Alpha-amylase inhibition kinetics by caulerpenyne

Abstract

Many algae have important secretions which are generally used for defensive purposes. These secretions have caught the attention of a number of researchers, as to whether or not these metabolites can be used in medical research. Among these metabolites, caulerpenyne (CYN) which is the main metabolite of the Caulerpa species, has had an important place in Caulerpa research since the beneficial effects related to its determined properties (such as cytotoxic, antiviral, antiproliferative and apoptotic) have been demonstrated in many scientific reports. In the present study, the inhibitory effect of CYN isolated from C. prolifera on alpha-amylase was investigated. The inhibition experiments were done with CYN by a spectrophotometric determination method. In order to evaluate the type of inhibition a Lineweaver-Burk plot was produced. The results obtained from enzyme kinetic studies exhibited a non-competitive type of inhibition, which is characterized by the difference of V max and K M from those of the free enzyme, of alpha-amylase in the presence of CYN. The present study showed that Caulerpa species may be a potential target for the production of diabetic drugs in the light of the results obtained for CYN.

Inhibition of xanthine oxidase by Caulerpenyne from Caulerpa prolifera

“…It was presented in the previous reports that CYN, the main secondary metabolite of Caulerpa species, has some unique properties such as anticarcinogenic, antiproliferative, apoptotic and antiviral effects [16][17][18][19]. Moreover, the enzyme inhibitory effects of CYN towards some medically important enzymes like phospholipase A 2 , lipase, lipoxygenase and amylase have been shown by some researchers [21][22][23][24][25][26][27]. In the light of the knowledge above, the inhibitory effects of CYN on XOD enzyme activity were determined in this study.…”

“…Due to the results of this study CYN can be classified as a good inhibitor for XOD and therefore, it can be recommended as a potential drug for XOD related diseases after in vivo studies. Unfortunately, CYN inhibits not only XOD but also phospholipase A 2 [21], lipoxygenase [22,23], pancreatic lipase [24,25] and alpha amylase [26,27]. Many drugs, which are currently used, act similarly and by reacting with different pathways they may have severe side effects.…”

“…The extraction and purification of CYN from C. prolifera were conducted according to previously described procedures [23,27,29]. The purified CYN was analysed by reverse-phase HPLC and 1 H NMR spectroscopy in order to determine its purity.…”

“…There are few studies in the literature which evaluate the enzyme inhibitory potential of CYN and its derivatives. It inhibits phospholipase A 2 [21], lipoxygenase [22,23], pancreatic lipase [24,25] and α-amylase [26,27].…”

Photoredox Catalysis of Intramolecular Cyclizations with a Reusable Silica‐Bound Ruthenium Complex

“…Following up reports on the inhibitory activity of CYN ( 1 ) on microtubule protein polymerization, first synthetic CYN analogues in form of colchicine hybrids were generated, however with limited activity 3. CYN ( 1 ) itself is also active as inhibitor of telomerase,4 amylase,5 xanthine oxidase6 and plant lipoxygenases (LOs) 7. We reported that CYN ( 1 ) inhibits soybean LO with an IC 50 value of 5.1 μ M in an uncompetitive manner 7b.…”