Allyl isothiocyanate-rich mustard seed powder inhibits bladder cancer growth and muscle invasion

Bhattacharya, Arup; Li, Yun; Wade, Kristina L.; Paonessa, Joseph D.; Fahey, Jed W.; Zhang, Yuesheng

doi:10.1093/carcin/bgq202

Cited by 85 publications

(67 citation statements)

References 17 publications

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“…At present, the drugs used for the treatment of cervical cancer are often highly toxic and induce severe side effects, which may cause damage to patient tissues during the treatment of cancer and may reduce the chance of survival (2,3). Therefore, the identification of a safe, effective and low toxicity anticancer drug has attracted interest from researchers, and the development of these drugs from natural sources has become an important strategy in the development of antitumor drugs.…”

Section: Discussionmentioning

confidence: 99%

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Effect of allyl isothiocyanate on the viability and apoptosis of the human cervical cancer HeLa cell line inï¿½vitro

Qin

Xue

2018

Oncol Lett

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Abstract. The present study aimed to investigate the effect of allyl isothiocyanate (AITC) on the viability and apoptosis of the human cervical cancer HeLa cell line in vitro, and to explore the potential underlying mechanisms of this. HeLa cells were treated with varying concentrations of AITC for different durations. The cell viability was then measured using a Cell Counting kit-8 assay and the apoptosis rate of the cells was detected using flow cytometry. Additionally, the B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) mRNA expression levels were determined by reverse transcription-quantitative polymerase chain reaction, while the Bax and Bcl-2 protein expression levels in cells were detected by western blot analysis. AITC was revealed to inhibit the viability of HeLa cells. AITC was revealed to induce the apoptosis of HeLa cells, as the apoptosis rate increased gradually with an increase in the dose. As the concentration of AITC increased, the Bax mRNA expression level increased, whilst the Bcl-2 mRNA expression level decreased. Furthermore, the Bax protein expression intensity increased whilst Bcl-2 protein expression intensity decreased, thereby resulting in a decrease in the ratio of Bcl-2/Bax proteins. AITC may inhibit cell viability by inducing the apoptosis of HeLa cells and this may be accounted for by the imbalance in the Bcl-2/Bax expression ratio.

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Section: Discussionmentioning

confidence: 99%

“…Allyl isothiocyanate (AITC) is widely distributed in cruciferous plants and their by-products, including mustard and horseradish (2,3). A number of studies have considered AITC to be associated with tumors.…”

Section: Introductionmentioning

confidence: 99%

Effect of allyl isothiocyanate on the viability and apoptosis of the human cervical cancer HeLa cell line inï¿½vitro

Qin

Xue

2018

Oncol Lett

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“…Moreover, an AITC-rich mustard seed powder also strongly inhibited bladder cancer development and muscle invasion in vivo (3). Thus, AITC is highly promising for bladder cancer prevention and/or treatment.…”

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confidence: 95%

Allyl Isothiocyanate Arrests Cancer Cells in Mitosis, and Mitotic Arrest in Turn Leads to Apoptosis via Bcl-2 Protein Phosphorylation

