Alpha-amylase inhibitory activities of ascidians in the treatment of diabetes mellitus

Prabhu, Antonyraj Selva; Ananthan, G.

doi:10.3329/bjp.v9i4.20017

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…The abdominal distention, flatulence, meteorism, and possibly diarrhea are main adverse effects of acarbose [8]. The exploration for safer, specific, and effective hypoglycemic agents has sustained to be a crucial space of inquiry with herbal extracts from promptly offered traditional medicinal plants, providing the nice potential for discovery of new antidiabetic drugs [9]. Schleichera oleosa (Lour) Oken, usually recognized as Lac tree or Kusum, is a forest tree class of thetropical and subtropical region.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Studies to Assess the Antidiabetic Potential of Schleichera Oleosa (Lour) Oken Leaves

Muthukrishnan

Sivakkumar

2017

Asian J Pharm Clin Res

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Objective: The aim of this research is to establish the antidiabetic properties of sequential extracts of Schleichera oleosa (lour) Oken leaves thru α-amylase and α-glucosidase inhibitory assay.Methods: The extracts of S. oleosa (Lour) Oken were prepared by continuous hot percolating the dried powder of the plant leaves. The various solvents were used for the extraction and qualitative assay for the phytochemical test using standard protocols. Different concentration (1, 2, 4, 6, 12, 25, and 50 mg/ml) of sequential extracts of S. oleosa leaves were used to assess the in vitro α-amylase and α-glucosidase inhibitory assay by Bernfeld and Apostolidis method.Results: In the α-amylase assay, the ethanolic extract produced 52.76% inhibition at 4 mg/ml concentration, but in ethyl acetate and aqueous extracts case 50% inhibition attained only at the concentration of 50 mg/ml, and acarbose 0.9 mg/ml was found 89.24% inhibition. In the α-glucosidase assay, the all extracts show the decent inhibitory effect in 50 mg/ml. The ethanolic and aqueous extracts exhibited a higher inhibitory effect 72.64% and 59.44% than other extracts at the concentration of 50 mg/ml, respectively, while acarbose 0.9 mg/ml was producing 86.24% inhibition. This result indicates that the inhibition of ethanolic and aqueous extracts on the activity from α-amylase and α-glucosidases is much more potent than that of other extracts.Conclusion: This study revealed that ethanolic and aqueous extracts showed the high content of polyphenols and flavonoids, which was blamed for the α-amylase and α-glucosidases inhibition. Hence, it deserved to elucidate specific components and to evaluate the antidiabetic effect using in vivo animal model.

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Section: Introductionmentioning

confidence: 99%

In Vitro Studies to Assess the Antidiabetic Potential of Schleichera Oleosa (Lour) Oken Leaves

Muthukrishnan

Sivakkumar

2017

Asian J Pharm Clin Res

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“…These are enzymes responsible for breaking α , 1, 4 bonds in complex carbohydrate [ 44 ]. The inhibition of these enzymes delays the breakdown of carbohydrate which leads to reduced blood glucose [ 45 , 46 ]. The findings of this study indicated that U. chamae and its fractions ethyl acetate, chloroform, and ethanol caused α -amylase and α -glucosidase inhibition.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic Effects of the Ethanolic Root Extract ofUvaria chamaeP. Beauv (Annonaceae) in Alloxan-Induced Diabetic Rats: A Potential Alternative Treatment for Diabetes Mellitus

Emordi

Agbaje

Oreagba

et al. 2018

Advances in Pharmacological Sciences

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Diabetes mellitus has been a menace to mankind from time immemorial. However, a natural product such as U. chamae P. Beauv (Annonaceae) offers alternative treatment for diabetes mellitus. The study aimed at evaluating antidiabetic activity of the ethanolic root extract of U. chamae in alloxan-induced diabetic rats. Diabetes was induced in Sprague Dawley rats after overnight fast with 150 mg/kg alloxan intraperitoneally. After 72 h, those with plasma glucose levels >200 mg/dl were classified as diabetic. Five diabetic rats in each group were treated daily for 14 days orally with 100, 250, and 400 mg/kg of the extract, glibenclamide (71 µg/kg) and pioglitazone (429 µg/kg), respectively, while another group was untreated. Control received 0.5 ml of Acacia senegal. Effects of extract on glucose, other biochemical, and hematological parameters were evaluated. α-amylase and α-glucosidase inhibitory activities of extract and its fractions were also evaluated. Percentage inhibition and IC50 values were determined. Diabetic control was achieved on the 7th day of the study with 100, 250, and 400 mg/kg of the extract showing glucose reduction of 72.14%, 78.75%, and 87.71%, respectively. The HDL-cholesterol levels of diabetic rats treated with extracts were significantly increased. Extract and its fractions caused α-amylase and α-glucosidase inhibition. Histologically, pancreas of diabetic rats treated with extract showed regenerated islet cells which were not seen in rats treated with glibenclamide and pioglitazone. This study showed that U. chamae has antidiabetic activity which may be through α-amylase and α-glucosidase inhibition and regeneration of pancreatic beta cells. Also, it may reduce the risk of cardiovascular disease by increasing HDL-cholesterol levels.

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“…Alpha-amylase inhibitory activities of ascidians (Prabhu and Ananthan, 2014), Gossypium arboreum (Kazeem et al, 2013), Newbouldia laevis (Kolawole and Akanji, 2013) and Lantana camara (Swamy and Sinniah, 2015) were reported.…”

Section: Discussionmentioning

confidence: 99%

Synthesis, characterization and evaluation of antidiabetic activity of novel indoline derivatives

Manoharan¹,

Kulanthai²,

Gnanavel³

et al. 2017

Bangladesh J Pharmacol

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Series of indoline derivatives were synthesized using N-(4-aminophenyl)indoline-1-carbothiamide as a precursor. The confirmation of synthesized compounds was done by 1H-NMR, 13C-NMR, LC-MS (ESI) and FT-IR. In vitro antidiabetic activity of synthesized indoline derivatives were examined by standard α-amylase inhibition assay. The compounds 4a (IC50 = 52.1 µg/mL) and 4b (IC50 = 57.7 µg/mL) showed potent α-amylase inhibition activity. The compounds 3a (IC50 = 62.2 µg/mL) and 3b (IC50 = 60.7 µg/mL) showed moderate antidiabetic activity.Video Clip of Methodology:19 min 21 sec <a href="https://www.youtube.com/v/k5WdfpM-E8U">Full Screen</a> <a href="https://www.youtube.com/watch?v=k5WdfpM-E8U">Alternate</a>

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Alpha-amylase inhibitory activities of ascidians in the treatment of diabetes mellitus

Cited by 5 publications

References 3 publications

In Vitro Studies to Assess the Antidiabetic Potential of Schleichera Oleosa (Lour) Oken Leaves

In Vitro Studies to Assess the Antidiabetic Potential of Schleichera Oleosa (Lour) Oken Leaves

Antidiabetic Effects of the Ethanolic Root Extract ofUvaria chamaeP. Beauv (Annonaceae) in Alloxan-Induced Diabetic Rats: A Potential Alternative Treatment for Diabetes Mellitus

Synthesis, characterization and evaluation of antidiabetic activity of novel indoline derivatives

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