Aloenxertos ósseos caninos diferentemente preservados

Carlo, Ricardo Junqueira Del; Galvão, Simone Rezende; Vilória, Marlene Isabel Vargas; Souza, Tamara Daiane de; Maia, A Ferraro

doi:10.4322/rbcv.2015.151

Cited by 12 publications

(17 citation statements)

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“…Glycerol may have a bacteriostatic activity, as demonstrated in studies by Del Carlo et al (1999) instead of a bactericidal activity, as mentioned by other authors (Gioso et al, 2002, Giovani et al, 2006.…”

Section: Discussionmentioning

confidence: 51%

“…Storage in glycerol for 30 days was an effective technique for the destruction of the parasite, which suggests that bone grafts after this processing would not transmit infection of L. infantum to the transplant recipient dog. Bacteriostatic or bactericidal effects of glycerol are reported in the literature (Del Carlo et al, 1999;Giovanni et al, 2006). This antimicrobial capacity is because glycerol is a fast fixative and dehydrating agent, acting as a powerful antiseptic (Alvarenga, 1992).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the presence of Leishmania infantum in bone stored in glycerol or sterilized by autoclave for cortical bone grafting

Lopes

Silva

Amorim

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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Evaluation of the presence of ABSTRACTThis study was performed to evaluate the effects of autoclaving and storage in 85% glycerol on cortical bone from dogs infected with Leishmania sp. We used 42 cadavers with leishmaniasis. The dogs were evaluated for the presence of Leishmania sp. in culture of bone marrow and by culturing cortical bone. From the infected animals, we harvested 42 diaphysis of the right femur for culture of cortical bone before and after autoclaving or storage in glycerol. There was no significant difference in growth of the parasite in culture of bone marrow or cortical bone. There was no growth of Leishmania sp. in culture of samples after autoclaving or storage in glycerol. Both treatments were effective in preventing the growth of the parasite in vitro, so it was considered viable for grafting.Keywords: bone graft, canine visceral leishmaniasis, glycerol, sterilization Palavras-chave: enxerto ósseo, leishmaniose visceral canina, glicerol, esterilização RESUMO Este estudo teve por objetivo avaliar os efeitos da esterilização em autoclave e do armazenamento em glicerol a 85% no osso cortical de cães infectados por

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Evaluation of the presence of Leishmania infantum in bone stored in glycerol or sterilized by autoclave for cortical bone grafting

Lopes

Silva

Amorim

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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“…A meniscectomia total e a substituição dos meniscos por aloenxertos são procedimentos freqüentemente realizados quando outras opções para reparo ou reconstrução do menisco não puderem ser utilizadas (Rijk 2004, Reckers et al 2005 Um meio de preservação frequentemente utilizado para próteses biológicas é a glicerina a 98%, mantida em temperatura ambiente. Ela apresenta como vantagens o baixo custo e propriedade antisséptica, atuando como bactericida e fungicida (Alvarenga 1992, Del Carlo et al 1999, Filgueiras et al 2007, além de reduzir a antigenicidade do tecido nela conservado e preservar a textura (Pigossi 1967). Guimarães et al (2007) em um estudo com membranas biológicas preservadas em glicerina a 98% por diferentes períodos, relataram a ocorrência de retração das fibras colágenas e desorganização tecidual, mas com mínimas alterações na integridade tecidual.…”

Section: Introductionunclassified

Avaliação das fibras colágenas de meniscos frescos e preservados em glicerina: estudo experimental em coelhos (Oryctolagus cuniculus)

Vilela

Carlo²,

Silva³

et al. 2010

Pesq. Vet. Bras.

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In the present study was evaluated the effect of 98% glycerin on the collagen fibers, tissue architecture and size of medial meniscuses of New Zealand rabbits. The animals were separated into three groups: (1) Group MF of fresh meniscus, (2) Group MG of meniscus preserved in glycerin for 30 days, and (3) Group MR of meniscus preserved in glycerin for 30 days and rehydrated in NaCl 0.9% for 12 hours. Histological sections were stained with sirius red for identification of collagen types, which were examined with a polarized light microscope. The total collagen concentration and the fiber arrangement were evaluated. Group MF presented higher Type I collagen concentration and lower Type III collagen concentration when compared with Group MG and MR. This fact is due to the water loss and consequent reduction in size and subsequent retraction of the collagen fibers of the meniscus from these groups, caused by dehydration. This may have occurred because those Type I fibers, thicker and in larger quantities, become more evident than Type III collagen fibers, which are more slender and fragile (fibrils). In the three groups studied, the collagen fibers presented themselves in a circumference form, interposed by radially oriented fibers. All of the meniscuses from Group MF presented fibers arranged obliquely, while in the treated groups, a slight disorganization of collagen fibers could be observed in some areas, what corresponds to 42.8% and 14.3% of the meniscuses, respectively. The MG group presented a significant decrease (p<0.05) in size if compared with the MF group. In the MR group, 85.7% of the meniscuses went back to the original size after rehydratation. The 98% glycerin is effective in preserving the meniscus, following rehydratation, maintaining size, structural architecture, integrity and percentage of collagen of the meniscus preserved similar to the fresh one.INDEX TERMS: Allograft, articular cartilage, meniscus.RESUMO.-No presente estudo foi avaliado o efeito da glicerina 98% sobre as fibras colágenas, arquitetura tecidual e o tamanho de meniscos mediais de coelhos da raça Nova Zelândia. Os meniscos foram separados em três grupos: (1) Grupo MF com meniscos frescos (grupo controle), (2) Grupo MG com meniscos preservados em glicerina 98% por 30 dias, e (3) Grupo MR com meniscos preservados em glicerina 98% por 30 dias e reidratados em NaCl 0,9%, por 12 horas. Os cortes histológicos fo-

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“…DEL CARLO et al (1999) e (2007) recomendaram que o enxerto preservado em glicerina deve ser imerso em solução fisiológica estéril acrescida de antibiótico para garantir rehidratação, prevenir contaminações e remover o meio de conservação, antes da enxertia. KRAUSPENHAR (2003) recomendou que o período mínimo de preservação dos enxertos é de 30 dias, pois a eliminação de bactérias gram negativas neste meio pode requerer até 27 dias.…”

Section: Introductionunclassified

Propriedades mecânicas de meniscos frescos de coelhos e preservados em glicerina 98%

et al. 2010

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Aloenxertos ósseos caninos diferentemente preservados

Cited by 12 publications

References 1 publication

Evaluation of the presence of Leishmania infantum in bone stored in glycerol or sterilized by autoclave for cortical bone grafting

Evaluation of the presence of Leishmania infantum in bone stored in glycerol or sterilized by autoclave for cortical bone grafting

Avaliação das fibras colágenas de meniscos frescos e preservados em glicerina: estudo experimental em coelhos (Oryctolagus cuniculus)

Propriedades mecânicas de meniscos frescos de coelhos e preservados em glicerina 98%

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