Geng¹,

Tang²,

Li³

et al. 2011

Journal of Biological Chemistry

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Allyl isothiocyanate (AITC) occurs in many commonly consumed cruciferous vegetables and exhibits significant anti-cancer activities. Available data suggest that it is particularly promising for bladder cancer prevention and/or treatment. Here, we show that AITC arrests human bladder cancer cells in mitosis and also induces apoptosis. Mitotic arrest by AITC was associated with increased ubiquitination and degradation of ␣-and ␤-tubulin. AITC directly binds to multiple cysteine residues of the tubulins. AITC induced mitochondrion-mediated apoptosis, as shown by cytochrome c release from mitochondria to cytoplasm, activation of caspase-9 and caspase-3, and formation of TUNEL-positive cells. Inhibition of caspase-9 blocked AITCinduced apoptosis. Moreover, we found that apoptosis induction by AITC depended entirely on mitotic arrest and was mediated via Bcl-2 phosphorylation at Ser-70. Pre-arresting cells in G 1 phase by hydroxyurea abrogated both AITC-induced mitotic arrest and Bcl-2 phosphorylation. Overexpression of a Bcl-2 mutant prevented AITC from inducing apoptosis. We further showed that AITC-induced Bcl-2 phosphorylation was caused by c-Jun N-terminal kinase (JNK), and AITC activates JNK. Taken together, this study has revealed a novel anticancer mechanism of a phytochemical that is commonly present in human diet.Allyl isothiocyanate (AITC) 2 is a naturally occurring compound that possesses both antimicrobial and anticancer activities. Many commonly consumed cruciferous vegetables are rich sources of AITC, such as mustard, horseradish, wasabi, and cabbage. Its bactericidal and fungicidal activities were demonstrated against a variety of pathogens, and its anticancer activities were shown in both cultured cancer cell lines and animal tumor models (1). Bioavailability of AITC is extremely high; nearly 90% of orally administered AITC is absorbed (1). Although available evidence indicates that the anticancer activity of AITC is neither cell-nor tissue-specific, we have recently shown that AITC is selectively delivered to bladder tissue through urinary excretion and potently inhibits cancer development and muscle invasion in an orthotopic rat bladder cancer model (2). Moreover, an AITC-rich mustard seed powder also strongly inhibited bladder cancer development and muscle invasion in vivo (3). Thus, AITC is highly promising for bladder cancer prevention and/or treatment. These results are also consistent with epidemiological studies showing an inverse association between consumption of cruciferous vegetables and bladder cancer risk (4, 5). In light of these findings, this study focuses on human bladder cancer cells.Previous studies have shown that AITC causes cell cycle arrest and apoptosis in cancer cell lines of different tissue origins in vitro and in several tumor xenograft models in vivo, and it modulates many genes and proteins involved in cancer cell survival and proliferation (1). In our recent studies, both AITC and the AITC-rich mustard seed powder mentioned above arrested bladder cancer cells in G 2 /M ...

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“…Several observations made during the first half of the 20 th century provided valuable contributions towards our current understanding of the processes involved in vascular and neurogenic inflammation. It is now well recognized that the glucosinolates and isothiocyanates present in mustard seeds and its oils are involved in their cancer preventive effects, and that these components are orally absorbed from vegetables as well (Bhattacharya et al, 2010;Shapiro et al, 1998). Furthermore, realization of the fact that edible mustard oil is a rich source of polyunsaturated fatty acids and phytosterols has also led to the speculation that it could also have cardio-protective effects and other health benefits.…”

Section: Pharmacology and Toxicologymentioning

confidence: 99%

Therapeutic potentials of Brassica juncea: an overview

Kumar¹,

Thakur²,

Barothia³

et al. 2011

TANG [HUMANITAS MEDICINE]

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Diverse medicinal uses of different types of products obtainable from Brassica juncea have been known for centuries. Most such traditionally known uses of the plant have been centered on its seeds and oils obtainable from them. During more recent decades diverse bio-active molecules and their therapeutically interesting pharmacological properties of its green edible leaves have also been described, and they are now often considered to be effective substitutes for other so called "healthy" Brassica vegetables. However, little concentrated effort has yet been made to obtain a pharmacologically better defined phytopharmaceutical from this easily cultivable plant of commercial interest in many underdeveloped and developing countries. The main aim of this overview is to point out some possibilities for designing and developing such products from the plant for combating the rapidly spreading obesity epidemic in the developed countries and some other countries. Efforts to achieve such goals could as well be an economically more feasible, and culturally more acceptable, starting point for better understanding the potential health benefits of other vegetarian foods.

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Allyl isothiocyanate-rich mustard seed powder inhibits bladder cancer growth and muscle invasion

Cited by 85 publications

References 17 publications

Effect of allyl isothiocyanate on the viability and apoptosis of the human cervical cancer HeLa cell line inï¿½vitro

Effect of allyl isothiocyanate on the viability and apoptosis of the human cervical cancer HeLa cell line inï¿½vitro

Allyl Isothiocyanate Arrests Cancer Cells in Mitosis, and Mitotic Arrest in Turn Leads to Apoptosis via Bcl-2 Protein Phosphorylation

Therapeutic potentials of Brassica juncea: an overview

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