Alphafetoprotein and neural tube defects

Brock, D. J. H.

doi:10.1136/jcp.s3-10.1.157

Cited by 6 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Historically, prenatal diagnostic testing for fetal aneuploidy was offered for advanced maternal age because of the well‐recognized incidence of aneuploidy in that population . However, the use of maternal age as a primary screening variable changed with the discovery of maternal biochemical markers and sonographic markers associated with aneuploidy . The now well‐recognized nuchal translucency (NT) assessment in the first trimester and association with aneuploidy was described in the early 1990s in Europe and became readily available in the United States in the early 2000s .…”

Section: Introductionmentioning

confidence: 99%

Trends in invasive prenatal diagnostic testing at a single institution

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Trends in invasive prenatal diagnostic testing at a single institution

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The members of albumin superfamily have been found to act as markers in various disease states in humans. AFP in maternal serum is an indicative of Down's syndrome and neural tube defects in the fetus [ 24 , 25 ]. AFP levels are elevated in patients with high risk for hepatocellular carcinoma.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Synonymous Codon Usage Bias Pattern in Human Albumin Superfamily

Mirsafian

Ripen

Singh

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

Synonymous codon usage bias is an inevitable phenomenon in organismic taxa across the three domains of life. Though the frequency of codon usage is not equal across species and within genome in the same species, the phenomenon is non random and is tissue-specific. Several factors such as GC content, nucleotide distribution, protein hydropathy, protein secondary structure, and translational selection are reported to contribute to codon usage preference. The synonymous codon usage patterns can be helpful in revealing the expression pattern of genes as well as the evolutionary relationship between the sequences. In this study, synonymous codon usage bias patterns were determined for the evolutionarily close proteins of albumin superfamily, namely, albumin, α-fetoprotein, afamin, and vitamin D-binding protein. Our study demonstrated that the genes of the four albumin superfamily members have low GC content and high values of effective number of codons (ENC) suggesting high expressivity of these genes and less bias in codon usage preferences. This study also provided evidence that the albumin superfamily members are not subjected to mutational selection pressure.

show abstract

“…Alpha-fetoprotein (AFP) is a plasma protein mainly found in human fetuses. During pregnancy, elevated AFP concentrations in maternal serum may indicate spina bifida and anencephaly, 2 whereas decreased AFP levels in the second trimester of pregnancy are evaluated within risk assessment for trisomy 21 (Down syndrome) in combination with human chorionic gonadotropin beta (hCG + β) among other parameters, such as gestational age and maternal weight. 3 In addition, AFP is an important diagnostic tumor-specific biomarker for different types of cancers.…”

Section: ■ Introductionmentioning

confidence: 99%

Metal Nanoparticles/MoS₂ Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection

Sánchez‐Iglesias

Silvestri

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The detection of cancer biomarkers at an early stage of tumor development is vital for effective diagnosis and treatment of cancer. Current diagnostic tools can often detect cancer only when the biomarker levels are already too high, so that the tumors have spread and treatments are less effective. It is urgent therefore to develop highly sensitive assays for the detection of such biomarkers at the lowest possible concentration. In this context, we developed a sandwich immunoassay based on surface-enhanced Raman scattering (SERS) for the ultrasensitive detection of α-fetoprotein (AFP), which is typically present in human serum as a biomarker indicative of early stages of hepatocellular carcinoma. In the immunoassay design, molybdenum disulfide (MoS2) modified with a monoclonal antibody was used as a capture probe for AFP. A secondary antibody linked to an SERS-encoded nanoparticle was employed as the Raman signal reporter, that is, the transducer for AFP detection. The sandwich immunocomplex “capture probe/target/SERS tag” was deposited on a silicon wafer and decorated with silver-coated gold nanocubes to increase the density of “hot spots” on the surface of the immunosensor. The developed SERS immunosensor exhibits a wide linear detection range (1 pg mL–1 to 10 ng mL–1) with a limit of detection as low as 0.03 pg mL–1 toward AFP with good reproducibility (RSD < 6%) and stability. These parameters demonstrate that the proposed immunosensor has the potential to be used as an analytical platform for the detection of early-stage cancer biomarkers in clinical applications.

show abstract

Alphafetoprotein and neural tube defects

Cited by 6 publications

References 44 publications

Trends in invasive prenatal diagnostic testing at a single institution

Trends in invasive prenatal diagnostic testing at a single institution

A Comparative Analysis of Synonymous Codon Usage Bias Pattern in Human Albumin Superfamily

Metal Nanoparticles/MoS₂ Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection

Contact Info

Product

Resources

About

Alphafetoprotein and neural tube defects

Cited by 6 publications

References 44 publications

Trends in invasive prenatal diagnostic testing at a single institution

Trends in invasive prenatal diagnostic testing at a single institution

A Comparative Analysis of Synonymous Codon Usage Bias Pattern in Human Albumin Superfamily

Metal Nanoparticles/MoS2 Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection

Contact Info

Product

Resources

About

Metal Nanoparticles/MoS₂ Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection