Alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. II. Identification using a sequence-specific monoclonal antibody.

Kobrin, Michael S.; Samsoondar, James; Kudlow, Jeffrey E.

doi:10.1016/s0021-9258(18)66885-1

Cited by 78 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TGFalpha which apparently shares its receptor with EGF, is also a mitogen for cells of ectodermal and mesodermal origin (Schreiber et al ., 1986) . The TGFalpha mRNA is expressed in several areas of the brain (Kobrin et al ., 1986;Wilcox and Derynck, 1988), and TGFalpha induces neurite outgrowth in PC12 cells (Zhang, 1990) . Finally, activin, which was first isolated as a peptide hormone that stimulates the release of FSH from pituitary cells (Mason et al ., 1985 ;Vale et al ., 1986), causes the survival of neurogenic P19 teratoma cells and some types of nerve cells (Schubert et al ., 1990) .…”

Section: Survival In Serumfree Mediummentioning

confidence: 99%

Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells.

Kimura

Schubert

1992

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Schwannoma-derived growth factor (SDGF) was initially isolated from Schwannoma cells as a mitogen for glial cells and fibroblasts . The present data show that SDGF causes the morphological and molecular differentiation of rat PC12 cells in a manner similar to, but distinguishable from nerve growth factor (NGF) . It also promotes PC12 survival in serum-free conditions . SDGF induced changes include neurite outgrowth and the induction of the mRNAs for GAP-43 and transin, proteins which are highly expressed in

show abstract

Section: Survival In Serumfree Mediummentioning

confidence: 99%

Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells.

Kimura

Schubert

1992

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…TGF-a binds to EGF-R with an affinity comparable to that of EGF and activates EGF-R PTK activity (8,9). TGF-oe was originally detected in culture supernatants of transformed rodent fibroblasts (10)(11)(12), and is also produced by a variety of normal cell types (13)(14)(15)(16). TGF-oe is mitogenic for cultured fibroblasts (10), endothelial cells (17), and epidermal keratinocytes (18).…”

mentioning

confidence: 99%

Regulation of cytokine production in the human thymus: epidermal growth factor and transforming growth factor alpha regulate mRNA levels of interleukin 1 alpha (IL-1 alpha), IL-1 beta, and IL-6 in human thymic epithelial cells at a post-transcriptional level.

Lazorick

Whichard

et al. 1991

The Journal of Experimental Medicine

View full text Add to dashboard Cite

SllnunaryHuman thymic epithelial (TE) cells produce interleukin loe (Ibloe), I1:1~, and 11:6, cytokines that are important for thymocyte proliferation. The mRNAs for these cytokines are short-lived and are inducible by multiple stimuli. Thus, the steady-state levels for II.-1 and I1:6 mRNAs are critical in establishing the final cytokine protein levels. In this study we have evaluated the effect of epidermal growth factor (EGF), a growth factor for TE cells, and its homologue transforming growth factor oe (TGF-a), on primary cultures of normal human TE cells for the levels of I1:1oe, I1:1~, 1I.-6, and TGF-oe mRNA. We showed that TE cells expressed EGF receptors (EGF-R) in vitro and in vivo, and that treatment of TE cells with EGF or TGF-oe increased II.,1 and II.-6 biological activity and mRNA levels for I1:1oe, IL-lfl, and II-.6. Neither EGF nor TGF-c~ increased transcription rates of I1:1oe, II--18, and I1-.6 genes, but rather both EGF and TGF-ee increased cytokine mRNA stability. By indirect immunofluorescence assay, TGF-oe was localized in medullary TE cells and thymic Hassall's bodies while EGF-R was localized to TE cells throughout the thymus. Thus, TGF-oe and EGF are critical regulatory molecules for production of TE cell-derived cytokines within the thymus and may function as key modulators of human T cell development in vivo.

show abstract

“…More recently, TGF-a has been demonstrated in certain normal tissues and cell types as well, including normal human skin 1 Abbreviations used in this paper: DAPI, 4',6-diamidino-2-phenylindole; EGF, epidermal growth factor; h, human; mRNA, messenger RNA . keratinocytes (4,5), bovine anterior pituitary cells (6), rat material decidua (7), mouse blastocysts (8), and activated macrophages (9,10) . These findings suggest that TGF-a is not only involved in neoplasms but may also contribute to interactions among normal cells, possibly through autocrine and/or paracrine mechanisms (11).…”

mentioning

confidence: 99%

Human eosinophils express transforming growth factor alpha.

Wong

Weller

Galli

et al. 1990

The Journal of Experimental Medicine

213

View full text Add to dashboard Cite

SummaryTransforming growth factor a (TGF-a) is a pleuripotential cytokine with diverse biological effects, including the ability to influence the proliferation of normal cells or neoplastic epithelial cells. Eosinophils are a subset of granulocytes that normally enter the peripheral tissues, particularly those beneath gastrointestinal, respiratory, and urogenital epithelium, where they reside in close proximity to the epithelial elements . In this study, we demonstrate that the great majority of eosinophils infiltrating the interstitial tissues adjacent to two colonic adenocarcinomas and two oral squamous cell carcinomas labeled specifically by in situ hybridization with a 35S-riboprobe for human TGF-a (hTGF-a) . No other identifiable leukocytes in these lesions contained detectable hTGF-a mRNA. We also examined leukocytes purified from a patient with the idiopathic hypereosinophilic syndrome. 80% of these eosinophils, but none of the patient's neutrophils or mononuclear cells, were positive for hTGF-a mRNA by in situ hybridization, and 55% of these eosinophils were positive by immunohistochemistry with a monoclonal antibody directed against the COOH terminus of the mature hTGF-a peptide. Finally, the identification of the purified eosinophil-associated transcript as hTGF-ca was confirmed by polymerase chain reaction product restriction enzyme analysis followed by Southern blot hybridization . In contrast to eosinophils from the patient with hypereosinophilic syndrome, the peripheral blood eosinophils from only two of seven normal donors had detectable TGF-a mRNA and none ofthese eosinophils contained immunohistochemically detectable TGF-a product. Taken together, these findings establish that human eosinophils can express TGF-a, but suggest that the expression of TGF-a by eosinophils may be under microenvironmental regulation. Demonstration of TGF-a production by tissueinfiltrating eosinophils and the eosinophils in the hypereosinophilic syndrome identifies a novel mechanism by which eosinophils might contribute to physiological, immunological, and pathological responses .

show abstract

Alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. II. Identification using a sequence-specific monoclonal antibody.

Cited by 78 publications

References 27 publications

Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells.

Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells.

Regulation of cytokine production in the human thymus: epidermal growth factor and transforming growth factor alpha regulate mRNA levels of interleukin 1 alpha (IL-1 alpha), IL-1 beta, and IL-6 in human thymic epithelial cells at a post-transcriptional level.

Human eosinophils express transforming growth factor alpha.

Contact Info

Product

Resources

About