS3–JAKs/STATs pathway in COPD rats or in CSE‐induced NR8383.

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Allyl isothiocyanate may reverse the expression of MRP1 in COPD rats via the Notch1 signaling pathway

Cited by 11 publications

References 34 publications

Tobacco Smoke and Inhaled Drugs Alter Expression and Activity of Multidrug Resistance-Associated Protein-1 (MRP1) in Human Distal Lung Epithelial Cells in vitro

Tobacco Smoke and Inhaled Drugs Alter Expression and Activity of Multidrug Resistance-Associated Protein-1 (MRP1) in Human Distal Lung Epithelial Cells in vitro

Comprehensive Diagnostic Medical System Based on Notch1 Signaling Pathway to Inhibit the Growth of Small-Cell Lung Carcinoma

Protective effects of Isodon Suzhouensis extract and glaucocalyxin A on chronic obstructive pulmonary disease through SOCS3–JAKs/STATs pathway

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