Alpha-1 antitrypsin is elevated in exhaled breath condensate and serum in exacerbated COPD patients

Koczulla, A. Rembert; Noeske, Sarah; Herr, Christian; Koepke, Janine; Jörres, Rudolf A.; Nell, Christoph; Schmid, Severin; Vogelmeier, Claus; Bals, Robert

doi:10.1016/j.rmed.2011.06.015

Cited by 31 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fluid was stored at -80uC for ELISA-based analysis of TIMP-1 and MMP-9 (R&D Systems, Minneapolis, MN, USA), which were conducted according to the manufacturer's suggested routine procedure. a 1 -AT was measured by ELISA as described previously [22]. The sensitivity of the ELISAs for TIMP-1, MMP-9 and a 1 -AT was 30 pg?mL -1 , 30 pg?mL -1 and 40 pg?mL -1 , respectively.…”

Section: Pharyngeal Washing Fluidmentioning

confidence: 99%

Detection of obstructive sleep apnoea by an electronic nose

et al. 2012

Self Cite

View full text Add to dashboard Cite

Diagnosis of obstructive sleep apnoea syndrome (OSAS) is technically demanding, costintensive and time-consuming. The measurement of volatile organic compounds by an electronic nose is an innovative method that determines distinct molecular patterns of exhaled breath in different patient groups. We addressed the following questions: What is the diagnostic accuracy of an electronic nose in the detection of OSAS and the ability to detect effects of standard therapy in patients with OSAS? Are these results related to changes in distinct markers of airway inflammation and extracellular remodelling?We included 40 OSAS patients and 20 healthy controls. Exhaled breath of all participants was analysed using the Cyranose 320 electronic nose. Pharyngeal washings were performed to sample the upper airway compartment. For statistical analysis linear discriminant analysis was employed.We identified a linear discriminant function separating OSAS from control (p,0.0001). The corresponding area under the receiver-operating curve was 0.85 (95% CI 0.75-0.96; sensitivity 0.93 and specificity 0.7). In pharyngeal washing fluids of OSAS patients, we observed higher levels of a 1 -antitrypsin and markers of extracellular remodelling compared to controls.The electronic nose can distinguish between OSAS patients and controls with high accuracy. @ERSpublicationsThe electronic nose can distinguish between OSAS patients and controls with high accuracy

show abstract

Section: Pharyngeal Washing Fluidmentioning

confidence: 99%

Detection of obstructive sleep apnoea by an electronic nose

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…49 The "exhaled breath condensate" (rich in water vapors and several active molecules) has the same significance and permits the determination of a large number of cytokines (TNF-alpha, IL-6, IL-8, isoprostane, leukotrienes -moderators of inflammation and oxidative stress in COPD). 50,51 …”

Section: Markers In Exhaled Air In Copd Exacerbationmentioning

confidence: 99%

Inflammatory Biomarkers in Chronic Obstructive Pulmonary Disease

Comes

Ianoși

Jimborean

2016

Journal of Interdisciplinary Medicine

View full text Add to dashboard Cite

Chronic obstructive lung disease (COPD) is a severe progressive disease associated with high morbidity and mortality. Early diagnosis and correct treatment improve the symptoms, quality of life and survival in COPD. Exacerbations of the disease are acute events that cause worsening of COPD symptoms (dyspnea, cough and/or sputum) and may require modification of stable COPD therapy. COPD exacerbations add inflammation, damage the quality of life, deteriorate the lung function, increase mortality and associate high socio-economic costs. Accurate early prediction of exacerbation and mortality risk facilitates patient selection upon risk, in order to provide appropriately targeted early treatment. The risk of having frequent exacerbations is clearly demonstrated by recognized studies in patients with specific criteria: previous exacerbation in the last year, decrease in FEV1s, increase in the score of St. George Questionnaire (life quality decline), high levels of several inflammatory biomarkers, such as neutrophils, C-reactive protein (CRP), fibrinogen, pro-calcitonin, eosinophils, IL-6, IL-8, chemokine ligand 18 (CCL-18/PARC), surfactant protein D (SP-D). Simultaneously elevated levels of CRP, fibrinogen and leukocyte count in COPD patients were associated with an increased risk for exacerbations. At the same time, elevated levels of the three biomarkers are associated with an increased risk of major comorbidities in COPD. Biomarker detection may be an additional tool for assessment and management of COPD comorbidities. Detection of pathologic levels of inflammatory biomarkers improves the ability to predict the risk mortality in COPD alongside with BODE index (BMI, obstruction in lung function, dyspnea scale, 6-minute walk test) and may provide a targeted treatment.

show abstract

“…Использование биомаркеров при ХОБЛ может помочь в определении различных фенотипов заболевания, долгосрочных прогнозов или ответа на терапевтические воздействия [3,4]. До сих пор спе цифические биомаркеры ХОБЛ не определены [5].…”

Section: обзорыunclassified

“…К предполагаемым биомаркерам ХОБЛ относят нейт рофилез мокроты, который указывает на ухудшение функции легких [2], а также эозинофилию мокроты, которая является предиктором ответа на лечение глюкокортикостероидами (ГКС) [6,7]. Другие опре деляемые биомаркеры могут помочь при диагности ке и мониторинге обострений ХОБЛ [4,5,8,9].…”

Section: обзорыunclassified

“…В КВВ также определяются другие молекулы, вклю чая малоновый диальдегид [56], эритропоэтин и TNF α [57], α 1 антитрипсин [4], интерферон γ и миелопероксидазу [58], нейтрофильные протеоли тические ферменты -нейтрофильную эластазу, про теиназу 3 и катепсин G [36], различные пурины, в т. ч. аденозин [11,59], микроэлементы и токсич ные металлы [60], а также другие маркеры, которые дают важную информацию о патогенезе ХОБЛ.…”

Section: другие молекулы и веществаunclassified

See 1 more Smart Citation