Aloe ferox

Viljoen, Alvaro; Chen, Weiyang; Mulaudzi, Nduvho; Fouché, Gerda

doi:10.1016/b978-0-323-99794-2.00006-4

Cited by 3 publications

(1 citation statement)

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“…The new barcoding tool is applicable to a wide range of species, as compared to authentication techniques such as chemical fingerprinting which achieve high resolution but tend to be narrowly focused on few species (e.g. Aloe ferox 29 ). While the benefits of DNA-based diagnostics are well established, successful protocols are contingent on comprehensive and taxonomically verified reference sequence datasets.…”

Section: Discussionmentioning

confidence: 99%

An updated DNA barcoding tool forAloe veraand related CITES-regulated species

Woudstra,

Rees,

Rakotoarisoa

et al. 2024

Preprint

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DNA barcoding has revolutionised the identification of illegally traded material of endangered species as it overcomes the lack of resolution encountered with morphological identification. Nonetheless, in recently evolved and highly diverse clades, such as the relatives ofAloe vera, the lack of interspecific sequence variation in standardised markers compromises the barcoding efficacy. We present a new DNA barcoding tool using 189 nuclear markers, optimised for aloes (Asphodelaceae, Alooideae). We built a comprehensive sequence reference dataset from taxonomically verified sources for >300 species and validated its reliability for identification using phylogenomic inference. Seven anonymised samples from verified botanical collections and ten plants seized at London Heathrow Airport were correctly identified to species level, including a critically endangered species from Madagascar. Commercially purchased samples were confirmed to be the species as advertised. An accurate, reliable DNA barcoding method for aloe identification introduces new assurance to regulatory processes for endangered plants in trade.

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Section: Discussionmentioning

confidence: 99%