Alpha-lipoic acid affects the oxidative stress in various brain structures in mice with methionine and choline deficiency

Abstract

Deficiency in methionine or choline can induce oxidative stress in various organs such as liver, kidney, heart, and brain. This study was to examine the effects of alpha-lipoic acid (LA) on oxidative stress induced by methionine and choline deficiency (MCD) in several brain structures. Male mice C57BL/6 (n ¼ 28) were divided into four groups: (1) control -continuously fed with standard chow; (2) LA -fed with standard chow and receiving LA; (3) MCD2 -fed with MCD diet for two weeks, and (4) MCD2þLA -fed with MCD diet for two weeks and receiving LA (100 mg/kg/day intraperitonealy [i.p.]). Brain tissue (cortex, hypothalamus, striatum and hippocampus) was taken for determination of oxidative stress parameters. MCD diet induced a significant increase in malondialdehyde and NOx concentration in all brain regions, while LA restored their content to normal values. Similar to this, in MCD2 group, activity of total SOD, MnSOD, and Cu/ZnSOD was reduced by MCD diet, while LA treatment improved their activities in all brain structures. Besides, in MCD2 group a decrease in catalase activity in cortex and GSH content in hypothalamus was evident, while LA treatment induced an increase in catalase activity in cortex and striatum and GSH content in hypothalamus. LA treatment can significantly reduce lipid peroxidation and nitrosative stress, caused by MCD diet, in all brain regions by restoring antioxidant enzymes activities, predominantly total SOD, MnSOD, and Cu/ZnSOD, and to a lesser extent by modulating catalase activity and GSH content. LA supplementation may be used in order to prevent brain oxidative injury induced by methionine and choline deficiency.

Protective effects of alpha lipoic acid on high glucose-induced neurotoxicity in PC12 cells

“…Studies performed using in vivo and in vitro showed that ALA can prevent stress oxidative by inducing of enzymatic antioxidant activity (SOD, catalase and GSH) as well as enhancing the capacity of free radical scavenging and by blocking lipid peroxidation (Abdou and Abdel-Daim 2014;Castro et al 2014;Gorąca et al 2013;Xia et al 2014). Because of its antioxidant activity, ALA acts as a neuroprotective agent in various neurodegenerative diseases related to stress oxidative stress (Boyaci et al 2014;Dalazen et al 2014;Demir et al 2014;Jalali-Nadoushan and Roghani 2013;Li et al 2013;Veskovic et al 2014).…”

Synergistic Effect of Quercetin andα-Lipoic Acid on Aluminium Chloride Induced Neurotoxicity in Rats

“…Though earlier studies [ 45 , 46 ] have shown AlCl 3 to inhibit catalase activity in rat brain, in the present study there was an increase in brain catalase activity in AlCl 3 treated rats. This variation may be explained by differential effects of oxidative stress on catalase activities in various regions of brain [ 47 ]. Studies of Singla and Dhawan 2014 [ 48 ] have shown that AlCl 3 causes an increase in catalase activity in rat brain.…”

Lipoic acid and pentoxifylline mitigate nandrolone decanoate-induced neurobehavioral perturbations in rats via re-balance of brain neurotransmitters, up-regulation of Nrf2/HO-1 pathway, and down-regulation of TNFR1 expression

“…Previous studies demonstrated that lipoic acid may cross the blood brain barrier, reduce MDA formation and enhance the activity of brain catalase and other antioxidant enzymes (Veskovic et al, 2015). In addition, lipoic acid is a thiol compound that possesses direct free radical scavenging activity (Shila et al, 2005).…”