Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture

Pedersen, Charlotte; Hansen, Conny Wang; Brandt, Kirsten; Kristiansen, Kell

doi:10.1016/s0304-4238(96)00917-x

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…The second, next higher axillary bud at the vertical shoot (2 nd axbud) usually remains dormant because of strong apical dominance but occasionally grows into a lateral rhizome (Van Schaik 1998). In spite of a low multiplication rate (1.2 -1.8 per 4 weeks), forced outgrowth of the 2 nd axbud is the preferred method for micropropagation of Alstroemeria (Pedersen et al 1996, Buitendijk et al 1992.…”

Section: Introductionmentioning

confidence: 99%

Hormonal control of the outgrowth of axillary buds in Alstroemeria cultured in vitro

Pumisutapon

Visser²,

Klerk³

2011

Biologia plant.

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We study apical dominance in Alstroemeria, a plant with an architecture very different from the model species used in research on apical dominance. The standard explant was a rhizome with a tip and two vertically growing shoots from which the larger part had been excised leaving ca. 1 cm stem. The axillary buds that resumed growth were located at this 1-cm stem just above the rhizome. They were released by removal of the rhizome tip and the shoot tips. Replacement of excised tips by lanolin with indole-3-butyric acid (IBA) restored apical dominance. The auxin transport inhibitors 2,3,5-triiodobenzoic acid (TIBA) and N-1-napthylphthalamic acid (NPA) reduced apical dominance. 6-Benzylaminopurine (BAP) enhanced axillary bud outgrowth but the highest concentrations (> 9 µM) caused fasciation. Thidiazuron (TDZ) did not show improvement relative to BAP. Even though the architecture of Alstroemeria and the model species are very different, their hormonal mechanisms in apical dominance are for the greater part very similar.

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Section: Introductionmentioning

confidence: 99%

Hormonal control of the outgrowth of axillary buds in Alstroemeria cultured in vitro

Pumisutapon

Visser²,

Klerk³

2011

Biologia plant.

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“…It is used for cut flowers or as potted or garden plants (Bridgen, 1997). Its hybrids can be propagated by seeds, but its progeny is highly heterogeneous (Pedersen et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

Pedraza‐Santos

López-Peralta

Hernández

et al. 2005

Plant Cell Tiss Organ Cult

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The common techniques for the in vitro production of Alstroemeria plants are based on rhizomes as explants, which have low multiplication rates and a high risk of carrying viral diseases. To overcome these problems, we developed a protocol for the in vitro regeneration of Alstroemeria cv.'Yellow King', by testing for shoot induction several explant sources (leaf, stem apices, rhizomes and immature inflorescence apices), temperature and light/dark regimes, hormone and salt concentrations. For shoot multiplication and rooting, several hormone concentrations were tested. We found that only the young floral apices produced adventitious shoots by direct organogenesis. The highest shoot induction rate (10.4 shoots per explant) was obtained by incubation in the dark for 15 days at 8°C followed by 15 days at 25°C and a 16-h/8-h light/ dark regime, on a Murashige and Skoog (1962) liquid medium at 50% of the salt concentration, supplemented with 2.5 mg l )1 KIN, 1.5 mg l )1 BA and 1.0 mg l )1 NAA, using a piece filter paper to support the explant. The highest shoot multiplication rate (9 shoots per explant) was obtained on a liquid MS medium at full strength supplemented only with BA at 1.0 mg l )1 . In vitro rooting of shoots was induced also on a liquid MS medium, either with or without plant hormones.

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“…In vitro methods have been successful for Alstroemeriaceae species [19][20][21], including micropropagation [22,23]. To achieve successful propagation using in vitro methods for any plant species, the growth media, pH, incubation temperature, light conditions, and type and concentration of hormones must be optimized [24,25].…”

Section: Introductionmentioning

confidence: 99%

Sexual and asexual reproductive aspects of Leontochir ovallei, a rare and endangered geophyte of the Atacama Desert

Vargas

Jofré

Navarrete

et al. 2018

Rev. Chil. de Hist. Nat.

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Background: "Garra de Leon" (Leontochir ovallei) is an ephemeral endangered Alstroemeriaceae species endemic to Chile. Despite many efforts to improve the conservation of this species, the stimulation of dormant seeds and the production of rhizomes under controlled conditions remain unexplored. The aims of this study were to examine the germination responses of L. ovallei seeds under different in vitro conditions and to evaluate the formation of viable rhizomes after transplantation from in vitro to ex vitro conditions. Methods: We evaluated five in vitro seed germination treatments: (1) acid scarification, (2) acid scarification followed by imbibition of seeds in aerated water, (3) imbibition of seeds in gibberellic acid, (4) clipping of seeds with a scalpel, and (5) seeds without any treatment (control). Seedlings obtained under in vitro conditions were transplanted to ex vitro conditions following a gradual acclimation process. After eight months, the number of rhizomes per plant was counted. To test asexual multiplication, each rhizome with its respective storage organ was divided using a scalpel and then left to rest for two years before subsequent evaluation of viability. After that period, the rhizomes were re-hydrated, and the emergence of plants after three months was evaluated.

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Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture

Cited by 13 publications

References 11 publications

Hormonal control of the outgrowth of axillary buds in Alstroemeria cultured in vitro

Hormonal control of the outgrowth of axillary buds in Alstroemeria cultured in vitro

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

Sexual and asexual reproductive aspects of Leontochir ovallei, a rare and endangered geophyte of the Atacama Desert

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