Aloin A prevents ulcerative colitis in mice by enhancing the intestinal barrier function via suppressing the Notch signaling pathway

Jiang, Hui; Shi, Gaofeng; Fang, Yu-Xi; Liu, Youqian; Wang, Qi; Zheng, Xiaoya; Zhang, Dong-Jian; Zhang, Jian; Yin, Zhiqi

doi:10.1016/j.phymed.2022.154403

Cited by 15 publications

(11 citation statements)

References 40 publications

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“…26 MUC2, distributed in small intestine and large intestine, is the main component of the intestinal mucus layer, and the contents of MUC2 can strengthen the intestinal barrier. 27 In this study, our data indicated that LPS significantly increased the DAO activity and the D-LA content in serum and decreased the mRNA expression of tight junction protein related genes ZO-1, occludin, claudin-1, MUC2 and JAM2 in jejunum of mice. However, the naringin treatment mitigated these effects induced by LPS.…”

Section: Papersupporting

confidence: 53%

Naringin mitigates LPS-induced intestinal barrier injury in mice

et al. 2023

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Section: Papersupporting

confidence: 53%

Naringin mitigates LPS-induced intestinal barrier injury in mice

et al. 2023

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“…These results indicate that RR5, FAI10, MO4, RRP6, and RS11 play pivotal roles in the treatment of IO with TFXXD. Recent investigations have revealed the potential of RR5 to fortify the integrity of the intestinal barrier and reduce colon permeability through suppression of the Notch signaling pathway 34 . Additionally, FAI10 has been demonstrated to alleviate lipopolysaccharide (LPS) induced intestinal damage by mitigating inflammation, enhancing antioxidant mechanisms, and improving tight junctions.…”

Section: Discussionmentioning

confidence: 99%

“…Recent investigations have revealed the potential of RR5 to fortify the integrity of the intestinal barrier and reduce colon permeability through suppression of the Notch signaling pathway. 34 mechanism by which MO regulates GI motility primarily centers on its impact on GI smooth muscle and Cajal cells, as well as on its modulation of GI hormone secretion. 12 RRP6, one of the bioactive ingredients of RRP, may facilitate mucosal tissue repair by stimulating intestinal epithelial cell proliferation and averting cell death through the upregulation of TGF-β.…”

Section: Molecular Dockingmentioning

confidence: 99%

Identifying the active compounds and mechanism of action of TongFu XieXia Decoction for treating intestinal obstruction using network pharmacology combined with ultra‐high performance liquid chromatography‐quadrupole‐orbitrap mass spectrometry

Ma,

Wu,

Luo

et al. 2023

Rapid Comm Mass Spectrometry

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RationaleTongFu XieXia Decoction (TFXXD), a formulation rooted in traditional Chinese medicine and optimized through clinical practice, serves as an advanced version of the classic Da Cheng Qi decoction used for treating intestinal obstruction (IO), demonstrating significant therapeutic efficacy. However, due to the intricate nature of herbal compositions, the principal constituents and potential mechanisms of TFXXD have yet to be clarified. Accordingly, this study seeks to identify the active compounds and molecular targets of TFXXD, as well as to elucidate its anti‐IO mechanisms.MethodsQualitative identification of the principal constituents of TFXXD was accomplished using ultra‐high preformance liquid chromatography‐quadrupole‐orbitrap mass spectrometry (UPLC‐Q‐Orbitrap‐MS/MS) analysis. PharmMapper facilitated the prediction of potential molecular targets, whereas protein–protein interaction analysis was conducted using STRING 11.0. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the Metascape database. A “compounds‐target‐pathway” network was meticulously constructed within Cytoscape 3.8.2. Finally, molecular docking studies were performed to investigate the interactions between the core target and the crucial compound.ResultsUPLC‐Q‐Orbitrap‐MS/MS analysis identified 65 components with high precision and sensitivity. Furthermore, 64 potential targets were identified as integral to TFXXD bioactivity in IO treatment. Gene Ontology enrichment analysis revealed 995 distinct biological functions, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 143 intricate signaling pathways.ConclusionMolecular docking studies substantiated the substantial affinity between the TFXXD bioactive constituents and their corresponding targets in the context of IO. TFXXD exerts its therapeutic efficacy in IO through a multifaceted interplay between multiple compounds, targets, and pathways. The integration of network pharmacology with UPLC‐Q‐Orbitrap‐MS/MS has emerged as a promising strategy to unravel the intricate web of molecular interactions underlying herbal medicine. However, it is imperative to emphasize the necessity for further in vivo and in vitro experiments.

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“…Aloin A 21 is an anthraquinone glycoside extracted from the secretion of Aloe vera leaves and has anti-inflammatory, anti-bacterial, antioxidant, anti-viral, and anti-cancer pharmacological effects [ 168 ]. Aloin A 21 (25 and 50 mg/kg) can prevent DSS-induced colitis by enhancing intestinal barrier function through inhibition of the Notch signaling pathway [ 95 ].…”

Section: Anti-inflammatory Effects Of Glycosides In a Model Of Ucmentioning

confidence: 99%

“…Molecules 2023, 28, x FOR PEER REVIEW 19 of 33 3, aloin A 21, linarin 5, vitexin 6, and naringin 7 can improve the expression of TJ proteins and mucin proteins in intestinal mucosa and inhibit the increase of intestinal mucosal permeability, exerting anti-inflammatory effects[64,67,71,72,74,95]. The above suggests that intestinal epithelial barrier function plays an important role in the pathogenesis of UC (Figure4).…”

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confidence: 96%

Glycosides as Potential Medicinal Components for Ulcerative Colitis: A Review

et al. 2023

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Ulcerative colitis (UC) is a chronic, non-specific disease of unknown etiology. The disease develops mainly in the rectum or colon, and the main clinical symptoms include abdominal pain, diarrhea, and purulent bloody stools, with a wide variation in severity. The specific causative factors and pathogenesis of the disease are not yet clear, but most scholars believe that the disease is caused by the interaction of genetic, environmental, infectious, immune, and intestinal flora factors. As for the treatment of UC, medications are commonly used in clinical practice, mainly including aminosalicylates, glucocorticoids, and immunosuppressive drugs. However, due to the many complications associated with conventional drug therapy and the tendency for UC to recur, there is an urgent need to discover new, safer, and more effective drugs. Natural compounds with biodiversity and chemical structure diversity from medicinal plants are the most reliable source for the development of new drug precursors. Evidence suggests that glycosides may reduce the development and progression of UC by modulating anti-inflammatory responses, inhibiting oxidative stress, suppressing abnormal immune responses, and regulating signal transduction. In this manuscript, we provide a review of the epidemiology of UC and the available drugs for disease prevention and treatment. In addition, we demonstrate the protective or therapeutic role of glycosides in UC and describe the possible mechanisms of action to provide a theoretical basis for preclinical studies in drug development.

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Aloin A prevents ulcerative colitis in mice by enhancing the intestinal barrier function via suppressing the Notch signaling pathway

Cited by 15 publications

References 40 publications

Naringin mitigates LPS-induced intestinal barrier injury in mice

Naringin mitigates LPS-induced intestinal barrier injury in mice

Identifying the active compounds and mechanism of action of TongFu XieXia Decoction for treating intestinal obstruction using network pharmacology combined with ultra‐high performance liquid chromatography‐quadrupole‐orbitrap mass spectrometry

Glycosides as Potential Medicinal Components for Ulcerative Colitis: A Review

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