Alpha-tocopheryl acetate is absorbed and hydrolyzed by Caco-2 cells

Brisson, Lydie; Castan, Stéphane; Fontbonne, Hervé; Nicoletti, Cendrine; Puigserver, Antoine; Ajandouz, El Hassan

doi:10.1016/j.chemphyslip.2008.04.002

Cited by 34 publications

(20 citation statements)

References 24 publications

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“…In addition, the ratio of hydrolysate amount to the total uptake amounts was approximately 5 . These results suggested that the cholesterol esterase activity for the hydrolysis of LevLF 4 and LF 5 was low in the Caco-2 cells used in the present study as previously reported 40 .…”

Section: Cellular Uptake Of Micellar Fucoxanthin Derivativessupporting

confidence: 74%

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Fucoxanthin Derivatives: Synthesis and their Chemical Properties

2015

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Section: Cellular Uptake Of Micellar Fucoxanthin Derivativessupporting

confidence: 74%

“…In addition, the yields were calculated from the area under the peak. The results are shown in 40,000 U/L penicillin, 40 mg/L streptomycin and 0.1 nM nonessential amino acids. Cells were kept at 37 in a humidified atmosphere of 95 air and 5 CO 2 .…”

Section: Enzymatic Hydrolysis Assaymentioning

confidence: 99%

Fucoxanthin Derivatives: Synthesis and their Chemical Properties

2015

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“…In vitro analysis of ␣-tocopherol cellular uptake by Caco-2 cells revealed that uptake was concentration-dependent (Reboul et al, 2006;Brisson et al, 2008;Narushima et al, 2008). Two carriers have been investigated for their possible role in ␣-tocopherol transport: NPC1L1 (Narushima et al, 2008) and scavenger receptor class B type 1 (SR-B1; Reboul et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Intestinal Absorption of γ-Tocotrienol Is Mediated by Niemann-Pick C1-Like 1: In Situ Rat Intestinal Perfusion Studies

Abuasal

Sylvester²,

Kaddoumi

2010

Drug Metab Dispos

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ABSTRACT:␥-Tocotrienol (␥-T3) is a member of the vitamin E family that displays potent anticancer activity and other therapeutic benefits. The objective of this study was to evaluate ␥-T3 intestinal uptake and metabolism using the in situ rat intestinal perfusion model. Isolated segments of rat jejunum and ileum were perfused with ␥-T3 solution, and measurements were made as a function of concentration (5-150 M). Intestinal permeability (P eff ) and metabolism were studied by measuring total compound disappearance and major metabolite, 2,7,8-trimethyl-2-(␤-carboxy-ethyl)-6-hydroxychroman, appearance in the intestinal lumen. ␥-T3 and metabolite levels were also determined in mesenteric blood. The P eff of ␥-T3 was similar in both intestinal segments and significantly decreased at concentrations >25 M in jejunum and ileum (p < 0.05), whereas metabolite formation was minimal and mesenteric blood concentrations of ␥-T3 and metabolite remained very low. These results indicate that ␥-T3 intestinal uptake is a saturable carrier-mediated process and metabolism is minimal. Results from subsequent in situ inhibition studies with ezetimibe, a potent and selective inhibitor of Niemann-Pick C1-like 1 (NPC1L1) transporter, suggested ␥-T3 intestinal uptake is mediated by NPC1L1. Comparable findings were obtained when Madin-Darby canine kidney II cells that express endogenous NPC1L1 were incubated with increasing concentrations of ␥-T3 or ␥-T3 with increasing concentrations of ezetimibe. The present data show for the first time that ␥-T3 intestinal absorption is partly mediated by NPC1L1.

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“…Nonetheless, other luminal candidate enzymes exist, e.g. phospholipase B, and the participation of brush border enzyme has been suggested as well (32,(35)(36)(37)(38). Actually, Nagy et al have recently shown that α-tocopherol and α-tocopheryl acetate exhibited the same bioavailability in the absence of digestive enzymes and bile salts in healthy subjects, even at supplemental doses (38).…”

Section: Vitamin E Fate In the Lumen Of The Upper Gastrointestinal Trmentioning

confidence: 99%