Alpha irradiation of parts of single cells in tissue cultures

Munro, T.R.

doi:10.1016/0014-4827(58)90101-0

Cited by 17 publications

(2 citation statements)

References 20 publications

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“…Munro who innovatively delivered a particles to a selected part of cells using polonium deposited micro-needle [8]. It is gaining popularity for its small physical dimensions and applicability in general laboratories.…”

Section: Introductionmentioning

confidence: 99%

An integrated on-line irradiation and in situ live cell imaging system

Liang

Wang

et al. 2015

Nuclear Instruments and Methods in Physics Research Section B:

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Section: Introductionmentioning

confidence: 99%

An integrated on-line irradiation and in situ live cell imaging system

Liang

Wang

et al. 2015

Nuclear Instruments and Methods in Physics Research Section B:

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“…One of the first examples of this approach was the use of a polonium-coated tungsten microneedle to deliver α particles to living cells (4). Recently, Steeb et al (5) described an updated version of the microirradiator concept based on the deposition of isotopes by electroplating on a microelectrode enveloped by a glass capillary.…”

Section: Introductionmentioning

confidence: 99%

Use of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response

Zhi-min

Kuhne

Steeb

et al. 2010

Nucleic Acids Research

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Eukaryotic cells begin to assemble discrete, nucleoplasmic repair foci within seconds after the onset of exposure to ionizing radiation. Real-time imaging of this assembly has the potential to further our understanding of the effects of medical and environmental radiation exposure. Here, we describe a microirradiation system for targeted delivery of ionizing radiation to individual cells without the need for specialized facilities. The system consists of a 25-micron diameter electroplated Nickel-63 electrode, enveloped in a glass capillary and mounted in a micromanipulator. Because of the low energy of the beta radiation and the minute total amount of isotope present on the tip, the device can be safely handled with minimum precautions. We demonstrate the use of this system for tracking assembly of individual repair foci in real time in live U2OS human osteosarcoma cells. Results indicate that there is a subset of foci that appear and disappear rapidly, before a plateau level is reached ∼30 min post-exposure. This subset of foci would not have been evident without real-time observation. The development of a microirradiation system that is compatible with a standard biomedical laboratory expands the potential for real-time investigation of the biological effects of ionizing radiation.

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The muscular membrane and calcium activation of the contractile system of a lamellibranch smooth muscle (ABRM)

Baguet

Marchand-Dumont

1975

Pflugers Arch.

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1. Thin bundles of fresh ABRM treated by EDTA solution or Triton x-100 0.1% can be brought like glycerol-extracted fibres through contraction-relaxation cycles by changing the free Ca2+ level of the bathing medium for 10-6 M to 10-9 M. The kinetics of isometric force development and relaxation has been studied in the two conditions i.e ionic strength 0.06 pH 6.5 and ionic strength 0.28, pH 7.0 which are known to induce the catch-state in glycerol extracted fibres. When the low Ca-2+ solution (10-9M) is substituted for the high Ca-2+ solution (10-6M) immediately after the maximal force has been developed, relaxation occurs at a higher rate at ionic strength 0928 and pH 6.5. In this last condition, no tension redevelops after a quick release applied during the slow relaxation. 2. During the plateau, in presence of Ca-2+ 10-6 M. a quick release applied at any time is followed by a large redevelopment of tension at ionic strength 0.06 and pH 6.5. At ionic strength 0.28 and pH 7.0, the tension redevelops only during the decreased from 10-6 to 10-9 M without any change in the time course of the tension maintained. 3. These results suggest that in EDTA and Triton X-100 treated fibres of Abrm, the catch-state is spontaneously induced after the activation of the contractile mechanism under the same conditions of ionic strengh and pH as in glycerol extracted fibres. However, in the EDTA and Triton treated fibres, the presence of a high free Ca-2+ level is not necessary to maintain the tension in the catch-state induced at ionic strength 0.28 and pH 7.0.

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Alpha irradiation of parts of single cells in tissue cultures

Cited by 17 publications

References 20 publications

An integrated on-line irradiation and in situ live cell imaging system

An integrated on-line irradiation and in situ live cell imaging system

Use of a microscope stage-mounted Nickel-63 microirradiator for real-time observation of the DNA double-strand break response

The muscular membrane and calcium activation of the contractile system of a lamellibranch smooth muscle (ABRM)

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