Aloe Vera/Collagen Mixture Induces Integrin α1β1 and PECAM-1Genes Expression in Human Adipose-Derived Stem Cells

Sigaroodi, Faraz; Shafaei, Hajar; Karimipour, Mohammad; Dolatkhah, Mohammad Amin; Delazar, Abbas

doi:10.15171/apb.2019.077

Cited by 9 publications

(5 citation statements)

References 38 publications

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“…The right testicles of animals were harvested for the assessment of the expression rate of SF‐1, CatSper‐1, 3β‐HSD, CatSper‐2, LHCGR, and 17β‐HSD3 genes. The RNA contents of spermatozoa were extracted by the TRIzol reagent (Invitrogen, Paisley, UK) based on the protocol established by our previous research (Sigaroodi et al, 2019; Delkhosh et al, 2021). The DNase I enzyme (Invitrogen Life Technologies, Carlsbad, CA, USA) was utilized to eliminate possible DNA contamination in extracted RNA samples, according to the manufacturer's instructions.…”

Section: Evaluation Of Sperm Parametersmentioning

confidence: 99%

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

et al. 2022

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Varicocele (VCL) is a pathological dilation of the venous pampiniform plexus of the spermatic cord and is also classified as male factor infertility. The current experiment aimed to examine the protective effect of Fumaria parviflora (FP), as a powerful antioxidant, against reproductive damage induced by VCL. In this experimental study, 32 male rats were randomly allocated into four groups, namely sham (simple laparotomy without additional intervention), FP (healthy rats administered 250 mg/kg FP), VCL + FP (underwent VCL and received 250 mg/kg FP), VCL (underwent VCL without receiving any treatment). The results showed that the number of Sertoli and germ cells were markedly reduced in the VCL group in comparison to the FP‐treated and sham groups. The VCl + FP group had significantly higher serum levels of testosterone (T), FSH, and LH hormones than the VCL group. The quality and motility of spermatozoa were reduced in the VCL group compared with other groups (p ≤ 0.05). Moreover, our findings demonstrated that the administration of FP considerably enhanced the mRNA levels of CatSper‐1 and ‐2, SF‐1, 3β‐HSD, 17β‐HSD3, LHCGR, and FSHR (p ≤ 0.05). Based on the obtained results, treatment with FP is capable of preventing testicular dysfunction and elevating the concentration of hormones and some crucial genes, such as CatSper1 and 2, SF‐1, 3β‐HSD, 17β‐HSD3, LHCGR, and FSHR that contribute to the spermatogenesis process.

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Section: Evaluation Of Sperm Parametersmentioning

confidence: 99%

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

et al. 2022

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“…Among natural polymers, proteins such as collagen [2] and polysaccharides such as chitosan [3] may be very valuable due to their intrinsic bioactivity, biocompatibility and biodegradability [4]. When these materials are located in a defective area, they may promote the migration of cells and their integration to the surrounding environment, thus forming an extracellular matrix (ECM) and improving the structure of the repaired tissue [5].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and Biological Performance of Aloe Vera-Incorporated Native Collagen Films

et al. 2020

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Collagen was obtained from porcine skin by mechanical pretreatments with the aim of preserving the triple helix structure of native collagen, which was indirectly corroborated by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR) results. Moreover, aloe vera (AV), with inherent biological properties, was incorporated into collagen film formulations, and films were prepared by compression and characterized to assess their suitability for biomedical applications. SEM images showed that the fibrillar structure of collagen changed to a rougher structure with the addition of AV, in accordance with the decrease in the lateral packaging of collagen chains observed by XRD analysis. These results suggested interactions between collagen and AV, as observed by FTIR. Considering that AV content higher than 20 wt % did not promote further interactions, this formulation was employed for biological assays and the suitability of AV/collagen films developed for biomedical applications was confirmed.

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“…), the plant approved and cultivated in the Greenhouse of the Faculty of Agriculture, Tabriz University, was used in this study. The new plant was used according to a previously published method (23,24).…”

Section: Preparation Of Aloe Vera Gelmentioning

confidence: 99%

The Role of Aloe vera in Inhibiting the P53 Protein Expression and Enhancing the HT29 Colon Cancer Cells Proliferation

Hesari,

Shafaei,

Khamnei

et al. 2023

Zahedan J Res Med Sci

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Background: Aloe vera is one of the most widely recognized herbs considered a valid treatment option for its wound healing and anti-inflammatory properties. However, there is insufficient evidence regarding the effectiveness of its anti-tumor activity on colon cancer. Objectives: In this study, therefore, we investigated the effect of Aloe vera on the proliferation and survival of the HT29 colon cancer cell line. Methods: The effect of processed Aloe vera gel (PAG) on colon carcinogenesis was examined using low glucose Dulbecco’s modified Eagle’s medium (DMEM) with 10% serum and 1% penicillin-streptomycin in the HT29 cells. Then, we obtained the expression levels of P53 and BCL2 using the immunocytochemical (ICC) method. To determine the viability of the HT29 cells, we also performed the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The MTT assay showed that the HT29 cells were viable in 5%, 10%, 15%, and 20% of Aloe vera gel (AVG). However, these cells had a more obvious proliferation in the 10% Aloe vera group than in the control group. In the 10% concentration group, mitotic cells were evident in the center of the colonies. The P53 protein expression decreased, but the BCL2 protein expression remained unchanged in the 10% group compared to the control group. Conclusions: The results, therefore, indicated that in the 10% Aloe vera concentration, the expression levels of P53, known as a tumor suppressor protein in colon cancer, decreased in HT29. Our results also showed that the application of Aloe vera for its anti-tumor activity in colon cancer should be further evaluated.

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Aloe Vera/Collagen Mixture Induces Integrin α1β1 and PECAM-1Genes Expression in Human Adipose-Derived Stem Cells

Cited by 9 publications

References 38 publications

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

Physicochemical and Biological Performance of Aloe Vera-Incorporated Native Collagen Films

The Role of Aloe vera in Inhibiting the P53 Protein Expression and Enhancing the HT29 Colon Cancer Cells Proliferation

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