Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen

Breininger, Elizabeth; Beorlegui, N.; O’Flaherty, Cristián; Beconi, M.T.

doi:10.1016/j.theriogenology.2004.08.016

Cited by 135 publications

(110 citation statements)

References 28 publications

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“…Moreover, reduction in amount of H 2 O 2 in this experiment in response to α-tocopherol may be due to potential of α-tocopherol to the phenoxyl radicals stabilization. Similar results have also been reported by Breininger et al (2005), who stated that α-tocopherol suppressed the ROS in boar spermatozoa after thawing.…”

Section: Discussionsupporting

confidence: 91%

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Motemani

Chamani

Sharafi

et al. 2017

Span. j. agric. res.

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This study was conducted to investigate the effects of different levels of α-tocopherol in cryopreservation media on the bull sperm quality after thawing. Semen samples were collected from six Holstein bulls using artificial vagina twice a week. Semen samples were pooled to eliminate individual differences and then divided into four equal parts for freezing with extenders containing different concentrations of α-tocopherol according to experimental groups as follows: 0 (control), 1.2 mM (E1), 2.4 mM (E2) and 4.8 mM (E4). Motion characteristics, viability, plasma membrane functionality, lipid peroxidation and H 2 O 2 status were determined after thawing. Results showed that malondialdehyde (MDA) concentration was significantly lower in E4 (6.1±0.6 nmol/mL) than E1 and E2 extenders (8.1±0.6 nmol/mL and 8±0.6 nmol/mL, respectively). Also, the lowest significant concentration of H 2 O 2 was observed in E4 (3.2±0.13 nmol/mL) compared to E1 (4 ±0.1 nmol/mL), E2 (5.3± 0.1 nmol/mL) and control (6.7±0.1 nmol/mL). Moreover, E2 and E4 produced the highest significant motility (74.2±1.6%, 75.9±1.6%), viability (78.2±1.8%, 76.1±1.8%) and membrane functionality (73±1.6%, 70.5±1.6%) compared to other groups. It can be concluded that α-tocopherol at the concentration of 4.8 mM can be an efficient antioxidant additive in Bioxcell extender for cryopreservation of bull semen.Additional key words: antioxidant; bioxcell; lipid peroxidation; sperm; H 2 O 2 .Correspondence should be addressed to Mohammad Chamani: m.chamani@srbiau.ac.ir Abbreviations used: LPO (lipid peroxidation); MDA (malondialdehyde); ROS (reactive oxygen species).Funding: The authors received no specific funding for this work.

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Section: Discussionsupporting

confidence: 91%

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Motemani

Chamani

Sharafi

et al. 2017

Span. j. agric. res.

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“…In boar, Breininger et al (2005) found that 200 mg/ml of vitamin E is the optimum concentration for motility and resistance to lipid peroxidation in samples of thawed semen. In the present work, both vitamin E (400 mg/ml) and IGF-I were effective in reducing the MDA production during 120 min of incubation time at 378C of boar semen after 72 h of storage at 158C.…”

Section: Discussionmentioning

confidence: 99%

“…During the storage of semen, in general way, antioxidants, such as vitamin E, are able to suppress lipid peroxidation by the capture of peroxyl radicals involved in the peroxidation chain (Wolf et al, 1998). In this way, the addition of vitamin E to diluents may increase sperm's resistance to lipid peroxidation in boars (Cerolini et al, 2000;Johannisson et al, 2003;Breininger et al, 2005). Therefore, this technique could be a method that increases the fertilising capacity of cooled semen used for artificial insemination.…”

Section: Introductionmentioning

confidence: 99%

Effect of the addition of IGF-I and vitamin E to stored boar semen

Mendez

Zangerônimo

Rocha

et al. 2013

Animal

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The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 158C, in conjunction with the addition of different amounts of vitamin E (a-tocopherol). Semen samples (n 5 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 mg/ml. Immediately after processing and after the doses had been stored at 158C for 24 or 72 h, samples were warmed at 378C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 mg/ml of vitamin E to diluted semen reduced ( P , 0.01) the malondialdehyde (MDA) production in boar semen stored at 158C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 378C. In these conditions, IGF-I also reduced ( P , 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced ( P 5 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 mg/ml of vitamin E reduces the MDA production in boar semen stored at 158C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 378C.

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“…vitamin E, glutathione, taurine) in the freezing extenders of frozen boar semen in an attempt to minimize the detrimental effect of ROS, which occurs during the freezing process [11][12][13][14]. Funahashi and Sano [15] demonstrated that a supplement of L-cysteine (5 mmol L −1 ) could improve the viability and progressive motility of fresh extended boar semen.…”

Section: Introductionmentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen

Cited by 135 publications

References 28 publications

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Effect of the addition of IGF-I and vitamin E to stored boar semen

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

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