Alpha-glucosidase from the hepatopancreas of the shrimp,Penaus vannamei (Crustacea-Decapoda)

Chevalier, Patrick Le; Wormhoudt, Alain Van

doi:10.1002/(sici)1097-010x(19980415)280:6<384::aid-jez2>3.0.co;2-j

Cited by 25 publications

(15 citation statements)

References 39 publications

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“…The coding region of the shrimp alphaglucosidase cDNA reveals a protein of 919 amino acids (without the first methione) with a calculated molecular mass of 103,134 Da. This result confirms the molecular mass estimation by SDS PAGE with the purified protein [13]. A putative signal peptide corresponds to the first first 21 amino acids.…”

Section: Discussionsupporting

confidence: 86%

“…The first forward primer was designed from the conservative catalytic-site sequence WIDMNE (5%TGGMTNGAYATGAAYGAR3%) of alpha-glucosidase. The second oligonucleotide (reverse primer) was composed of 22 bases (5%GRTTYC-TNSWRAANGGRTARAA 3%) based on part of the amino acid sequence which we obtained previously (HFYPFSRNH) [13]. After 35 cycles of amplification, a strong DNA band of about 550 bp was specifically obtained for the shrimp digestive gland RNAs.…”

Section: Resultsmentioning

confidence: 99%

“…Two oligonucleotides were synthesized (Cybergene, France). The first was based on the partial amino acid sequence of the purified protein [13]. The second was designed as deduced from the amino acid sequence which is highly conserved in the catalytic site of other glucosidases.…”

Section: Methodsmentioning

confidence: 99%

“…Alpha-glucosidase has been purified to homogeneity from the digestive gland of Litopenaeus 6annamei [13]. In contrast to other known glucosidases, no alpha-1-6 glucose link hydrolysis activity has been observed in the shrimp purified enzyme [6,11].…”

mentioning

confidence: 99%

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Molecular cloning of a cDNA encoding alpha-glucosidase in the digestive gland of the shrimp, Litopenaeus vannamei

Chevalier¹,

Sellos

Wormhoudt

2000

CMLS, Cell. Mol. Life Sci.

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The complete sequence of the 3-kb cDNA and the 5' genomic structure are reported for the gene encoding the shrimp alpha-glucosidase. Alpha-glucosidase cDNA was isolated from a shrimp digestive gland cDNA library. The 2830-base pair cDNA contains an open reading frame that encodes 919 amino acids. The shrimp alpha-glucosidase cDNA shows a high level of identity with that of the human sucrase-isomaltase, human maltase-glucoamylase, and human acid lysosomal alpha-glucosidase, indicating that the protein shares the same structural domains. The similarities among these proteins are found as clusters and characterize the glycosyl hydrolase family 31. To our knowledge, this is the first report to describe a satellite sequence in the 5' genomic structure before the TATA box in an invertebrate sequence.

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Section: Discussionsupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular cloning of a cDNA encoding alpha-glucosidase in the digestive gland of the shrimp, Litopenaeus vannamei

Chevalier¹,

Sellos

Wormhoudt

2000

CMLS, Cell. Mol. Life Sci.

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“…In addition, soy protein concentrate (SPC) is difficult to produce and expensive for shrimp feed formulation. High levels of complex carbohydrates also represent an important obstacle to shrimp aquaculture, due to shrimp’s limited carbohydrates digestion capabilities [13–15]. …”

Section: Introductionmentioning

confidence: 99%

Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

et al. 2011

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Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids) and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs); Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM)—carbohydrates (CHO) basal feed. Treatment 2 (B + Bm); Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B); SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C); fishmeal commercial feed (FM) was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone) Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs); additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B) presented smaller growth and lower feed conversion ratio (FCR). Shrimps fed with the fishmeal commercial feed (C) presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

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Functional Feeds in Aquaculture

Soto

Paniagua‐Michel

López

et al. 2015

Hb25_Springer Handbook of Marine Biotechnology

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Alpha-glucosidase from the hepatopancreas of the shrimp,Penaus vannamei (Crustacea-Decapoda)

Cited by 25 publications

References 39 publications

Molecular cloning of a cDNA encoding alpha-glucosidase in the digestive gland of the shrimp, Litopenaeus vannamei

Molecular cloning of a cDNA encoding alpha-glucosidase in the digestive gland of the shrimp, Litopenaeus vannamei

Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

Functional Feeds in Aquaculture

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